Production of polyhydroxyalkanoates by engineered Halomonas bluephagenesis using starch as a carbon source

International Journal of Biological Macromolecules, Год журнала: 2024, Номер 261, С. 129838 - 129838

Опубликована: Фев. 2, 2024

Язык: Английский

---

Exploring New Strategies for Optimizing the Production of Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) from Methane and VFAs in Synthetic Cocultures and Mixed Methanotrophic Consortia

ACS Sustainable Chemistry & Engineering, Год журнала: 2024, Номер 12(11), С. 4690 - 4699

Опубликована: Март 4, 2024

In this work, the potential of a synthetic coculture and mixed methanotrophic consortium to synthesize poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) from renewable waste-based feedstocks was assessed batchwise. Methylocystis parvuscocultivated with Rhodococcus opacus Methylocystis-enriched culture previously grown on methane were subjected nutrient starvation in medium enriched valeric acid (30% w w–1 Ctot) or VFAs mixture containing acetic, propionic, butyric, acids (15% under CH4:O2 air atmosphere. For all test series, pH adjusted 7 after adding cosubstrates, negligible substrate consumption polymer production considered end point trial. Results showed that promoted PHBV accumulation both cultures regardless Interestingly, supported only presence methane. The highest contents for consortium, equal 73.7 ± 2.5% 49.6 13% w–1, respectively, obtained mixture. This study demonstrates suitability cocultures biobased cosubstrates sustainable biodegradable PHBV.

Язык: Английский

---

Novel enhancement of interfacial interaction and properties in biodegradable polymer composites using green chemically treated spent coffee ground microfiller

International Journal of Biological Macromolecules, Год журнала: 2024, Номер 266, С. 131333 - 131333

Опубликована: Апрель 3, 2024

Язык: Английский

---

Optimized Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) Production by Moderately Haloalkaliphilic Bacterium Halomonas alkalicola Ext

International Journal of Polymer Science, Год журнала: 2024, Номер 2024, С. 1 - 17

Опубликована: Янв. 19, 2024

Polyhydroxyalkanoates (PHAs) are biodegradable and biocompatible polymers that produced by microorganisms as storage materials under limited nutrition excess carbon. These PHAs have been found to be ideal for replacing synthetic plastics use in packaging biomedical applications. In this study, an alkaliphilic moderately halophilic bacterium Halomonas alkalicola Ext was isolated from Lake Simbi Nyaima western Kenya investigated PHA production. Sudan Black B Nile Red A staining showed had distinct ability accumulation of PHAs. To optimize production, the grown submerged fermentation varying culture conditions different sources concentrations carbon nitrogen. With one-factor-at-a-time (OFTA) approach, optimal yields were obtained after 72 hours at a pH 10.0, temperature 35°C, 2.5% (w/v) NaCl. The yielded highest biomass, amounts on 2% galactose 0.1% ammonium sulfate nitrogen, respectively. record yield 0.071 g g-1 with titer $1.419\pm 0.09\text{g}/\text{L}$ achieved 3.397 g/L equivalent 41.8% content. Using response surface methodology, increased 1.5% 1.44 g/L, while content improved 1.1-fold 45.57%. Polymer analysis revealed extracted poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) ( id="M2">3−HB:3−HV=92:8 ) two copolymer subunits 3-hydroxyvaryrate (3-HB) 3-hydroxybutyrate (3-HV). attained efficient conversion into PHBV high salinity alkalinity conditions.

Язык: Английский

---

All-natural, hydrophobic, strong paper straws based on biodegradable composite coatings

Journal of Cleaner Production, Год журнала: 2024, Номер 453, С. 142243 - 142243

Опубликована: Апрель 17, 2024

Язык: Английский

---

Pilot scale-up production of poly(3-hydroxybutyrate) (PHB) from starch-based wastewater: a halogen-free process optimization for polymer recovery.

Journal of Cleaner Production, Год журнала: 2024, Номер 463, С. 142657 - 142657

Опубликована: Май 23, 2024

Язык: Английский

---

Polyhydroxyalkanoates (PHAs) and its copolymer nanocarrier application in cancer treatment: An overview and challenges

International Journal of Biological Macromolecules, Год журнала: 2024, Номер 277, С. 134201 - 134201

Опубликована: Июль 26, 2024

Язык: Английский

---

Methanotrophic and heterotrophic co-cultures for the Polyhydroxybutyrate production by co-utilizing C1 and C3 gaseous substrates

Bioresource Technology, Год журнала: 2025, Номер unknown, С. 132111 - 132111

Опубликована: Янв. 1, 2025

Язык: Английский

---

Metabolic engineering of Salinivibrio sp. TGB10 for PHBV biosynthesis with a high 3-hydroxyvalerate fraction from starch and propionate

International Journal of Biological Macromolecules, Год журнала: 2025, Номер unknown, С. 142359 - 142359

Опубликована: Март 1, 2025

Язык: Английский

---

Variation of Young's modulus suggested the main active sites for four different aging plastics at an early age time

Journal of Hazardous Materials, Год журнала: 2024, Номер 470, С. 134189 - 134189

Опубликована: Март 31, 2024

Язык: Английский

---