Integrated Metabolomics and Transcriptomic Analysis of Cellular Stress Responses: Elucidating Metabolic Pathways and Regulatory Networks DOI

Gul Rukh Askary,

Zafar U. Khan,

Syeda Samira Azim

и другие.

Indus journal of bioscience research., Год журнала: 2024, Номер 2(2), С. 1191 - 1197

Опубликована: Дек. 22, 2024

Cellular stress responses are vital for maintaining homeostasis and enabling adaptation to environmental physiological challenges. This study employed an integrated metabolomic transcriptomic approach investigate the regulatory networks metabolic pathways underlying these responses. Conducted between July 2023 December 2024 in Karachi, Pakistan, research utilized cutting-edge technologies, including real-time PCR, high-performance liquid chromatography (HPLC), gas chromatography-mass spectrometry (GC-MS), next-generation sequencing (NGS). Biological samples representing various conditions, such as oxidative stress, nutrient deprivation, stressors, were collected analyzed under strict ethical protocols. RNA (RNA-Seq) revealed 1,250 differentially expressed genes (DEGs), with notable upregulation of GPX1 SOD2, indicating enhanced detoxification stress. Concurrently, profiling identified significant alterations metabolites, elevated glutathione, lactate, proline, highlighting adaptive shifts glycolysis, reactive oxygen species (ROS) detoxification, osmoprotection. Integrative analysis using Weighted Gene Co-expression Network Analysis (WGCNA) pinpointed key hubs, HIF1A succinate, central nodes stress-specific networks. comprehensive multi-omics provided actionable insights into molecular mechanisms cellular The findings hold translational potential agriculture medicine, particularly enhancing crop resilience developing targeted therapies stress-related disorders. Despite limitations vitro models, this underscores value multi-layered omics analyses elucidating lays groundwork future integrative

Язык: Английский

Omics approaches in understanding the benefits of plant-microbe interactions DOI Creative Commons
Archana Jain, Surendra Sarsaiya,

Ranjan Singh

и другие.

Frontiers in Microbiology, Год журнала: 2024, Номер 15

Опубликована: Май 27, 2024

Plant-microbe interactions are pivotal for ecosystem dynamics and sustainable agriculture, influenced by various factors, such as host characteristics, environmental conditions, human activities. Omics technologies, including genomics, transcriptomics, proteomics, metabolomics, have revolutionized our understanding of these interactions. Genomics elucidates key genes, transcriptomics reveals gene expression dynamics, proteomics identifies essential proteins, metabolomics profiles small molecules, thereby offering a holistic perspective. This review synthesizes diverse microbial-plant interactions, showcasing the application omics in mechanisms, nitrogen fixation, systemic resistance induction, mycorrhizal association, pathogen-host Despite challenges data integration ethical considerations, approaches promise advancements precision intervention resilient agricultural practices. Future research should address challenges, enhance technology resolution, explore epigenomics, understand plant-microbe under conditions. In conclusion, technologies hold immense optimizing strategies fortifying alliances, paving way agriculture stewardship.

Язык: Английский

Процитировано

15

Rhizosphere Metabolite Dynamics in Continuous Cropping of Vineyards: Impact on Microflora Diversity and Co-occurrence Networks DOI
Rui Song,

Bihan Lv,

Zhouyang He

и другие.

Microbiological Research, Год журнала: 2025, Номер 296, С. 128134 - 128134

Опубликована: Март 7, 2025

Язык: Английский

Процитировано

0

Prioritizing Citrullus colocynthis accessions and fruit parts with promising antibacterial activity and adaption to the hot climate of the UAE for the future pharmaceutical industry DOI Creative Commons

Shaimaa Al Nablsi,

Ali El‐Keblawy, Rania Hamdy

и другие.

F1000Research, Год журнала: 2025, Номер 14, С. 277 - 277

Опубликована: Март 10, 2025

Background The rise of multidrug-resistant pathogens and emerging new microbes due to climate change highlight the urgent need for alternative antimicrobials. Plants, as they adapt environmental shifts, produce diverse metabolites with potent antibacterial properties, offering a sustainable source combat antibiotic resistance microbial threats. In this context, Citrullus colocynthis fruits, known their antimicrobial activity adaptation hot Gulf region, were screened identify accessions strong distinct metabolic profiles. Methods rinds pulps three C. effectiveness against Staphylococcus aureus Escherichia coli. Minimum inhibitory concentration (MIC50) tests determined levels, GC-MS/MS profiling analyzed chemical composition rind pulp extracts. Enrichment network analyses performed pathways potential bacterial targets. Results Rind extracts demonstrated stronger than pulp, accession S2 showing highest S. (MIC50 = 15.74 μg/ml), outperforming other accessions. Metabolic revealed metabolite clusters between containing unique compounds like butyric acid, α-linolenic β-sitosterol. analysis indicated that unsaturated fatty acid biosynthesis metabolism enriched in rind, supporting its potency. Network pinpointed synthase enzymes (FabZ, FabI, FabH) targets acids. Conclusions profiles especially S2, underscore plant-based nutraceuticals. Its adaptability properties present promising strategy combating pathogens.

