Frontiers in Chemistry, Год журнала: 2024, Номер 12
Опубликована: Окт. 3, 2024
Eliciting anti-tumor immune responses and improving the tumor microenvironment crucial for boosting effectiveness of anti-PD-1 immunotherapy. Tumor-associated macrophages (TAMs), primary types cells infiltrating tumors, play a critical role in formation an immunosuppressive microenvironment. In this study, we constructed novel Evans Blue (EB)-based
Язык: Английский
Chem, Год журнала: 2024, Номер 10(7), С. 2031 - 2073
Опубликована: Июль 1, 2024
Язык: Английский
Процитировано
14
Materials Today Bio, Год журнала: 2024, Номер 28, С. 101217 - 101217
Опубликована: Авг. 24, 2024
Cold exposure therapy (CE), as an inexpensive method, has shown great potential in cancer therapy. Exploring the combined anti-tumor mechanism of CE and traditional therapies (such photodynamic (PDT)) is exciting promising. Here, a bionic aggregation-induced emission photosensitizer system (named THL) designed for to enhance immunotherapy. THL inherits homologous targeting ability tumor derived exosomes, promoting enrichment at site. Under external illumination, generates hydroxyl radicals superoxide anions through type I PDT. In addition, mice are pretreated with cold exposure, which promotes mediated PDT reactive oxygen species (ROS) generation by reducing production ATP GSH tissue. This combination increases ROS within tumor, inhibits growth distant tumors, recurrent rechallenged tumors number cytotoxic CD8+T cells memory T cells. Compared alone, shows greater advantages The strategy provides new ideas
Язык: Английский
Процитировано
10
Frontiers in Immunology, Год журнала: 2024, Номер 15
Опубликована: Апрель 29, 2024
Tumor immunotherapy is a promising approach for addressing the limitations of conventional tumor treatments, such as chemotherapy and radiotherapy, which often have side effects fail to prevent recurrence metastasis. However, effectiveness sustainability immune activation in remain challenging. immunogenic cell death, characterized by release substances, damage associated molecular patterns (DAMPs), antigens, from dying cells (DTCs), offers potential solution. By enhancing immunogenicity DTCs through inclusion more antigens stimulating factors, death (ICD) based cancer vaccines can be developed powerful tool immunotherapy. Integrating ICD nanoinducers into treatments like chemotherapy, photodynamic therapy, photothermal sonodynamic radiotherapy presents novel strategy enhance treatment efficacy potentially improve patient outcomes. Preclinical research has identified numerous inducers. effectively translating these findings clinically relevant applications remains critical challenge. This review aims contribute this endeavor providing valuable insights vitro preparation ICD-based vaccines. We explored established tools induction, followed an exploration personalized induction strategies vaccine designs. sharing knowledge, we hope stimulate further development advancement field
Язык: Английский
Процитировано
5
International Journal of Nanomedicine, Год журнала: 2025, Номер Volume 20, С. 991 - 1020
Опубликована: Янв. 1, 2025
Owing to its high prevalence, colossal potential of chemoresistance, metastasis, and relapse, breast cancer (BC) is the second leading cause cancer-related fatalities in women. Several treatments (eg, chemotherapy, surgery, radiations, hormonal therapy, etc.) are conventionally prescribed for treatment BC; however, these associated with serious systemic aftermaths. In this research, we aimed design a multiprong targeting strategy concurrent action against different phenotypes BC (MCF-7 SK-BR-3) tumor-associated macrophages (TAMs) relapse-free BC. Paclitaxel (PTX) tamoxifen (TMX) co-loaded chitosan (CS) nanoparticles (NPs) were prepared using ionic-gelation method optimized Design Expert® software by controlling material attributes. For selective through CD44-receptors that heavily expressed on cells TAMs, fabricated NPs (PTX-TMX-CS-NPs) functionalized hyaluronic acid (HA) as ligand. The HA-PTX-TMX-CS-NPs exhibited desired physicochemical properties (PS ~230 nm, PDI 0.30, zeta ~21.5 mV), smooth spherical morphology, encapsulation efficiency (PTX ~72% TMX ~97%), good colloidal stability, biphasic release kinetics. Moreover, lowest cell viability depicted MCF-7 (~25%), SK-BR-3 (~20%), RAW 264.7 induction apoptosis, cycle arrest, enhanced internalization, alleviation migration proved superior anticancer compared unfunctionalized other control medicines. HA-functionalization promising CD44-receptors-mediated TAMs mitigate progression, relapse
Язык: Английский
Процитировано
0
ACS Applied Materials & Interfaces, Год журнала: 2025, Номер unknown
Опубликована: Фев. 13, 2025
Single-atom nanozymes are highly effective in the preparation of tumor vaccines (TV) due to their superior peroxidase (POD) activity and excellent biocompatibility. However, immunosuppressive environment within tumors can diminish efficacy these vaccines. Cold exposure (CE) therapy, a noninvasive straightforward antitumor method, not only suppresses metabolism but also ameliorates milieu. In this study, we developed personalized TV using copper single-atom nanozyme (Cu SAZ) enhanced long-term by introducing CE. We initially synthesized Cu SAZ via high-temperature carbonization, which demonstrated robust POD photothermal characteristics. Upon 808 nm laser irradiation, generated reactive oxygen species (ROS) heat, inducing immunogenic cell death 4T1 breast cancer cells or CT26 colon facilitating production. our vivo prevention treatment model, noted that CE significantly boosted TV. The primary mechanism involves CE's ability lower ratio myeloid-derived suppressor (MDSCs), decrease glucose cells, increase proportions CD8+ T memory cells. Collectively, findings offer promising avenues for designing innovative systems.
