N-acetylation of α-synuclein enhances synaptic vesicle clustering mediated by α-synuclein and lysophosphatidylcholine DOI Open Access
Chuchu Wang, Chunyu Zhao, Xiao Hu

и другие.

Опубликована: Сен. 13, 2024

Post-translational modifications (PTMs) of α-synuclein (α-syn) such as acetylation and phosphorylation play important yet distinct roles in regulating α-syn conformation, membrane binding, amyloid aggregation. However, how PTMs regulate function presynaptic terminals remains unclear. Previously, we reported that clusters synaptic vesicles (SV) 1 , neutral phospholipid lysophosphatidylcholine (LPC) can mediate this clustering 2 . Here, based on our previous findings, further demonstrate N-terminal acetylation, which occurs under physiological conditions is irreversible mammalian cells, significantly enhances the functional activity SVs. Mechanistic studies reveal enhancement caused by N-acetylation-promoted insertion α-syn’s N-terminus increased intermolecular interactions LPC-containing membrane. Our work demonstrates N-acetylation fine-tunes α-syn–LPC interaction for mediating SV clustering.

Язык: Английский

Clinical glycoproteomics: methods and diseases DOI Creative Commons
Yujia Wang,

Kaixin Lei,

Lijun Zhao

и другие.

MedComm, Год журнала: 2024, Номер 5(10)

Опубликована: Окт. 1, 2024

Abstract Glycoproteins, representing a significant proportion of posttranslational products, play pivotal roles in various biological processes, such as signal transduction and immune response. Abnormal glycosylation may lead to structural functional changes glycoprotein, which is closely related the occurrence development diseases. Consequently, exploring protein can shed light on mechanisms behind disease manifestation pave way for innovative diagnostic therapeutic strategies. Nonetheless, study clinical glycoproteomics fraught with challenges due low abundance intricate structures glycosylation. Recent advancements mass spectrometry‐based have improved our ability identify abnormal glycoproteins samples. In this review, we aim provide comprehensive overview foundational principles recent glycoproteomic methodologies applications. Furthermore, discussed typical characteristics, underlying functions, diseases, brain cardiovascular cancers, kidney metabolic Additionally, highlighted potential avenues future glycoproteomics. These insights provided review will enhance comprehension methods diseases promote elucidation pathogenesis discovery novel biomarkers targets.

Язык: Английский

Процитировано

3
A tale of two sugars: O-GlcNAc and O-fucose orchestrate growth, development, and acclimation in plants DOI
Yalikunjiang Aizezi, Yizhong Yuan, Shou‐Ling Xu

и другие.

Trends in Biochemical Sciences, Год журнала: 2025, Номер unknown

Опубликована: Фев. 1, 2025

Язык: Английский

Процитировано

0
Glycosylation of serine/threonine-rich intrinsically disordered regions of membrane-associated proteins in streptococci DOI Creative Commons
Mohammad M. Rahman, Svetlana Zamakhaeva, Jeffrey S. Rush

и другие.

Nature Communications, Год журнала: 2025, Номер 16(1)

Опубликована: Апрель 29, 2025

Язык: Английский

Процитировано

0
N-acetylation of α-synuclein enhances synaptic vesicle clustering mediated by α-synuclein and lysophosphatidylcholine DOI Creative Commons
Chuchu Wang, Chunyu Zhao,

Xiao Hu

и другие.

eLife, Год журнала: 2024, Номер 13

Опубликована: Апрель 19, 2024

Previously, we reported that α-synuclein (α-syn) clusters synaptic vesicles (SV) Diao et al., 2013, and neutral phospholipid lysophosphatidylcholine (LPC) can mediate this clustering Lai 2023. Meanwhile, post-translational modifications (PTMs) of α-syn such as acetylation phosphorylation play important yet distinct roles in regulating conformation, membrane binding, amyloid aggregation. However, how PTMs regulate function presynaptic terminals remains unclear. Here, based on our previous findings, further demonstrate N-terminal acetylation, which occurs under physiological conditions is irreversible mammalian cells, significantly enhances the functional activity SVs. Mechanistic studies reveal enhancement caused by N-acetylation-promoted insertion α-syn’s N-terminus increased intermolecular interactions LPC-containing membrane. N-acetylation work shown to fine-tune interaction between LPC, mediating role vesicle clustering.

Язык: Английский

Процитировано

2
O-GlcNAcylation of YTHDF2 antagonizes ERK-dependent phosphorylation and inhibits lung carcinoma DOI Creative Commons
Jie Li, Wen Zhou, Jianzhi Zhang

и другие.

Fundamental Research, Год журнала: 2024, Номер unknown

Опубликована: Июль 1, 2024

The intracellular O-linked N-acetylglucosamine (O-GlcNAc) glycosylation mediates many signal transduction events and regulates tumorigenesis. Previously the RNA N6-methyladenosine (m6A) reader, YTH (YT521-B homology) domain 2 (YTHDF2), has been shown to be O-GlcNAcylated on Ser-263 during Hepatitis B virus (HBV) infection promote HBV-related hepatocellular carcinoma. Herein we mapped YTHDF2 O-GlcNAcylation at Thr-49 via electron-transfer dissociation mass spectrometry under unperturbed conditions. We show that antagonizes Extracellular-signal regulated kinase (ERK)-dependent phosphorylation Ser-39 promotes degradation. downstream signaling pathway of in lung carcinoma is thus upregulated, which leads downregulation c-Myc. further used mouse xenograft models YTHDF2-T49A mutants increased cancer size. Our work reveals a key role tumorigenesis suggests exerts distinct functions different biological stress.

