Analytical Chemistry, Год журнала: 2024, Номер unknown

Опубликована: Дек. 2, 2024

Cell-cell interactions determine the activation state and function of cells. When host cells are exposed to stressors such as microorganisms, immune defense machinery is activated release H2O2, providing direct evidence relevant cellular physiological processes. Inspired by fact that peroxidase can catalyze proximity labeling in presence exogenous a stressor-actuated (SAPL) strategy developed report process information on cell–cell recording stress levels. The covalently modified with horseradish (HRP) H2O2 released response triggers HRP-based labeling. Using fungal mimic or live fungi stressors, levels different compared situ imaging signals. ability accumulate signals allows SAPL more sensitively differentiate between involving macrophage phenotypes. also powerful tool for real-time, monitoring effects surface modifications interactions. Thus, represents new perspective using endogenous effector molecules.

Язык: Английский