Язык: Английский
Процитировано
0
Biomaterials Advances, Год журнала: 2025, Номер 172, С. 214239 - 214239
Опубликована: Фев. 22, 2025
Язык: Английский
Процитировано
0
International Journal of Nanomedicine, Год журнала: 2025, Номер Volume 20, С. 3697 - 3712
Опубликована: Март 1, 2025
Imaging early-stage brain metastases from triple-negative breast cancer (TNBC) is challenging due to the blood-brain barrier (BBB). To address this issue, we developed Den-Angio-GE11, a nanoprobe engineered traverse BBB and selectively target metastatic cells. A TNBC metastasis model was established in mice through intracardiac injection of MDA-MB-231 brain-seeking cells (MDA-MB-231-BR). Metastatic lesions were longitudinally monitored using T2-weighted magnetic resonance imaging (MRI) confirmed contrast-enhanced MRI with Gadolinium-DTPA (Gd-DTPA). The Den-Angio-GE11 synthesized on polyamidoamine (PAMAM)-G5 dendrimer platform, incorporating Angiopep-2 GE11 peptides for traversal cell targeting. Dual-modal capability achieved by conjugating Gd-DTPA NIR783 near-infrared fluorescence (NIRF) imaging. demonstrated significantly enhanced affinity EGFR compared controls, as immunofluorescence staining flow cytometry assays. Brain appeared three weeks post-injection MDA-MB-231BR maintained uncompromised function another one or two weeks, lack enhancement Gd-DTPA-enhanced MRI. Compared control nanoparticles, remarkably T1 NIRF signals after administration. Histological analysis targeting For extreme-early stage (undetectable imaging), post-Den-Angio-GE11 administration successfully indicated potential lesions. Fluorescence analyses further verified targeted sporadically parenchyma. Early can be detected T1-weighted
Язык: Английский
Процитировано
0
Small, Год журнала: 2025, Номер unknown
Опубликована: Март 23, 2025
Abstract Lymphatic metastasis of gastric cancer is a challenging issue in clinical practice. Recently, copper single‐atom nanozymes (SAZ) have gained tremendous attention due to its superior peroxidase (POD) activity that has good nonocatalytic tumor therapy (NCT) capabilities, and photothermal properties. Therefore, using high‐expressing P‐selectin platelet membrane (PM) encapsulate SAZ cisplatin proposed, forming PSC nanoparticles. Due their exquisite nanoscale size the unique structure lymphatic vessels, can highly target cells invasive primary tumors metastatic lymph nodes both express CD44. It noteworthy simultaneously perform chemotherapy generate H₂O₂ under action NADPH oxidases (NOXs) further enhance catalytic increase intracellular reactive oxygen species (ROS) production. Both vitro vivo experiments demonstrated targeting elimination capability PCS system cells. In addition, transcriptomic analysis reveals + NIR induced apoptosis MFC This marks first proposal combining drugs for dual‐targeting metastasis, providing new insights into treatment metastasis.
Язык: Английский
Процитировано
0
Journal of Nanobiotechnology, Год журнала: 2025, Номер 23(1)
Опубликована: Март 25, 2025
Cuproptosis, a novel approach utilizing copper carriers to trigger programmed cell death, exhibits promise for enhancing traditional therapies and activating robust adaptive immune responses. However, the uncontrolled release of Cu ions risks triggering cuproptosis in healthy tissues, potentially causing irreversible damage. To address this, we report on use Cu-MOF (copper metal-organic framework) protective layer regulate biodegradation copper-based nanomaterials. In situ formation Cu2O nanocubes not only stabilizes material under physiological conditions but also enhances its sonodynamic therapy (SDT) capabilities by establishing Z-Scheme heterojunction. Upon SDT activation, targeted ion at tumor site triggers cascade reactions, generating reactive oxygen species (ROS) via Fenton-like processes depleting glutathione (GSH). This ROS surge, combined with effective cuproptosis, modulates immunosuppressive microenvironment, inducing immunogenic death eliminate primary tumors inhibit metastasis. study offers new paradigm controlled integration SDT, chemodynamic (CDT), immunotherapy, achieving precise tumor-targeted treatment nanomaterial degradation.
Язык: Английский
Процитировано
0
Journal of Nanobiotechnology, Год журнала: 2025, Номер 23(1)
Опубликована: Апрель 4, 2025
Nanocatalysis coupled with photothermal therapy is a potent anti-cancer approach, yet its clinical utility limited by low concentration of tumor substrate, redox interference, and risks overheating normal tissues. Herein, we propose an innovative closed-loop nanozyme approach that leverages the synergistic effects catalytic mild (mPTT) to address aforementioned challenges. The strategy features folic acid-functionalized iron single-atom catalyst (FeNC-FA), designed exhibit exceptional multienzymatic capabilities optimal response. In system, engineered FeNC-FA capable inducing reactive oxygen species (ROS) storm depleting glutathione (GSH) in specific microenvironment (TME) initiate ferroptosis. Concurrently, accumulation ROS effectively cleaves heat shock proteins (HSPs), thereby enhancing mPTT. An intriguing aspect increased temperature within TME further facilitates conversion H2O2 O2, alleviating hypoxia providing positive feedback circuit boost therapy. Additionally, advanced photoacoustic (PA) imaging allow for self-monitoring their at sites, guiding mPTT process. Taken together, it provides PA image-guided, mutually reinforced both vitro vivo. This targeted holds great promise personalized medicine applications.
Язык: Английский
Процитировано
0