Foods,
Год журнала:
2024,
Номер
13(23), С. 3816 - 3816
Опубликована: Ноя. 26, 2024
The
self-quenching
fluorogenic
probe
facilitates
precise
identification
of
LAMP
(loop-mediated
isothermal
amplification)
amplicons,
unaffected
by
non-specific
products
resulting
from
primer
dimers.
However,
low
quenching
efficiency
surrounding
nucleobases
leads
to
high
background
signal,
posing
significant
challenges
for
visual
inspection
with
the
naked
eye.
present
study
aims
identify
an
oligonucleotide
sequence
that
is
complementary
probe,
and
employ
fluorescence
super-quenching
mechanism
double-stranded
DNA
establish
a
visualization
system
assay.
results
indicated
incorporation
fully
could
significantly
reduce
system’s
(p
<
0.05).
When
melting
temperature
exceeds
room
temperature,
truncating
3′
end
does
not
compromise
probe’s
efficiency.
system,
using
10–13-base
loop
primer-based
effectively
minimize
yield
straightforward
post-reaction.
Applied
rainbow
trout
Atlantic
salmon
detection,
detected
1
pg
in
closed-tube
format.
In
conclusion,
suitable
can
probe.
Employing
this
alongside
develop
low-background
demonstrates
great
potential
successful
detection
holds
considerable
promotional
merit.
The Analyst,
Год журнала:
2024,
Номер
149(16), С. 4095 - 4115
Опубликована: Янв. 1, 2024
Carbon
dots
(CDs),
including
carbon
quantum
dots,
graphene
nanodots,
and
polymer
have
gained
significant
attention
due
to
their
unique
structural
fluorescence
characteristics.
This
review
provides
a
comprehensive
overview
of
the
classification,
characteristics,
properties
CDs,
followed
by
an
exploration
various
sensing
mechanisms
applications
in
gene
detection,
nucleolus
imaging,
delivery.
Furthermore,
functionalization
CDs
with
diverse
surface
ligand
molecules,
dye
nucleic
acid
probes,
metal
derivatives,
for
sensitive
detection
is
systematically
examined.
Fluorescence
imaging
cell
plays
vital
role
examining
intracellular
processes
dynamics
subcellular
structures.
By
analyzing
mechanism
structure-function
relationships
inherent
targeting
abilities
lines
been
discussed.
Additionally,
challenges
such
as
insufficient
organelle
specificity
inconsistent
underlying
also
highlighted.
The
physical
chemical
particularly
strong
affinity
toward
deoxyribonucleic
(DNA),
spurred
interest
delivery
applications.
use
nuclear-targeting
peptides,
polymers,
ligands
conjunction
improved
reviewed.
Through
analysis,
aims
contribute
deeper
understanding
potential
associated
biomedical
In
this
study,
curcumin
which
is
a
biodegradable,
non-toxic,
and
natural
colorant,
served
as
pH
indicator
to
visually
identify
the
LAMP-amplified
Staphylococcus
aureus
(
S.
)
Streptococcus
pneumoniae
).
Journal of Agricultural and Food Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Март 24, 2025
Nucleic
acid
testing
is
increasingly
being
utilized
in
the
detection
of
foodborne
pathogens.
The
implementation
fully
automated
and
enclosed
nucleic
systems
has
potential
to
significantly
reduce
manual
labor
time
for
operators.
Therefore,
development
such
become
a
focal
point
current
research.
In
this
study,
focus
on
addressing
how
achieve
at
low
cost.
research
primarily
centered
design
key
functional
modules
required
process
including
extraction,
heating,
optical
detection.
does
not
require
supervision
or
participation
professional
technical
personnel,
thereby
reducing
usage
By
simply
adding
sample,
it
can
simultaneously
screen
analyze
different
types
pathogens,
making
advantageous
analysis
complex
samples.
manufacturing
device
are
cost-effective
highly
applicable.
adjusting
reaction
program,
compatibility
with
various
amplification
reactions,
demonstrating
its
broad
application
prospects
field
Cronobacter
sakazakii
analysis.
Critical Reviews in Analytical Chemistry,
Год журнала:
2025,
Номер
unknown, С. 1 - 29
Опубликована: Март 27, 2025
The
urgent
need
for
sensitive,
rapid,
and
reliable
diagnostic
methodologies
to
control
prevent
life-threatening
pandemic
infectious
disease,
such
as
COVID-19,
remains
a
critical
priority.
Timely
on-site
detection
of
viral
pathogens
is
essential
effective
disease
management
mitigation
societal
disruptions.
Recent
advancements
in
optical
methods
have
positioned
them
at
the
forefront
healthcare
diagnostics,
offering
high
sensitivity
specificity
viable
alternatives
conventional
techniques
Polymerase
Chain
Reaction
(PCR),
which
often
suffer
from
time
delays
limited
accessibility
resource-constrained
environments.
This
review
elucidates
potential
various
techniques,
highlighting
their
advantages
over
traditional
methods.
It
encompasses
range
modalities,
including
fluorescence-based
approaches,
Raman
spectroscopy
(RS),
Plasmonic
(e.g.,
surface
plasmon
resonance
(SPR),
localized
SPR,
(LSPR),
surface-enhanced
(SERS),
fluorescence
(SEF)),
super
resolution
microscopies
(SRMs),
attenuated
total
reflectance-Fourier
transform
infrared
(ATR-FTIR),
integrated
platforms
waveguides
molecularly
imprinted
polymer
(MIP)-based
biosensors.
Additionally,
evolution
novel
biosensors,
particularly
5th
6th
generation
challenges
related
these
technologies
were
discussed.
studies
reviewed
aims
advance
development
portable,
specific,
cost-effective
point-of-care
(POC)
devices
rapid
pathogens.
Analytical Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Март 30, 2025
The
highly
sensitive
and
rapid
imaging
of
miRNA
in
living
cells
promises
to
advance
our
understanding
diseases
promote
their
diagnosis
treatment.
To
enhance
the
sensitivity
imaging,
a
series
cascade
signal
amplification
strategies
based
on
enzyme-free
methods
have
been
developed.
However,
these
cascaded
involve
complex
designs
multiple
mechanisms,
leading
potential
side
effects.
Herein,
we
developed
novel
hairpins@ZIF-8
nanosystem
by
rationally
integrating
ZIF-8
with
self-feedback
DNAzyme
circuit
(CSDC),
achieving
cells.
facilitates
efficient
transfection
nucleic
acid
probes
into
provides
necessary
cofactor
ions
for
CSDC.
CSDC
possesses
exponential
solely
mechanism.
Benefiting
from
efficiency,
exhibited
higher
than
traditional
DNAzyme-based
amplification,
detection
limit
2.28
fM,
approximately
105
times
more
amplification.
This
demonstrated
strong
practical
application
capabilities,
effectively
reflecting
fluctuations
intracellular
levels
successfully
distinguishing
between
normal
tumor
expression
differences.
It
could
also
be
applied
vivo
imaging.
proposed
strategy
is
anticipated
pave
way
innovative
approaches
serve
as
vital
instrument
miRNA-related
research,
diagnosis,
