Abstract
Residual
antibiotics
in
chicken
manure
may
interfere
with
the
stability
of
anaerobic
digestion
(AD)
and
inhibit
resource
utilization
efficiency.
In
this
study,
we
aimed
to
enhance
bio-methanation
AD
high-concentration
sulfamethazine
(SMZ)
by
adding
metal
(Fe,
Co)-modified
Enteromorpha
-based
hydrochar
(Co-HC,
Fe-HC).
The
results
showed
that
Fe-HC
Co-HC
increased
degree
acidogenesis
1.25
times
1.58
times,
respectively.
maximum
protein
concentration
EPS
was
47.64%
72.5%
after
Co-HC.
However,
only
demonstrated
notable
improvements
both
methane
production
SMZ
removal
Electrochemical
analysis
possessed
a
richer
variety
oxygen
nitrogen
functional
groups,
along
superior
electron
exchange
capabilities
compared
Fe-HC.
Furthermore,
microbiological
assessments
revealed
enriched
syntrophic
bacteria
(such
as
Syntrophomonas
Mesotoga
),
facilitating
direct
interspecies
transfer
(DIET)
subsequently
enhancing
biomethane
production.
abundance
genes
involved
significantly
Co-HC,
increase
75.86%
Co1.5-HC
treatment.
Additionally,
elimination
antibiotic
resistance
(
sul1
,
sul2
)
65.66%
This
study
offers
theoretical
foundation
empirical
support
for
synergistic
improvement
livestock
poultry
containing
high
concentrations,
thereby
helping
overcome
challenges
posed
recalcitrant
substances.
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