Root-Secreted Coumarins and the Microbiota Interact to Improve Iron Nutrition in Arabidopsis

Cell Host & Microbe, Год журнала: 2020, Номер 28(6), С. 825 - 837.e6

Опубликована: Окт. 6, 2020

Plants benefit from associations with a diverse community of root-colonizing microbes. Deciphering the mechanisms underpinning these beneficial services are interest for improving plant productivity. We report plant-beneficial interaction between Arabidopsis thaliana and root microbiota under iron deprivation that is dependent on secretion plant-derived coumarins. Disrupting this pathway alters impairs growth in iron-limiting soil. Furthermore, improves performance via mechanism import coumarin fraxetin. This trait strain specific yet functionally redundant across phylogenetic lineages microbiota. Transcriptomic elemental analyses revealed commensals coumarins promotes by relieving starvation. These results show improve eliciting microbe-assisted nutrition. propose bacterial microbiota, stimulated secreted coumarins, an integral mediator adaptation to soils.

Язык: Английский

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Disease-induced changes in plant microbiome assembly and functional adaptation

Microbiome, Год журнала: 2021, Номер 9(1)

Опубликована: Сен. 15, 2021

The plant microbiome is an integral part of the host and increasingly recognized as playing fundamental roles in growth health. Increasing evidence indicates that rhizosphere recruits beneficial microbes to suppress soil-borne pathogens. However, ecological processes govern assembly functions below- aboveground compartments under pathogen invasion are not fully understood. Here, we studied bacterial fungal communities associated with 12 (e.g., soils, roots, stems, fruits) chili pepper (Capsicum annuum L.) using amplicons (16S ITS) metagenomics approaches at main production sites China investigated how Fusarium wilt disease (FWD) affects assembly, co-occurrence patterns, plant-associated microbiomes.The amplicon data analyses revealed FWD affected less on reproductive organs (fruit) than vegetative (root stem), strongest impact upper stem epidermis. Fungal intra-kingdom networks were stable their more sensitive communities. analysis microbial interkingdom network further indicated destabilized induced importance taxa. Although diseased plants susceptible colonization by other pathogenic fungi, can also recruit potential bacteria. Some taxa enriched identified core for microbiomes hub networks. On hand, metagenomic significant enrichment several functional genes involved detoxification, biofilm formation, plant-microbiome signaling pathways (i.e., chemotaxis) plants.Together, demonstrate a could bacteria mitigate changes organ facilitate or its offspring survival. may attract through modulation pathways. These findings significantly advance our understanding interactions provide important harnessing sustainable agriculture. Video abstract.

Язык: Английский

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Disruption of Firmicutes and Actinobacteria abundance in tomato rhizosphere causes the incidence of bacterial wilt disease

The ISME Journal, Год журнала: 2020, Номер 15(1), С. 330 - 347

Опубликована: Окт. 7, 2020

Enrichment of protective microbiota in the rhizosphere facilitates disease suppression. However, how disruption rhizobacteria affects suppression is largely unknown. Here, we analyzed microbial community a healthy and diseased tomato plant grown <30-cm apart greenhouse at three different locations South Korea. The abundance Gram-positive Actinobacteria Firmicutes phyla was lower soil (DRS) than (HRS) without changes causative Ralstonia solanacearum population. Artificial bacteria HRS using 500-μg/mL vancomycin increased bacterial wilt occurrence tomato. To identify HRS-specific plant-protective species, Brevibacterium frigoritolerans HRS1, Bacillus niacini HRS2, Solibacillus silvestris HRS3, luciferensis HRS4 were selected from among 326 heat-stable culturable isolates. These four strains did not directly antagonize R. but activated immunity. A synthetic comprising these displayed greater immune activation against extended protection by 4 more days comparison with each individual strain. Overall, our results demonstrate for first time that dysbiosis DRS promotes incidence disease.

Язык: Английский

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Evidence for the plant recruitment of beneficial microbes to suppress soil‐borne pathogens

New Phytologist, Год журнала: 2020, Номер 229(5), С. 2873 - 2885

Опубликована: Ноя. 1, 2020

Summary An emerging experimental framework suggests that plants under biotic stress may actively seek help from soil microbes, but empirical evidence underlying such a ‘cry for help’ strategy is limited. We used integrated microbial community profiling, pathogen and plant transcriptive gene quantification culture‐based methods to systematically investigate three‐way interaction between the wheat plant, wheat‐associated microbiomes Fusarium pseudograminearum ( Fp ). A clear enrichment of dominant bacterium, Stenotrophomonas rhizophila (SR80), was observed in both rhizosphere root endosphere Fp‐ infected wheat. SR80 reached 3.7 × 10 7 cells g −1 accounted up 11.4% microbes endosphere. Its abundance had positive linear correlation with load at base stems expression multiple defence genes top leaves. Upon re‐introduction soils, enhanced growth, below‐ground above‐ground, induced strong disease resistance by boosting above‐ground parts, only when present. Together, bacterium seems have acted as an early warning system defence. This work provides novel potential protection against pathogens enriched beneficial microbe via modulation immune system.

Язык: Английский

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Microbiota-mediated disease resistance in plants

PLoS Pathogens, Год журнала: 2019, Номер 15(6), С. e1007740 - e1007740

Опубликована: Июнь 13, 2019

Язык: Английский

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