Язык: Английский

Процитировано

0

Novel Strategies for Designing Climate‐Smart Crops to Ensure Sustainable Agriculture and Future Food Security DOI Creative Commons
Ali Raza, Tushar Khare, Xinyue Zhang

и другие.

Journal of Sustainable Agriculture and Environment, Год журнала: 2025, Номер 4(2)

Опубликована: Март 31, 2025

ABSTRACT To fulfil food and nutritional demand for nine billion people by the mid‐21st century, global production must increase 60% regardless of challenges such as environmental pollution, water scarcity land degradation. Climate change exacerbates frequency intensity biotic abiotic stresses, which, in turn, severely compromise crop yields, jeopardize supply, deteriorate sustainable development goals achieving safety, limit climate‐smart production. Current consumption practices negatively influence environment, posing a major threat to ecosystem human health. Addressing these critical issues achieve agriculture necessitates designing future crops employing cutting‐edge breeding strategies enhanced productivity with minimal footprints. This endeavour requires comprehensive understanding plant stress adaptation, signalling pathways mitigation mechanisms. In this review, we first explain diverse impacts ongoing climate events on Subsequently, outline various tackle change, including agronomic practices, advanced technologies physiological molecular mechanisms tolerance. We also discuss engineering superior tolerance disease resistance nurturing healthy microbial partnerships between plants soil ensure nutrition security current populations amidst mounting challenges.

Язык: Английский

Процитировано

0

The rhizobacterial Priestia megaterium strain SH-19 mitigates the hazardous effects of heat stress via an endogenous secondary metabolite elucidation network and molecular regulation signalling DOI Creative Commons
Shifa Shaffique, Anis Ali Shah,

Odongkara Peter

и другие.

BMC Plant Biology, Год журнала: 2024, Номер 24(1)

Опубликована: Сен. 4, 2024

Язык: Английский

Процитировано

3

Beyond Correlation: Understanding the Causal Link Between Microbiome and Plant Health DOI Creative Commons
Oluwaseyi Samuel Olanrewaju, Bernard R. Glick, Olubukola Oluranti Babalola

и другие.

Heliyon, Год журнала: 2024, Номер 10(23), С. e40517 - e40517

Опубликована: Ноя. 19, 2024

Язык: Английский

Процитировано

3

Integrated Metabolomics and Transcriptomic Analysis of Cellular Stress Responses: Elucidating Metabolic Pathways and Regulatory Networks DOI

Gul Rukh Askary,

Zafar U. Khan,

Syeda Samira Azim

и другие.

Indus journal of bioscience research., Год журнала: 2024, Номер 2(2), С. 1191 - 1197

Опубликована: Дек. 22, 2024

Cellular stress responses are vital for maintaining homeostasis and enabling adaptation to environmental physiological challenges. This study employed an integrated metabolomic transcriptomic approach investigate the regulatory networks metabolic pathways underlying these responses. Conducted between July 2023 December 2024 in Karachi, Pakistan, research utilized cutting-edge technologies, including real-time PCR, high-performance liquid chromatography (HPLC), gas chromatography-mass spectrometry (GC-MS), next-generation sequencing (NGS). Biological samples representing various conditions, such as oxidative stress, nutrient deprivation, stressors, were collected analyzed under strict ethical protocols. RNA (RNA-Seq) revealed 1,250 differentially expressed genes (DEGs), with notable upregulation of GPX1 SOD2, indicating enhanced detoxification stress. Concurrently, profiling identified significant alterations metabolites, elevated glutathione, lactate, proline, highlighting adaptive shifts glycolysis, reactive oxygen species (ROS) detoxification, osmoprotection. Integrative analysis using Weighted Gene Co-expression Network Analysis (WGCNA) pinpointed key hubs, HIF1A succinate, central nodes stress-specific networks. comprehensive multi-omics provided actionable insights into molecular mechanisms cellular The findings hold translational potential agriculture medicine, particularly enhancing crop resilience developing targeted therapies stress-related disorders. Despite limitations vitro models, this underscores value multi-layered omics analyses elucidating lays groundwork future integrative

Язык: Английский

Процитировано

0