Unveiling the therapeutic potential of cabozantinib-loaded poly D,L-lactic-co-glycolic acid and polysarcosine nanoparticles in inducing apoptosis and cytotoxicity in human HepG2 hepatocellular carcinoma cell lines and in vivo anti-tumor activity in SCID female mice DOI Creative Commons
Sankha Bhattacharya, Vipan K. Parihar, Bhupendra G. Prajapati

и другие.

Frontiers in Oncology, Год журнала: 2023, Номер 13

Опубликована: Фев. 15, 2023

The study aimed to develop a nano-based drug delivery system for the treatment of hepatocellular carcinoma (HCC), type liver cancer that accounts 90% all malignancies. focused on use cabozantinib (CNB), potent multikinase inhibitor targets VEGF receptor 2, as chemotherapeutic drug. We developed CNB-loaded nanoparticles made from Poly D, L-lactic-co-glycolic acid, and Polysarcosine (CNB-PLGA-PSar-NPs) in human HepG2 cell lines.By O/W solvent evaporation method, polymeric were prepared. various techniques, such photon correlation spectroscopy, scanning electron microscopy, transmission microscopy used, determine formulation's particle size, zeta potential, morphology. SYBR Green/ROX qPCR Master Mix RT-PCR equipment used measure line tissue mRNA expression MTT assay test cytotoxicity. Cell cycle arrest analysis, annexin V assay, ZE5 Analyzer apoptosis also performed.The results showed diameters 192.0 ± 3.67 nm with 0.128 PDI -24.18 3.34 mV potential. antiproliferative proapoptotic effects CNB-PLGA-PSar-NPs evaluated using flow cytometry (FCM). IC50 value was 45.67 µg/mL, 34.73 21.56 µg/mL 24, 48, 72 h, respectively. found 11.20% 36.77% CNB-PLGA-PSar-NPs-treated cells apoptotic at 60 80 respectively, suggesting effective inducing cells. It can conclude that, inhibit kill them by upregulating tumour suppressor genes MT1F, MT1X, downregulating MTTP, APOA4. Further vivo antitumor activity well reported SCID female mice.Overall, this suggests are promising HCC, further research is needed investigate their potential clinical treatment.

Язык: Английский

Unveiling the therapeutic potential of cabozantinib-loaded poly D,L-lactic-co-glycolic acid and polysarcosine nanoparticles in inducing apoptosis and cytotoxicity in human HepG2 hepatocellular carcinoma cell lines and in vivo anti-tumor activity in SCID female mice DOI Creative Commons
Sankha Bhattacharya, Vipan K. Parihar, Bhupendra G. Prajapati

и другие.

Frontiers in Oncology, Год журнала: 2023, Номер 13

Опубликована: Фев. 15, 2023

The study aimed to develop a nano-based drug delivery system for the treatment of hepatocellular carcinoma (HCC), type liver cancer that accounts 90% all malignancies. focused on use cabozantinib (CNB), potent multikinase inhibitor targets VEGF receptor 2, as chemotherapeutic drug. We developed CNB-loaded nanoparticles made from Poly D, L-lactic-co-glycolic acid, and Polysarcosine (CNB-PLGA-PSar-NPs) in human HepG2 cell lines.By O/W solvent evaporation method, polymeric were prepared. various techniques, such photon correlation spectroscopy, scanning electron microscopy, transmission microscopy used, determine formulation's particle size, zeta potential, morphology. SYBR Green/ROX qPCR Master Mix RT-PCR equipment used measure line tissue mRNA expression MTT assay test cytotoxicity. Cell cycle arrest analysis, annexin V assay, ZE5 Analyzer apoptosis also performed.The results showed diameters 192.0 ± 3.67 nm with 0.128 PDI -24.18 3.34 mV potential. antiproliferative proapoptotic effects CNB-PLGA-PSar-NPs evaluated using flow cytometry (FCM). IC50 value was 45.67 µg/mL, 34.73 21.56 µg/mL 24, 48, 72 h, respectively. found 11.20% 36.77% CNB-PLGA-PSar-NPs-treated cells apoptotic at 60 80 respectively, suggesting effective inducing cells. It can conclude that, inhibit kill them by upregulating tumour suppressor genes MT1F, MT1X, downregulating MTTP, APOA4. Further vivo antitumor activity well reported SCID female mice.Overall, this suggests are promising HCC, further research is needed investigate their potential clinical treatment.

Язык: Английский

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