Journal of Bacteriology,
Год журнала:
2024,
Номер
206(2)
Опубликована: Янв. 16, 2024
ABSTRACT
In
Cupriavidus
metallidurans
and
other
bacteria,
biosynthesis
of
the
essential
biochemical
cofactor
tetrahydrofolate
(THF)
initiates
from
guanosine
triphosphate
(GTP).
This
step
is
catalyzed
by
FolE_I-type
GTP
cyclohydrolases,
which
are
either
zinc-dependent
FolE_IA-type
or
metal-promiscuous
FolE_IB-type
enzymes.
As
THF
also
for
biosynthesis,
synthesis
form
a
cooperative
cycle,
may
be
influenced
cellular
homeostasis
zinc
metal
cations.
Metal-resistant
C.
harbors
one
two
All
three
proteins
were
produced
in
Escherichia
coli
.
FolE_IA
was
indeed
dependent
FolE_IB
enzymes
cyclohydrolases
vitro
,
latter,
example,
functioning
with
iron,
manganese,
cobalt.
Single
double
mutants
deletions
folE_I
genes
constructed
to
analyze
contribution
individual
under
various
conditions.
required
presence
cadmium,
hydrogen
peroxide,
chelators,
general
starvation
FolE_IB1
important
when
uptake
impaired
cells
without
importer
ZupT
(ZIP
family)
trimethoprim,
an
inhibitor
biosynthesis.
FolE_IB2
needed
conditions
low
cobalt
but
high
magnesium
availability.
Together,
these
data
demonstrate
that
requires
all
allow
efficient
growth
variety
IMPORTANCE
Tetrahydrofolate
microbial
biochemistry.
“Achilles
heel”
metabolism
has
been
exploited
anti-metabolites
antibiotics
such
as
sulfonamide
trimethoprim.
Since
(GTP)
starts
GTP,
both
compounds
forms
cycle.
The
first
(FolEs)
zinc-
enzymes,
so
cycle
several
cations,
especially
zinc.
metal-resistant
bacterium
needs
FolEs
grow
environments
cadmium
content.
Consequently,
bacterial
guarantee
Advanced Science,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 30, 2025
Abstract
Zinc
plays
a
central
role
in
the
hematological
development.
Therapeutic
interventions
with
zinc
are
shown
to
improve
health
status
of
patients
malignancies
by
stimulating
immune
system
and
reducing
side
effects.
Despite
abnormal
homeostasis
leukemia,
mechanisms
signaling
leukemia
development
remain
poorly
understood.
Recently,
some
important
breakthroughs
made
laboratory
clinical
studies
such
as
regulating
ferroptosis
effects
immunotherapy.
Zinc‐based
strategies
urgently
needed
refine
current
intervention
regimen
for
side‐effect
free
therapy
chemotherapy‐intolerant
patients.
This
review
provides
comprehensive
overview
focuses
on
therapeutic
potential
modulation
leukemia.
Reproductive Medicine and Biology,
Год журнала:
2025,
Номер
24(1)
Опубликована: Янв. 1, 2025
Endometriosis
is
a
prevalent
gynecological
disorder,
yet
data
on
the
role
of
trace
metal
elements
in
its
risk
remain
limited.
We
aimed
to
investigate
relationship
between
serum
copper
(Cu),
zinc
(Zn),
iron
(Fe),
magnesium
(Mg)
levels,
and
Cu/Zn
ratio
with
endometriosis.
This
study
involved
568
infertile
patients
diagnosed
endometriosis,
compared
819
without
endometriosis
(Control
group).
Basic
characteristics,
hormonal
parameters,
essential
were
measured
analyzed.
The
findings
indicated
notable
decrease
Zn
levels
group
controls,
alongside
significant
increase
(p
<
0.001).
Restricted
cubic
spline
(RCS)
analysis
revealed
linear
risk.
Moreover,
exhibited
negative
correlation
trend
=
0.005),
while
displayed
positive
risk,
even
after
adjusting
for
age,
body
mass
index
(BMI),
baseline
hormones
Compared
first
quartile
adjustment,
odds
ratios
(ORs)
95%
confidence
intervals
(CIs)
second
fourth
quartiles
1.97
(1.37,
2.83)
2.63
(1.80,
3.84),
respectively.
provided
evidence
decreased
an
increased
being
associated
elevated
among
patients.
These
offer
valuable
real-world
data,
enriching
our
understanding
Journal of Bacteriology,
Год журнала:
2024,
Номер
206(2)
Опубликована: Янв. 16, 2024
ABSTRACT
In
Cupriavidus
metallidurans
and
other
bacteria,
biosynthesis
of
the
essential
biochemical
cofactor
tetrahydrofolate
(THF)
initiates
from
guanosine
triphosphate
(GTP).
This
step
is
catalyzed
by
FolE_I-type
GTP
cyclohydrolases,
which
are
either
zinc-dependent
FolE_IA-type
or
metal-promiscuous
FolE_IB-type
enzymes.
As
THF
also
for
biosynthesis,
synthesis
form
a
cooperative
cycle,
may
be
influenced
cellular
homeostasis
zinc
metal
cations.
Metal-resistant
C.
harbors
one
two
All
three
proteins
were
produced
in
Escherichia
coli
.
FolE_IA
was
indeed
dependent
FolE_IB
enzymes
cyclohydrolases
vitro
,
latter,
example,
functioning
with
iron,
manganese,
cobalt.
Single
double
mutants
deletions
folE_I
genes
constructed
to
analyze
contribution
individual
under
various
conditions.
required
presence
cadmium,
hydrogen
peroxide,
chelators,
general
starvation
FolE_IB1
important
when
uptake
impaired
cells
without
importer
ZupT
(ZIP
family)
trimethoprim,
an
inhibitor
biosynthesis.
FolE_IB2
needed
conditions
low
cobalt
but
high
magnesium
availability.
Together,
these
data
demonstrate
that
requires
all
allow
efficient
growth
variety
IMPORTANCE
Tetrahydrofolate
microbial
biochemistry.
“Achilles
heel”
metabolism
has
been
exploited
anti-metabolites
antibiotics
such
as
sulfonamide
trimethoprim.
Since
(GTP)
starts
GTP,
both
compounds
forms
cycle.
The
first
(FolEs)
zinc-
enzymes,
so
cycle
several
cations,
especially
zinc.
metal-resistant
bacterium
needs
FolEs
grow
environments
cadmium
content.
Consequently,
bacterial
guarantee
