Micro and Nano Systems Letters,
Год журнала:
2024,
Номер
12(1)
Опубликована: Дек. 4, 2024
Abstract
Organoids
are
three-dimensional
cell
clusters
derived
from
stem
cells
and
closely
resemble
the
physiological
characteristics
of
human
tissues.
As
next-generation
biological
model,
organoids
provide
new
opportunities
for
drug
discovery,
disease
modeling,
personalized
medicine.
To
fully
harness
potential
organoids,
real-time
monitoring
states
functional
evaluation
crucial.
This
review
highlights
recent
advances
in
real-time,
situ
biosensing
technologies,
including
microelectrode
arrays
electrophysiological
recordings,
chemical
sensors
biochemical
detection,
strain
mechanical
properties.
While
development
miniature
non-invasive,
long-term,
is
early
stage,
these
an
essential
part
organoid
technology
which
would
insights
into
developmental
biology,
pathophysiology,
discovery.
After
reviewing
seminal
works
on
microfabricated
we
also
outlook
field
a
discussion
remaining
challenges
future
directions
with
focus
integration
multiple
to
facilitate
research
applications.
We
investigate
the
enhancement
in
efficiency
of
organic
bulk
heterojunction
solar
cells
enabled
by
plasmonic
excitation
Ag
nanoparticles
(NPs)
different
diameters
(10,
20,
and
30
nm),
randomly
incorporated
within
an
optical
spacing
layer
TiO2
placed
between
medium
cathode.
Such
structures
significantly
increase
absorption
photocurrent
device
system,
leading
to
a
power
conversion
more
than
4%,
over
2.5
times
that
control
cell.
This
corresponds
61%
JSC
6.3%
fill
factor.
3D
Finite-difference
time-domain
simulations
were
utilized
field
coupling
nanogap
TiO2.
They
show
nanoparticle
thin
film
cathode
extends
wavelength
range
local
beyond
obtained
for
isolated
NPs,
providing
better
overlap
with
spectrum
medium.
Molecules,
Год журнала:
2025,
Номер
30(3), С. 432 - 432
Опубликована: Янв. 21, 2025
Colored
proteins
play
an
important
role
in
synthetic
biology
research,
providing
a
systematic
labeling
tool
for
visualizing
microscopic
biological
activities
vivo.
They
can
exhibit
visible
colors
to
the
naked
eye
under
natural
light,
and
some
of
them
are
well-known
fluorescent
proteins.
Here,
several
colored
were
taken
into
consideration
acting
as
biocolorants
Escherichia
coli,
including
green
(eGFP
sfGFP),
red
protein
(mKate2),
three
chromoproteins
(GfasPurple,
AmilCP,
AeBlue).
All
significantly
change
their
bacterial
colonies.
The
color
GfasPurple
was
much
more
stable
after
heat
treatments
at
65
°C
with
75%
or
95%
ethanol.
In
addition,
factors
commonly
occurring
conditions
that
lead
dissolution,
such
heat,
ethanol,
H2O2,
vitamin
C,
acid,
alkali
treatments,
further
tested
on
GfasPurple.
Visual
observation
absorption
spectroscopy
analysis
results
showed
excellent
tolerance
against
these
unfriendly
conditions.
could
withstand
temperatures
2
h
70
1
aqueous
solutions,
but
it
fades
rapidly
50%
is
80%
ethanol
than
which
be
attributed
its
poor
solubility
high-concentration
light
curves
basically
not
affected
by
physiological
concentrations
C
reversible
effects
H2O2
found.
maintains
within
pH
range
7–11;
chromophore
will
suffer
irreversible
damage
when
up
thirteen
low
three.
These
suggest
biocolorant
far
beyond
application
prokaryotes.
Furthermore,
variants
created
via
mutagenesis
expanded
library
chromoproteins,
have
potential
value
manipulation
living
organisms.
Sensors,
Год журнала:
2025,
Номер
25(5), С. 1341 - 1341
Опубликована: Фев. 22, 2025
In
the
development,
evaluation,
and
application
of
medical
flat-panel
detectors,
modulation
transfer
function
(MTF)
is
crucial,
as
it
reflects
device’s
ability
to
restore
detailed
information.
Medical
detectors
encompass
both
image
data
acquisition
digitization
processes,
with
varying
pixel
sizes
exhibit
differing
capabilities
for
observing
details.
Accurately
quantifying
MTF
a
critical
challenge.
The
complexity
calculation,
combined
unclear
principles
details,
may
result
in
erroneous
outcomes,
thereby
misleading
research
decision-making
processes.
This
paper
presents
an
improved
oversampling
method
based
on
slit
model.
testing
conducted
under
various
sample
conditions
using
different
focal
spot
diameters
X-ray
tube
analyze
impact
size.
High-precision
tungsten
plates
fixtures
are
designed
fabricated,
results
line
spread
(LSF)
sampling
intervals
compared.
demonstrate
that
model
offers
distinct
advantages,
measurements
achieving
92.4%
ideal
value.
Compared
traditional
edge
point
(aperture)
methods,
accuracy
proposed
by
5–13%.
optimal
interval
approximately
1/29
pitch,
offering
more
accurate
evaluating
detector
performance.
Applied Sciences,
Год журнала:
2025,
Номер
15(5), С. 2634 - 2634
Опубликована: Фев. 28, 2025
The
analysis
of
gunshot
residues
(GSRs)
is
a
critical
component
criminal
investigations,
linking
suspects
to
firearms
or
shooting
incidents.
Among
the
various
analytical
techniques
employed,
scanning
electron
microscopy
(SEM)
has
emerged
as
valuable
tool
due
its
ability
provide
high-resolution
imaging
and
detailed
elemental
composition
GSR
particles.
Recent
technological
advancements
have
significantly
enhanced
effectiveness
SEM
in
analysis,
incorporating
improved
detectors
software
that
facilitate
more
accurate
detection
characterization
To
ensure
reliability
SEM-based
it
essential
adhere
established
methodologies
for
sample
collection
preparation,
well
implement
best
practices
data
interpretation
within
forensic
context.
Through
narrative
review,
this
paper
aims
explore
application
elucidate
methodological
approaches
underpin
effective
highlight
advantages
limitations
SEM,
thereby
addressing
ongoing
challenges
opportunities
field.
American Heart Journal Plus Cardiology Research and Practice,
Год журнала:
2025,
Номер
53, С. 100520 - 100520
Опубликована: Март 1, 2025
The
development
of
vascularized
organoids
as
novel
modelling
tools
the
human
cardio-cerebrovascular
system
for
preclinical
research
has
become
an
essential
platform
studying
tissues/organs
personalized
therapeutics
during
recent
decades.
Organ-on-chip
technology
is
promising
investigating
physiological
in
vitro
responses
drug
screening
and
advanced
disease
models.
Vascularized
tissue/organ-on-a-chip
benefits
every
step
discovery
pipeline
a
tool
with
close
genome
relevance
to
investigate
systems
biology.
Simultaneously,
cardio-cerebrovascular-on-chip-integrated
microfluidic
serves
alternative
animal
(patho-)physiological
processes
blood
vessels
embryonic
disease.
Integrated
next-generation
techniques,
such
three-dimensional
bioprinting
both
cells
matrix,
may
enable
organoid-on-chip-based
therapeutics.
