Endoprotein-activating DNAzyme assay for nucleic acid extraction- and amplification-free detection of viable pathogenic bacteria

Biosensors and Bioelectronics, Год журнала: 2024, Номер 266, С. 116715 - 116715

Опубликована: Авг. 29, 2024

Язык: Английский

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Integrating AI and advanced spectroscopic techniques for precision food safety and quality control

Trends in Food Science & Technology, Год журнала: 2024, Номер unknown, С. 104850 - 104850

Опубликована: Дек. 1, 2024

Язык: Английский

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Trends in the use of Argonaute Proteins in Molecular Diagnosis

TrAC Trends in Analytical Chemistry, Год журнала: 2024, Номер unknown, С. 118081 - 118081

Опубликована: Ноя. 1, 2024

Язык: Английский

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Machine Learning-Assisted, Dual-Channel CRISPR/Cas12a Biosensor-In-Microdroplet for Amplification-Free Nucleic Acid Detection for Food Authenticity Testing

ACS Nano, Год журнала: 2024, Номер 18(49), С. 33505 - 33519

Опубликована: Дек. 2, 2024

The development of novel detection technology for meat species authenticity is imperative. Here, we developed a machine learning-supported, dual-channel biosensor-in-microdroplet platform named CC-drop (CRISPR/Cas12a digital single-molecule microdroplet biosensor). This strategy allowed us to quickly identify and analyze animal-derived components in foods. biosensor was enabled by CRISPR/Cas12a-based fluorescence lighting-up detection, the nucleic acid signals can be recognized Cas12a–crRNA binary complex trigger trans-cleavage any by-stander reporter single-stranded (ss) DNA, which converted amplified fluorescent readouts. ultralocalized reactor constructed reducing reaction volume from up picoliter accommodate aforementioned further enhance sensitivity even render an amplification-free detection. Moreover, established smartphone App coupled with random forest learning model based on parameters such as area, intensity, counting number ensure accuracy image recording processing. sample-to-result time within 80 min. Importantly, proposed able accurately detect ND1 (pork-specific) IL-2 (duck-specific) genes deep processed meat-derived foods that usually had truncated results were more robust practical than conventional real-time polymerase chain after side-by-side comparison. All all, expected used rapid food other detections future.

Язык: Английский

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Cutting‐Edge Technology for Early Intervention in Myocardial Infarction: Portable Fingertips‐Based Immunobiosensor

Advanced Sensor Research, Год журнала: 2024, Номер 3(7)

Опубликована: Март 10, 2024

Abstract Early intervention in acute myocardial infarction can minimize damage and improve patient survival. Herein, a low‐cost device‐free portable immunobiosensing platform for flexible monitoring of immediate is reported. CuS‐Pt nanofragments (CuS‐Pt NFs) with high photothermal conversion efficiency (≈26.41%) are synthesized by liquid‐phase polarity‐mediated synthesis. The CuS NFs loaded situ platinum (Pt) nanoreactors using solvothermal reduction strategy, which employed to enhance the gas production. resulting nanocatalysts encapsulated within liposomes signal cascade amplification. Specifically, cardiac troponin I (cTn I), target biomarker serum, captured on pre‐modified microtiter plates formed into classical sandwich model. thermo‐chemically kinetically enhanced reactor released through one‐step chemical treatment transferred closed generator. Under excitation near‐infrared laser emitter, internal pressure generator device increases time drives carbon quantum dot solution connected hose. moving distance shows correlation concentration. This work provides new implementation development low‐cost, efficient immunosensors without requirement readout device.

Язык: Английский

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Versatile fluorescence biosensors based on CRISPR/Cas12a for determination of site-specific DNA methylation from blood and tissues

Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy, Год журнала: 2024, Номер 329, С. 125520 - 125520

Опубликована: Дек. 2, 2024

Язык: Английский

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