S100A13-driven interaction between pancreatic adenocarcinoma cells and cancer-associated fibroblasts promotes tumor progression through calcium signaling DOI Creative Commons

Liu-Yuan Xia,

Xin Guo,

Dong Lu

и другие.

Cell Communication and Signaling, Год журнала: 2025, Номер 23(1)

Опубликована: Янв. 28, 2025

Cancer-associated fibroblasts (CAFs) are key components of the pancreatic adenocarcinoma (PAAD) tumor microenvironment (TME), where they promote progression and metastasis through immunosuppressive functions. Although significant progress has been made in understanding crosstalk between cancer cells CAFs, many underlying mechanisms remain unclear. Recent studies have highlighted importance calcium signaling enhancing interactions surrounding stroma, with S100 family proteins serving as important regulators. While roles some extensively studied, others, such S100A13, less well understood. Bioinformatic analysis was employed to predict pathogenic potential CAFs S100A13. Stable S100A13 knockdown were generated using a short hairpin RNA system. Cellular viability apoptosis rates evaluated CCK-8 flow cytometry tests, respectively. Additionally, wound healing migration assays conducted assess invasive metastatic capabilities. Transcriptome identify differential gene expression associated pathways PAAD derived from an indirect culture Furthermore, protumoral role further verified both 3D bioprinting cell line-based xenograft models. In this study, we identified strong association calcium-binding protein, PAAD. Gene revealed that highly expressed correlated poor prognosis. Knockdown reduced cells. addition, depletion impaired motility within TME. silencing markedly slowed spheroids Balb/c nude mice. Together, our findings underscore critical CAFs-derived suggest targeting may offer promising therapeutic strategy for

Язык: Английский

S100A13-driven interaction between pancreatic adenocarcinoma cells and cancer-associated fibroblasts promotes tumor progression through calcium signaling DOI Creative Commons

Liu-Yuan Xia,

Xin Guo,

Dong Lu

и другие.

Cell Communication and Signaling, Год журнала: 2025, Номер 23(1)

Опубликована: Янв. 28, 2025

Cancer-associated fibroblasts (CAFs) are key components of the pancreatic adenocarcinoma (PAAD) tumor microenvironment (TME), where they promote progression and metastasis through immunosuppressive functions. Although significant progress has been made in understanding crosstalk between cancer cells CAFs, many underlying mechanisms remain unclear. Recent studies have highlighted importance calcium signaling enhancing interactions surrounding stroma, with S100 family proteins serving as important regulators. While roles some extensively studied, others, such S100A13, less well understood. Bioinformatic analysis was employed to predict pathogenic potential CAFs S100A13. Stable S100A13 knockdown were generated using a short hairpin RNA system. Cellular viability apoptosis rates evaluated CCK-8 flow cytometry tests, respectively. Additionally, wound healing migration assays conducted assess invasive metastatic capabilities. Transcriptome identify differential gene expression associated pathways PAAD derived from an indirect culture Furthermore, protumoral role further verified both 3D bioprinting cell line-based xenograft models. In this study, we identified strong association calcium-binding protein, PAAD. Gene revealed that highly expressed correlated poor prognosis. Knockdown reduced cells. addition, depletion impaired motility within TME. silencing markedly slowed spheroids Balb/c nude mice. Together, our findings underscore critical CAFs-derived suggest targeting may offer promising therapeutic strategy for

Язык: Английский

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