Microbial Enzyme Systems in the Production of Second Generation Bioethanol DOI Open Access
Sanjeev Kumar Soni, Apurav Sharma,

Raman Soni

и другие.

Опубликована: Дек. 20, 2022

The primary contributor to global warming has been the careless usage of fossil fuels. Urbanization's threat depletion these resources made it necessary find alternatives due rising demand. Four different forms biofuels are now available and constitute a possible replacement for first generation is generated from edible portion biomass, second non-edible third algal fourth using molecular biology improve strain. Second extremely important because they derived such as agricultural agro-industrial wastes rich in cellulose, hemicellulose, pectin, starch impregnated with lignin, hydrolyzed after delignification by physio-chemical or biological pretreatments ligninases. There lot research on their production chemical enzymatic hydrolysis, but not economically viable comparison formation several inhibitors hydrolysis high cost enzymes. Furthermore, need multiple enzymes various types carbohydrates feedstocks makes process too expensive. This article examines involved bioethanol, highly acceptable biofuel.

Язык: Английский

A novel AA14 LPMO from Talaromyces rugulosus with bifunctional cellulolytic/hemicellulolytic activity boosted cellulose hydrolysis DOI Creative Commons
Kaixiang Chen, Xu Zhao, Peiyu Zhang

и другие.

Biotechnology for Biofuels and Bioproducts, Год журнала: 2024, Номер 17(1)

Опубликована: Фев. 23, 2024

Abstract Background The recently discovered Pc AA14A and B from white-rot basidiomycete Pycnoporus coccineus enriched our understanding of the oxidative degradation xylan in fungi, however, unusual mode action AA14 LPMOs has sparked controversy. substrate specificity functionality still remain enigmatic need further investigation. Results In this study, a novel LPMO was characterized ascomycete Talaromyces rugulosus . Tr broad with strong activity on pure amorphous cellulose xyloglucan. It could simultaneously oxidize cellulose, xyloglucan natural hemi/cellulosic such as fibrillated eucalyptus pulp, released native oxidized cello-oligosaccharides, xylo-oligosaccharides oligosaccharides substrate, but its cellulolytic/hemicellulolytic became weaker contents increase alkaline-extracted substrates. dual enables to possess profound boosting effect hydrolysis by cellulolytic enzymes. Structure modelling revealed that it exhibits relatively flat active-site surface similar surfaces AA9 quite distinct AA14B, despite is strongly clustered together LPMOs. Remarkable difference electrostatic potentials L2 L3 also observed among TrAA14A, AA14B Nc LPMO9F. We speculated unique feature substrate-binding might contribute AA14A. Conclusions extensive indicated distinctively different previously xylan-active or may play bifunctional enzyme decompose some specific network structures formed between hemicellulose plant cell walls. Our findings shed new insights into specificities biological functionalities LPMOs, will developing booster commercial cellulase cocktails efficiently break down hemicellulose-cellulose matrix lignocellulose.

Язык: Английский

Процитировано

1

Insight into the synergistic effects of surface microstructures and wall peristalsis on glucose production in a bionic microreactor DOI

Kai Lin,

Ao Xia, Yun Huang

и другие.

Chemical Engineering Journal, Год журнала: 2024, Номер 490, С. 151602 - 151602

Опубликована: Апрель 25, 2024

Язык: Английский

Процитировано

1

Advanced Technologies for Transforming Biomass to Biofuels DOI
Shehbaz Ali, Abida Rani, Sivasamy Sethupathy

и другие.

Studies in systems, decision and control, Год журнала: 2024, Номер unknown, С. 47 - 64

Опубликована: Янв. 1, 2024

Язык: Английский

Процитировано

0

Unveiling the secretome of Penicillium fuscoglaucum JAM-1 for efficient dual substrate degradation and waste valorization DOI Creative Commons
Namrata Joshi, Jasneet Grewal, Robert Stasiuk

и другие.

Biomass Conversion and Biorefinery, Год журнала: 2024, Номер unknown

Опубликована: Июнь 15, 2024

Abstract In the pursuit of cost-effective and superior enzymes crucial for efficient hydrolysis diverse lignocellulosic biomasses, filamentous fungi have emerged as key candidates bioprospecting endeavors. our exploration potent biomass degraders, we identified a strain Penicillium fuscoglaucum JAM-1, showcasing multipurpose hydrolase capabilities in its secretome. During fermentation, P. JAM-1 effectively utilized rapeseed cake (RSC), resulting improved enzymatic activities, including xylanase (612 U/gds), β-glucosidase (264 endoglucanase (102 FPase (21.3 exo-polygalacturonase (49.17 compared to pine sawdust (PSD). Secretome profiling revealed protein abundance totaling 435 120 proteins during RSC PSD utilization, respectively. The major component carbohydrate-active (CAZymes) consists cellulose-degrading proteins, endoglucanases (GH5, GH7), β-glucosidases (GH1, GH3, GH17), cellobiohydrolases (GH6, GH7). Correspondingly, hemicellulose-degrading were present, encompassing endo-1,4-xylanase (GH10), α/β-galactosidase (GH27, GH35, GH36), α/β-mannosidases (GH38, GH2, GH47, GH5), α- l -arabinofuranosidase (GH43, GH62, GH51, GH54) auxiliary activities enzymes, such AA9 (formerly known GH61) lytic polysaccharide monooxygenase (LPMO). Upon application fruit waste, crude enzyme demonstrated higher saccharification potential commercial cellulase (Cellic CTec2). Specifically, yielded 565 mg/g reducing sugar within 72 h, outperforming Cellic CTec2, which 352 under identical conditions. A comprehensive comparative analysis workings, secretome underscores producer, boost lignocellulose biodegradation. These findings highlight practical applications various industrial processes, suggesting role valuable candidate further exploitation biotechnological applications. Graphical abstract

Язык: Английский

Процитировано

0

Correlation Coefficients Effects of Fungal Secretome Cellulolytic Enzyme Cocktails and Synergistic Effects with Commercial Cellulase on Woody Biomass Degradation DOI
Cuiyi Liang,

Yu Long,

Wen Wang

и другие.

