Fluorescence
imaging
in
the
second
near-infrared
region
(NIR-II,
1000-1700
nm)
has
garnered
considerable
attention
for
displaying
biological
information
of
deep
tissues.
However,
lack
biocompatible
contrast
agents
with
bright
NIR-II
emission
hampered
precise
clinical
application
tissue
imaging.
Here,
a
lipophilic
enhancement
strategy
employing
donor-acceptor-donor
(D-A-D)
molecules,
introducing
long
alkoxy
chains
and
quaternary
ammonium
salts
development
highly
water-soluble
fluorophores
(BBTD-2C-N),
is
described.
Notably,
liposome-encapsulated
BBTD-2C-N
nanoparticles
(B-2C-N/DMPC)
aqueous
solution
exhibit
1.8-fold
increase
fluorescence
brightness
compared
to
free
methanol.
Avoidance
aggregation-caused
quenching
effect
enhanced
are
attributed
significantly
attenuated
π-π
stacking
interactions
maintained
monodisperses
hydrophobic
liposome
shell.
Moreover,
demonstrates
superior
performance
visualizing
lipid
droplet-rich
HeLa
cells
vitro,
as
well
monitoring
adipose
fatty
liver
vivo.
This
study
reveals
new
avenue
vivo
Inorganic Chemistry,
Год журнала:
2024,
Номер
63(28), С. 13059 - 13067
Опубликована: Июнь 28, 2024
Type
I
photosensitizers
offer
an
advantage
in
photodynamic
therapy
(PDT)
due
to
their
diminished
reliance
on
oxygen
levels,
thus
circumventing
the
challenge
of
hypoxia
commonly
encountered
PDT.
In
this
study,
we
present
synthesis
and
comprehensive
characterization
a
novel
type
photosensitizer
derived
from
cyclometalated
Ir(III)-rhodamine
complex.
Remarkably,
complex
exhibits
shift
absorption
fluorescence,
transitioning
"off"
"on"
states
aprotic
protic
solvents,
respectively,
contrary
initial
expectations.
Upon
exposure
light,
demonstrates
effective
generation
O2–
·OH
radicals
via
mechanism.
Additionally,
it
notable
antibacterial
activity
against
both
Gram-positive
Gram-negative
bacteria,
demonstrated
through
vitro
vivo
experiments.
This
research
offers
valuable
insights
for
development
photosensitizers.
Analytical Chemistry,
Год журнала:
2025,
Номер
97(1), С. 1003 - 1009
Опубликована: Янв. 1, 2025
At
present,
some
progress
has
been
made
in
developing
NIR
light-responsive
free
radical
generators.
However,
the
efficacy
of
theranostics
continues
to
be
hindered
by
tumor-associated
inflammatory
reactions.
Hence,
fulfilling
situ
release
radicals
upon
light
excitation
specifically
activated
inflammation
microenvironment
would
an
ideal
strategy
for
efficient
eradication
and
tumor
suppression
but
remains
a
challenge.
Herein,
SO2
(overexpressed
reactive
sulfur
species
inflamed
site)-stimulated
phototheranostic
agent
(CVS)
is
successfully
developed.
Through
specific
response
both
endogenous
exogenous
with
low
LOD
(31.7
nM),
CVS
demonstrates
"switch
on"
two-photon
activity
as
well
OH·
generation.
Remarkably,
CVS-treated
H22-tumor-bearing
mice,
light-activated
accurate
are
accomplished.
This
platform
not
only
facilitates
quantification
during
also
renders
it
potent
antihypoxic
agent.
Inorganic Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 11, 2025
Acute
bacterial
skin
and
structure
infections
(ABSSSIs)
pose
significant
global
health
challenges,
exacerbated
by
rising
antibiotic
resistance.
Antibacterial
photodynamic
therapy
(APDT)
has
emerged
as
a
promising
strategy
to
combat
these
utilizing
photosensitizer
(PS)
that
generates
reactive
oxygen
species
(ROS)
upon
light
activation.
However,
the
limited
tissue
penetration
of
conventional
organic
PSs,
which
primarily
absorb
in
UV-vis
spectra,
hindered
their
therapeutic
potential
for
deeper
infections.
Herein,
we
introduce
novel
iridium(III)-cyanine
complex
(Ir-cy)
with
strong
near-infrared
(NIR)
absorption
at
814
nm
(up
101
red-shifted
from
previous
reports),
specifically
designed
enhance
APDT.
Under
808
laser
irradiation,
Ir-cy
demonstrated
substantial
ROS
generation
capacity,
achieving
approximately
70%
reduction
Staphylococcus
aureus
(S.
aureus)
colonies
depth
7.2
mm
within
simulated
model.
Comprehensive
vitro
vivo
evaluations
further
confirmed
its
potent
antibacterial
efficacy
against
S.
while
maintaining
excellent
biocompatibility.
These
findings
highlight
highly
effective
NIR-active
PS,
paving
way
advanced
strategies
targeting
deep-tissue
ABSSSIs
through
optimized
Bioconjugate Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Март 31, 2025
The
understanding
of
diseases
such
as
cancer
and
Alzheimer's,
along
with
natural
aging
processes,
heavily
relies
on
the
study
mitochondrial
function.
Optical
techniques
like
fluorescence
imaging
microscopy
are
pivotal
for
this
purpose,
enabling
precise
mapping
subcellular
structures,
including
mitochondria.
In
study,
we
explored
TAPY
(triarylpyridinium)
cations,
a
novel
family
carriers
resembling
well-known
triphenylphosphonium
cation
(TPP).
Six
TAPY-bodipy
(BDP)
dyads
were
prepared
chemically
characterized.
Confocal
Laser
Scanning
Microscopy
(CLSM)
studies
demonstrated
that
systems
delivered
selectively
to
mitochondria
cells
(MCF-7,
A549,
HT-29).
Remarkably,
these
did
not
target
normal
(HEK-293,
HMEC-1),
suggesting
their
potential
use
in
distinguishing
cancerous
from
healthy
ones.
A
model
compound
comprised
same
bodipy
cargo
but
attached
TPP
was
also
synthesized
tested.
Notably,
preliminary
comparative
assays
MCF-7
cells,
dyad
TAPY(OMe)-BDP
outperformed
derivative
imaging,
achieving
twice
final
intensity.
chemical
diversity
achievable
cations
is
considerable,
many
derivatives
being
accessible
starting
readily
available
commercial
products.
This
implies
that,
based
strategy
outlined
carefully
optimized
targeted
delivery
could
potentially
be
developed
future
alternatives
or
complements
TPP,
present
work
acting
proof
concept.
