Enhancing Phosphorus Release from Sewage Sludge in Anaerobic Digestion via Thermal Hydrolysis Pretreatment: Insights from Phosphorus Speciation and Molecular Biological Pathways

Environmental Science & Technology, Год журнала: 2024, Номер 58(24), С. 10828 - 10838

Опубликована: Июнь 3, 2024

This study explores the mechanisms enhancing phosphorus (P) release from sludge in anaerobic digestion (AD) with thermal hydrolysis pretreatment (THP) using sequential chemical extraction, X-ray absorption near-edge structure spectroscopy (XANES), 31P NMR, and multiomics. THP-treated notably increased liquid-phase P by 53.8% over 3 days compared to sewage (SS), identifying solid-phase Fe–P as primary source. The THP+AD also provided a higher abundance of bacteria that contributed through multiple pathways (MPRPB), whereas SS+AD enriched some microbial species single pathway. Moreover, co-occurrence network analysis underlined pivotal role P-releasing THP+AD, 8 out 16 keystones being P-releasers. Among 63 screened genes were related transformations release, poly beta-hydroxybutyrate (PHB) synthesis associated polyphosphate bacteria-mediated more abundant than SS+AD. Furthermore, upregulation involved methyl phosphonate metabolism enhanced methane production potential AD process. These findings suggested MPRPB indeed main contributors enrichment process their capability for liberation.

Язык: Английский

---

Salinity stress results in ammonium and nitrite accumulation during the elemental sulfur-driven autotrophic denitrification process

Frontiers in Microbiology, Год журнала: 2024, Номер 15

Опубликована: Фев. 14, 2024

In this study, we investigated the effects of salinity on elemental sulfur-driven autotrophic denitrification (SAD) efficiency, and microbial communities. The results revealed that when was ≤6 g/L, nitrate removal efficiency in SAD increased with increasing reaching 95.53% at 6 g/L salinity. Above salt concentration, performance gradually decreased, decreased to 33.63% 25 Approximately 5 mg/L hazardous nitrite detectable 15 salinity, but accompanied by generation ammonium. When ≥15 abundance salt-tolerant microorganisms, Thiobacillus Sulfurimonas , increased, while other species decreased. This study provides support for practical application saline wastewater.

Язык: Английский

---

A Novel AOA-SNDPRr Process for Simultaneous Wastewater Treatment, Phosphorus Recovery, and In Situ Sludge Reduction with Ultralong Sludge Retention Time

ACS ES&T Engineering, Год журнала: 2025, Номер unknown

Опубликована: Янв. 4, 2025

Successive side-stream phosphorus recovery challenges the stable enhanced biological (P) removal process due to stripping of P from sludge. In this study, a novel anaerobic/oxic/anoxic simultaneous nitrification, denitrification, and integrating (AOA-SNDPRr) was proposed operated under ultralong sludge retention time (SRT, 120–140 d). Results showed that effective carbon, nitrogen, obtained. High contents within (15–30 mg P/g MLSS) were kept even after an 80 day continuous extraction, thus supporting considerable anaerobic concentrations (10–30 mg/L). Up 81.60% influent harvested, in situ reduction by 63.2% with low observed yield (Yobs) 0.015 g MLSS/g chemical oxygen demand 50% extraction ratio. Interestingly, long-time operation witnessed spontaneous granulation. Abundant extracellular polymeric substances (EPS), particularly viscous polysaccharides (PS), stimulated. A 16S rDNA sequence demonstrated overwhelmingly dominated Candidatus_Competibacter soared 20.18%–25.91% without deteriorating removal. Negligible impact on functional genes for poly-P/Gly synthesis decomposition found. Overall, AOA-SNDPRr long SRT proved be sustainable approach targeting three goals reliable wastewater treatment, efficient recovery, dramatic minimization.

Язык: Английский

---

Achieving synchronous nitrogen and phosphorus removal by aerobic enrichment of electrotrophic/heterotrophic bacteria and denitrifying polyphosphate-accumulating organisms in repeatedly oxygen-rich microbial fuel cells

Bioresource Technology, Год журнала: 2025, Номер unknown, С. 132297 - 132297

Опубликована: Фев. 1, 2025

Язык: Английский

---

Genome-resolved metagenomic analysis reveals a novel denitrifier with truncated nitrite reduction pathway from the genus SC-I-84

Water Research, Год журнала: 2025, Номер 282, С. 123598 - 123598

Опубликована: Апрель 16, 2025

Язык: Английский

---

The advanced treatment of textile printing and dyeing wastewater by hydrodynamic cavitation and ozone: Degradation, mechanism, and transformation of dissolved organic matter

Environmental Research, Год журнала: 2022, Номер 215, С. 114300 - 114300

Опубликована: Сен. 9, 2022

Язык: Английский

---

Comparing with oxygen, nitrate simplifies microbial community assembly and improves function as an electron acceptor in wastewater treatment

Environmental Pollution, Год журнала: 2022, Номер 314, С. 120243 - 120243

Опубликована: Сен. 22, 2022

Язык: Английский

---

Nitrite-resistance mechanisms on wastewater treatment in denitrifying phosphorus removal process revealed by machine learning, co-occurrence, and metagenomics analysis

Environmental Pollution, Год журнала: 2023, Номер 327, С. 121549 - 121549

Опубликована: Апрель 3, 2023

Язык: Английский

---

Metagenomics, metatranscriptomics, and proteomics reveal the metabolic mechanism of biofilm sequencing batch reactor with higher phosphate enrichment capacity under low phosphorus load

Environmental Research, Год журнала: 2023, Номер 238, С. 117237 - 117237

Опубликована: Окт. 2, 2023

Язык: Английский

---

Enrichment denitrifying phosphorus-accumulating organisms in alternating anoxic-anaerobic/aerobic biofilter for advanced nitrogen and phosphorus removal from municipal wastewater

Journal of Water Process Engineering, Год журнала: 2023, Номер 55, С. 104089 - 104089

Опубликована: Авг. 1, 2023

Язык: Английский

---