The right tool for the job: Chemical biology and microbiome science

Cell chemical biology, Год журнала: 2025, Номер 32(1), С. 83 - 97

Опубликована: Янв. 1, 2025

Язык: Английский

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Glycoengineering with neuraminic acid analogs to label lipooligosaccharides and detect native sialyltransferase activity in gram-negative bacteria

Glycobiology, Год журнала: 2024, Номер 34(10)

Опубликована: Авг. 30, 2024

Lipooligosaccharides are the most abundant cell surface glycoconjugates on outer membrane of Gram-negative bacteria. They play important roles in host-microbe interactions. Certain pathogenic bacteria cap their lipooligosaccharides with sialic acid, N-acetylneuraminic acid (Neu5Ac), to mimic host glycans that among others protects these from recognition by hosts immune system. This process molecular mimicry is not fully understood and remains under investigated. To explore functional role acid-capped at level, it have tools readily available for detection manipulation both Neu5Ac involved sialyltransferases, preferably live We shown native sialyltransferases some can incorporate extracellular unnatural nucleotides onto lipooligosaccharides. here report expanded use bacterial neuraminic acids analogs a reporter group into variety show this approach offers quick strategy screen expression ability exogenous CMP-Neu5Ac decorate glycoconjugates. For selected we also complements two other glycoengineering techniques, Metabolic Oligosaccharide Engineering Selective Exo-Enzymatic Labeling, together they provide modify, label, detect visualize sialylation

Язык: Английский

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Glycoengineering with neuraminic acid analogs to label lipooligosaccharides and detect native sialyltransferase activity in Gram-negative bacteria

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Июль 6, 2024

Abstract Lipooligosaccharides (LOS) are the most abundant cell surface glycoconjugates on outer membrane of Gram-negative bacteria. They play important roles in host-microbe interactions. Certain pathogenic bacteria cap their LOS with sialic acid, N -acetylneuraminic acid (Neu5Ac), to mimic host glycans that among others protects these from recognition by hosts immune system. This process molecular mimicry is not fully understood and remains under investigated. To explore functional role acid-capped lipooligosaccharides at level, it have tools readily available for detection manipulation both Neu5Ac involved sialyltransferases, preferably live We shown native sialyltransferases some can incorporate extracellular unnatural nucleotides onto LOS. here report expanded use bacterial neuraminic acids analogs a reporter group into variety show this approach offers quick strategy screen expression ability exogenous CMP-Neu5Ac decorate glycoconjugates. For selected we also complements two other glycoengineering techniques, Metabolic Oligosaccharide Engineering (MOE) Selective Exo-Enzymatic Labelling (SEEL), together they provide modify, label, detect visualize sialylation

Язык: Английский

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Discovering microbiota functions via chemical probe incorporation for targeted sequencing

Current Opinion in Chemical Biology, Год журнала: 2024, Номер 84, С. 102551 - 102551

Опубликована: Ноя. 30, 2024

Язык: Английский

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