Язык: Английский

Процитировано

2
Synthesis of Bodipy-Tagged Galactoconjugates and Evaluation of Their Antibacterial Properties DOI Creative Commons
Chiara M. A. Gangemi,

Maura Monforte,

Antonino Arrigo

и другие.

Molecules, Год журнала: 2024, Номер 29(10), С. 2299 - 2299

Опубликована: Май 14, 2024

As a development of our research on biocompatible glycoconjugate probes and specifically multi-chromophoric systems, herein, we report the synthesis early bactericidal tests two luminescent glycoconjugates whose basic structure is characterized by boron dipyrromethene difluoride (BODIPY) moieties three galactoside rings mounted an oligophenylene ethynylene (OPE) skeleton. BODIPY fluorophores have found widespread application in many branches biology last few decades. In particular, molecular platforms showing different groups unique photophysical behavior useful fluorescence imaging. Construction complex architecture new accomplished through convergent route that exploits series copper-free Heck-Cassar-Sonogashira cross-couplings. The great emergency due to proliferation bacterial infections, conjunction with growing antibiotic resistance, requires production multifunctional drugs efficient methods for their targeted delivery control bacteria-associated diseases. Preliminary studies properties as antibacterial agents against representatives Gram-negative (

Язык: Английский

Процитировано

1
N-acetylation of α-synuclein enhances synaptic vesicle clustering mediated by α-synuclein and lysophosphatidylcholine DOI Creative Commons
Chuchu Wang, Chunyu Zhao,

Xiao Hu

и другие.

eLife, Год журнала: 2024, Номер 13

Опубликована: Дек. 27, 2024

Previously, we reported that α-synuclein (α-syn) clusters synaptic vesicles (SV) Diao et al., 2013, and neutral phospholipid lysophosphatidylcholine (LPC) can mediate this clustering Lai 2023. Meanwhile, post-translational modifications (PTMs) of α-syn such as acetylation phosphorylation play important yet distinct roles in regulating conformation, membrane binding, amyloid aggregation. However, how PTMs regulate function presynaptic terminals remains unclear. Here, based on our previous findings, further demonstrate N-terminal acetylation, which occurs under physiological conditions is irreversible mammalian cells, significantly enhances the functional activity SVs. Mechanistic studies reveal enhancement caused by N-acetylation-promoted insertion α-syn’s N-terminus increased intermolecular interactions LPC-containing membrane. N-acetylation work shown to fine-tune interaction between LPC, mediating role vesicle clustering.

Язык: Английский

Процитировано

1
O-GlcNAcylation of YTHDF2 antagonizes ERK-dependent phosphorylation and inhibits lung carcinoma DOI Open Access
Jie Li, Wen Zhou, Jianzhi Zhang

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Сен. 10, 2023

SUMMARY The intracellular O-linked N-acetylglucosamine (O-GlcNAc) glycosylation mediates many signal transduction events and regulates tumorigenesis. Previously the RNA N6-methyladenosine (m 6 A) reader, YTH (YT521-B homology) domain 2 (YTHDF2), has been shown to be O-GlcNAcylated on Ser-263 during Hepatitis B virus (HBV) infection promote HBV-related hepatocellular carcinoma. Herein we mapped YTHDF2 O-GlcNAcylation at Thr-49 via electron-transfer dissociation mass spectrometry under unperturbed conditions. We show that antagonizes Extracellular-signal regulated kinase (ERK)-dependent phosphorylation Ser-39 promotes degradation. downstream signaling pathway of in lung carcinoma are thus upregulated, which leads downregulation c-Myc. further used mouse xenograft models YTHDF2-T49A mutants increased cancer size. Our work reveals a key role tumorigenesis suggests exerts distinct functions different biological stress.

Язык: Английский

Процитировано

2
O-GlcNAc regulates YTHDF1 and YTHDF3 activity DOI
Mary W. N. Burns, Jennifer J. Kohler

Nature Cell Biology, Год журнала: 2023, Номер 25(11), С. 1570 - 1572

Опубликована: Ноя. 1, 2023

Язык: Английский

Процитировано

2
A Patching and Coding Lipid Raft-Localized Universal Imaging Platform DOI Creative Commons

Tong Zhong,

Younan Chen,

Xiaomin Yan

и другие.

Chemical & Biomedical Imaging, Год журнала: 2023, Номер 2(2), С. 135 - 146

Опубликована: Ноя. 29, 2023

Lipid rafts (LRs) are relatively well-ordered functional microdomains in cell membranes and play an irreplaceable role physiological processes as a transduction platform for multiple signaling pathways. Due to their small size high spatiotemporal dynamics, it is difficult perform lipid raft-localized biomolecule imaging on the surface of living cells. Here, we report DNA nanotechnology-based reversible manipulation localized analysis rafts, which consists two modules: “patching coding probe pair” “fishing probe”. The pair generated by modifying different sets connectable structures raft-specific protein. After recognizing probes close proximity linked ligase reaction form raft identity (LR-ID) code. LR-ID strand patches stabilizes structure. Interestingly, formed can be depatched restriction endonucleases, providing first structure We also designed probe” with hairpin using aptamer that specifically bind target. cascade input signals “LR-ID” “target protein” generate “off–on” fluorescence switch, allowing dynamic monitoring target proteins rafts. By encoding arbitrary targets (in case glycans) have created universal platform. This work provides integrated analytical manipulative reveal associated pathways at molecular level.

Язык: Английский

Процитировано

2