Analytical Chemistry,
Год журнала:
2024,
Номер
96(10), С. 4129 - 4137
Опубликована: Март 4, 2024
Long-term
continuous
imaging
of
endogenous
HClO
burst
is
great
importance
for
the
elucidation
various
physiological
or
pathological
processes.
However,
most
currently
reported
probes
have
failed
to
achieve
this
goal
due
their
insufficient
photobleaching
resistance
under
a
laser
source.
Herein,
highly
stable
ratiometric
probe,
HFTC-HClO
1,
which
capable
continuously
monitoring
over
long
period
time,
has
been
judiciously
developed.
Briefly,
de
novo
development
1
mainly
involved
three
main
steps:
(1)
novel
coumarins
(HFTC
1–5)
were
designed
and
synthesized;
(2)
scaffold,
HFTC
3,
was
selected
through
dye
screening
cell
validation;
(3)
based
on
candidate
constructed,
finally
its
superior
sensing
properties
toward
HClO.
Furthermore,
can
quantitatively
measure
levels
in
real
samples
with
excellent
recovery
(>90.4%),
use
1-coated
test
strips
qualitative
analysis
also
achieved.
In
addition,
application
long-term
intracellular
successfully
demonstrated.
Significantly,
able
visualize
generated
rheumatoid
arthritis
mouse
model.
ABSTRACT
Hypochlorous
acid
(HClO)
is
released
by
immune
cells
in
the
system,
and
it
helps
body
fight
off
infections
inflammation
killing
bacteria,
viruses,
other
pathogens.
However,
tissue
damage
or
apoptosis
may
also
be
induced
excess
HClO.
On
this
basis,
we
designed
probe
TPE‐NS
choosing
tetraphenylethylene
(TPE)
as
luminescent
unit
dimethylthiocarbamoyl
chloride
recognition
site.
By
Gaussian's
transition
state
calculations,
HClO
will
cut
photoinduced
electron
transfer
(PET)
effect
of
hydrolysis
reaction,
thus
emitting
strong
fluorescence.
has
rapid
excellent
specificity
for
HClO,
limit
detection
7.27
μM.
Finally,
was
successfully
used
visualization
endogenous
exogenous
cell
experiments.
Analytical Chemistry,
Год журнала:
2024,
Номер
97(1), С. 220 - 228
Опубликована: Дек. 31, 2024
Developing
highly
specific
hypochlorite
(HClO)
ratiometric
fluorescent
probes
for
elucidating
drug-induced
liver
injury
(DILI)
and
related
biological
issues
is
of
great
significance.
Phenothiazine-derived
coumarin
(PTZC)
fluorophores
have
been
shown
to
exhibit
high
specificity
in
response
HClO;
however,
such
emit
dim
fluorescence
aqueous
media
due
the
intrinsic
twisted
intramolecular
charge
transfer
(TICT)
effect,
resulting
only
a
turn-on
mode
HClO.
In
this
work,
we
present
molecular
design
strategy
suppressing
TICT
formation
by
inducing
PTZC
form
nanoaggregates
HClO
probe
design.
The
PBFF
developed
based
on
can
considerable
inhibit
without
suffering
from
aggregation-caused
quenching
thus
allowing
it
intense
(Φf
=
0.20).
Importantly,
specifically
sensitively
(LOD
15.7
nM)
respond
HClO,
showing
distinct
changes.
Further
experimental
results
show
that
monitor
situ
DILI
progression
through
HClO-induced
ratio
This
molecule
aggregation
will
advance
development
future.
Advanced Functional Materials,
Год журнала:
2024,
Номер
unknown
Опубликована: Авг. 29, 2024
Abstract
pH
is
spatiotemporal
variable
dynamically
in
physiological
and
pathological
processes,
the
tiny
changes
can
result
completely
different
processes.
However,
major
drawbacks
of
conventional
pH‐activatable
probes
are
their
large
acid‐base
transition
width
challenge
to
adjust
pKa
suitable
for
various
application.
In
response
these
issues,
a
novel
approach
proposed
realize
sensitive
general
strategy
construct
multicolor
pKa‐tunable
xanthene‐based
platform.
probes,
narrowest
range
just
1
units,
achieved
by
introducing
electron‐rich
pyridine
as
ingenious
proton
catcher.
Especially,
Si‐4Py
owns
near‐infrared
(NIR)
signal
5.56,
which
successfully
used
rapid
high‐contrast
imaging
distal
metastatic
tumors.
This
has
significant
implications
guiding
surgeons
removal
tumors
ultimately
improve
patient
outcomes.
Additional,
sustainably
innovative
this
work
serve
effective
reference
probe
development.