Опубликована: Янв. 1, 2024

Язык: Английский

Процитировано

0

Recent advancements and challenges in the lignocellulosic and algal biomass-based bioethanol production: a review DOI

Aparna Prasanthan,

R. Krishna Prasad,

Murali Balu

и другие.

Biofuels, Год журнала: 2024, Номер unknown, С. 1 - 20

Опубликована: Дек. 4, 2024

The sustained increase in global energy needs and environmental impacts from greenhouse gases emitted by burning non-renewable fuels has led to a focus on developing renewable sources. Bioethanol can meet future demands of nations. Lignocellulosic feedstock algal biomass are considered effective sources substrates for converting fermentable sugars into ethanol. structural configuration lignin-based cellulosic is modified using integrated pretreatment technologies, including physiochemical biological methods. hydrolysis cellulose with cellulase enzymes consists adsorption the surface, disintegration polymers simple sugar compounds, desorption cellulase. Soybean straw fermented Thermoanaerobacterium aotearoense at 55 °C 15 g/L total provided 0.34 g/g ethanol yield. Rice hydrolyzed 30 °C, 74 h, pH 4.5 yielded 0.44 macroalgae species Ulva rigida solid-state fermentation Saccharomyces cerevisiae 37 5, 3 h 330 mg/g glucose. microalga Chlorella vulgaris 45 6, 48 amylases 3.8 Different processes reduced include submerged, solid-state, very high-gravity fermentation.

Язык: Английский

Процитировано

0

Correlation and synergy between fungal secretome cellulolytic enzyme cocktails and commercial cellulase for woody biomass degradation DOI
Cuiyi Liang,

Yu Long,

Wen Wang

и другие.

Renewable Energy, Год журнала: 2024, Номер unknown, С. 122158 - 122158

Опубликована: Дек. 1, 2024

Язык: Английский

Процитировано

0

Effects of Penicillium EF-2 fermentation on Cyclocarya paliurus polysaccharide and preliminary mechanism exploration: Physicochemical, characterisation, in vitro immunomodulatory activity DOI
Gang Wang, Weidong Zhang,

Kai Cheng

и другие.

Food Bioscience, Год журнала: 2024, Номер unknown, С. 105777 - 105777

Опубликована: Дек. 1, 2024

Язык: Английский

Процитировано

0

Evaluation of Biological Pretreatment of Wormwood Rod Reies with White Rot Fungi for Preparation of Porous Carbon DOI Creative Commons

Wen Kong,

Shuhui Wang, Xinyu Zhang

и другие.

Journal of Fungi, Год журнала: 2022, Номер 9(1), С. 43 - 43

Опубликована: Дек. 28, 2022

In this work, the wormwood rod residues are pretreated with white rot fungi as precursor to preparing porous carbon following a simple carbonization and activation process (denoted herein FWRA sample). The sample possesses abundant hierarchical pores structure high specific surface area (1165.7 m2 g-1) large pore volume (1.02 cm3 g-1). As an electrode for supercapacitors, offers capacitance of 443.2 F g-1 at 0.5 A superb rate ability holding 270 100 in 6 M KOH electrolyte. corresponding symmetrical capacitor has cyclic stability low decay 0.4% after 20,000 cycles 5 1 Na2SO4 Moreover, measurements revealed that when used adsorbent, is ideal removing methyl orange (MO) from water, exhibiting superior adsorption 260.8 mg g-1. Therefore, study expected provide environmentally friendly technique generation value-added functional materials Chinese medicinal herbal residues, thus offering promising candidates broad application areas.

Язык: Английский

Процитировано

2

Microbial Enzyme Systems in the Production of Second Generation Bioethanol DOI Open Access
Sanjeev Kumar Soni, Apurav Sharma,

Raman Soni

и другие.

Опубликована: Дек. 20, 2022

The primary contributor to global warming has been the careless usage of fossil fuels. Urbanization's threat depletion these resources made it necessary find alternatives due rising demand. Four different forms biofuels are now available and constitute a possible replacement for first generation is generated from edible portion biomass, second non-edible third algal fourth using molecular biology improve strain. Second extremely important because they derived such as agricultural agro-industrial wastes rich in cellulose, hemicellulose, pectin, starch impregnated with lignin, hydrolyzed after delignification by physio-chemical or biological pretreatments ligninases. There lot research on their production chemical enzymatic hydrolysis, but not economically viable comparison formation several inhibitors hydrolysis high cost enzymes. Furthermore, need multiple enzymes various types carbohydrates feedstocks makes process too expensive. This article examines involved bioethanol, highly acceptable biofuel.

Язык: Английский

Процитировано

1