Animal Models and Experimental Medicine,
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 25, 2025
Abstract
Background
Rabies
virus
(RABV)–derived
neuronal
tracing
tools
are
extensively
applied
in
retrograde
due
to
their
strict
transsynaptic
transfer
property
and
low
neurotoxicity.
However,
the
RABV
infection
expression
of
fluorescence
products
would
be
gradually
cleared
while
infected
neurons
still
survive,
a
phenomenon
known
as
non‐cytolytic
immune
clearance
(NCLIC).
This
introduced
risk
loss
led
omission
subset
that
should
labeled,
thereby
interfering
analysis
results.
Methods
To
compensate
for
problem,
this
study,
we
developed
novel
marker
footprints
(MF)
mouse,
involving
Cre
recombinase‐dependent
red
fluorescent
reporter
system
systemic
glycoprotein
(G)
ASLV‐A
receptor
(TVA).
Using
mouse
model
combined
with
well‐developed
RABV‐EnvA‐ΔG‐GFP‐Cre
viral
tool,
green‐to‐red
spectral
labeling
strategy.
Results
Neurons
MF
could
co‐labeled
green
from
very
quick
tool
relatively
slow
neuron
itself,
so
undergoing
NCLIC
relabeled
red.
Furthermore,
newly
labeled
other
yellow
temporal
difference
between
two
proteins.
Conclusions
is
first
polysynaptic
strategy
label
using
colors
only
one
injection
enabling
its
application
recognizing
sequence
brain
regions
enhancing
spatiotemporal
resolution
tracing.
Scientific Reports,
Год журнала:
2023,
Номер
13(1)
Опубликована: Сен. 27, 2023
The
Neuroscience
Monoclonal
Antibody
Sequencing
Initiative
(NeuroMabSeq)
is
a
concerted
effort
to
determine
and
make
publicly
available
hybridoma-derived
sequences
of
monoclonal
antibodies
(mAbs)
valuable
neuroscience
research.
Over
30
years
research
development
efforts
including
those
at
the
UC
Davis/NIH
NeuroMab
Facility
have
resulted
in
generation
large
collection
mouse
mAbs
validated
for
To
enhance
dissemination
increase
utility
this
resource,
we
applied
high-throughput
DNA
sequencing
approach
immunoglobulin
heavy
light
chain
variable
domain
from
source
hybridoma
cells.
resultant
set
was
made
as
searchable
sequence
database
(neuromabseq.ucdavis.edu)
sharing,
analysis
use
downstream
applications.
We
enhanced
utility,
transparency,
reproducibility
existing
mAb
by
using
these
develop
recombinant
mAbs.
This
enabled
their
subsequent
engineering
into
alternate
forms
with
distinct
modes
detection
multiplexed
labeling,
miniaturized
single
fragments
or
scFvs.
NeuroMabSeq
website
corresponding
antibody
together
serve
public
repository
an
open
resource
enhancing
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Март 25, 2024
The
rise
of
large
scientific
collaborations
in
neuroscience
requires
systematic,
scalable,
and
reliable
data
management.
How
this
is
best
done
practice
remains
an
open
question.
To
address
this,
we
conducted
a
science
survey
among
currently
active
U19
grants,
funded
through
the
NIH’s
BRAIN
Initiative.
was
answered
by
both
liaisons
Principal
Investigators,
speaking
for
∼500
researchers
across
21
nation-wide
collaborations.
We
describe
tools,
technologies,
methods
use,
identify
several
shortcomings
current
practice.
Building
on
survey,
develop
plans
propose
policies
to
improve
collection,
publication,
re-use
training
community.
Frontiers in Education,
Год журнала:
2025,
Номер
10
Опубликована: Март 11, 2025
General
Education
(GE)
courses
field
students
from
different
majors
with
varied
preconceptions
of
the
life
sciences,
and
neuroscience
in
particular.
To
aid
instruction,
outcomes,
assessment
students,
core
concepts
are
an
effective
tool
that
utilizes
conceptual
elements
to
promote
learning
transfer
knowledge
between
disciplines.
This
study
examined
students'
prior
understanding
two
shared
across
biology
neuroscience—structure-function
relationship
evolution—within
student
population
enrolled
GE
courses.
The
structure-function
concept
focuses
on
how
characteristics
structures
enable
or
constrain
their
function
vice
versa,
while
evolution
similarities
differences
nervous
systems
organisms
shaped
by
ancestry
adaptations
environments.
Responses
were
analyzed
using
a
deductive
coding
approach
aimed
classify
responses
based
proficiency
either
within
general
context
neuroscience-specific
context.
Analysis
revealed
majority
non-biologists
at
start
introductory
course
unable
demonstrate
comprehension
(83.4%)
(67.0%)
Further
inductive
identified
common
themes
emerged
responses,
revealing
Structure-function
Evolution
major.
These
findings
can
educators
informing
selection
background
information
during
design
presentation
material
positively
shape
these
classes.
Animal Models and Experimental Medicine,
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 25, 2025
Abstract
Background
Rabies
virus
(RABV)–derived
neuronal
tracing
tools
are
extensively
applied
in
retrograde
due
to
their
strict
transsynaptic
transfer
property
and
low
neurotoxicity.
However,
the
RABV
infection
expression
of
fluorescence
products
would
be
gradually
cleared
while
infected
neurons
still
survive,
a
phenomenon
known
as
non‐cytolytic
immune
clearance
(NCLIC).
This
introduced
risk
loss
led
omission
subset
that
should
labeled,
thereby
interfering
analysis
results.
Methods
To
compensate
for
problem,
this
study,
we
developed
novel
marker
footprints
(MF)
mouse,
involving
Cre
recombinase‐dependent
red
fluorescent
reporter
system
systemic
glycoprotein
(G)
ASLV‐A
receptor
(TVA).
Using
mouse
model
combined
with
well‐developed
RABV‐EnvA‐ΔG‐GFP‐Cre
viral
tool,
green‐to‐red
spectral
labeling
strategy.
Results
Neurons
MF
could
co‐labeled
green
from
very
quick
tool
relatively
slow
neuron
itself,
so
undergoing
NCLIC
relabeled
red.
Furthermore,
newly
labeled
other
yellow
temporal
difference
between
two
proteins.
Conclusions
is
first
polysynaptic
strategy
label
using
colors
only
one
injection
enabling
its
application
recognizing
sequence
brain
regions
enhancing
spatiotemporal
resolution
tracing.
