Chemoreceptor co-expression in Drosophila melanogaster olfactory neurons

eLife, Год журнала: 2022, Номер 11

Опубликована: Апрель 20, 2022

olfactory neurons have long been thought to express only one chemosensory receptor gene family. There are two main families in

Язык: Английский

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Differential mosquito attraction to humans is associated with skin-derived carboxylic acid levels

Cell, Год журнала: 2022, Номер 185(22), С. 4099 - 4116.e13

Опубликована: Окт. 1, 2022

Some people are more attractive to mosquitoes than others, but the mechanistic basis of this phenomenon is poorly understood. We tested mosquito attraction human skin odor and identified who exceptionally or unattractive mosquitoes. These differences were stable over several years. Chemical analysis revealed that highly produce significantly carboxylic acids in their emanations. Mutant lacking chemosensory co-receptors Ir8a, Ir25a, Ir76b severely impaired scent, retained ability differentiate weakly people. The link between elevated "mosquito-magnet" phenotypes genetic mutations acid receptors suggests such compounds contribute differential attraction. Understanding why some humans others provides insights into what odorants most important could inform development effective repellents.

Язык: Английский

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Common principles for odour coding across vertebrates and invertebrates

Nature reviews. Neuroscience, Год журнала: 2024, Номер 25(7), С. 453 - 472

Опубликована: Май 28, 2024

Язык: Английский

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Cross-modal sensory compensation increases mosquito attraction to humans

Science Advances, Год журнала: 2025, Номер 11(1)

Опубликована: Янв. 1, 2025

Sensory compensation occurs when loss of one sense leads to enhanced perception by another sense. We have identified a previously undescribed mechanism sensory in female Aedes aegypti mosquitoes. Odorant receptor co-receptor ( Orco ) mutants show attraction human skin temperature and increased heat-evoked neuronal activity foreleg neurons. Ir140 , foreleg-enriched member the ionotropic (IR) superfamily receptors, is up-regulated mutant legs. double do not heat seeking seen single mutants, suggesting that up-regulation key underlying mutants. Because expression sparse legs, this requires an indirect, long-range mechanism. Our findings highlight how mosquitoes, despite suffering olfactory loss, maintain overall effectiveness their host-seeking behavior up-regulating temperature, further enhancing status as most dangerous predator humans.

Язык: Английский

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Food hardness preference reveals multisensory contributions of fly larval gustatory organs in behaviour and physiology

PLoS Biology, Год журнала: 2025, Номер 23(1), С. e3002730 - e3002730

Опубликована: Янв. 30, 2025

Food presents a multisensory experience, with visual, taste, and olfactory cues being important in allowing an animal to determine the safety nutritional value of given substance. Texture, however, remains surprisingly unexplored aspect, despite providing key information about state food through properties such as hardness, liquidity, granularity. perception is achieved by specialised sensory neurons, which themselves are defined receptor genes they express. While it was assumed that neurons respond one or few closely related stimuli, more recent findings challenge this notion support evidence certain broadly tuned. In Drosophila taste system, gustatory opposing hedonic valence cues. Here, we identified larvae ingest navigate towards specific substrate hardnesses probed role organs behaviour. By developing genetic tool targeting specifically organs, show these major contributors for evaluation hardness ingestion decision-making. We find ablation not only results loss chemosensation, but also navigation preference varied hardnesses. Furthermore, primary organ exhibit concurrent physiological responses mechanical multimodal stimulation. individual house independent mechanisms multiple modalities, challenging assumptions capabilities neurons. propose further investigations, across kingdom, may reveal higher complexity than currently anticipated.

Язык: Английский

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Biology and Behaviour of Aedes aegypti in the Human Environment: Opportunities for Vector Control of Arbovirus Transmission

Viruses, Год журнала: 2023, Номер 15(3), С. 636 - 636

Опубликована: Фев. 27, 2023

is a ubiquitous vector of arboviruses mostly in urbanised areas throughout the tropics and subtropics growing threat beyond. Control

Язык: Английский

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Olfactory navigation in arthropods

Journal of Comparative Physiology A, Год журнала: 2023, Номер 209(4), С. 467 - 488

Опубликована: Янв. 20, 2023

Abstract Using odors to find food and mates is one of the most ancient highly conserved behaviors. Arthropods from flies moths crabs use broadly similar strategies navigate toward odor sources—such as integrating flow information with information, comparing concentration across sensors, over time. Because arthropods share many homologous brain structures—antennal lobes for processing olfactory mechanosensors flow, mushroom bodies (or hemi-ellipsoid bodies) associative learning, central complexes navigation, it likely that these closely related behaviors are mediated by neural circuits. However, differences in types they seek, physics dispersal, locomotion water, air, on substrates mean circuits must have adapted generate a wide diversity odor-seeking In this review, we discuss common specializations observed navigation behavior arthropods, review our current knowledge about subserving behavior. We propose comparative study arthropod nervous systems may provide insight into how set basic circuit structures has diversified different environments.

Язык: Английский

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Sparse and stereotyped encoding implicates a core glomerulus for ant alarm behavior

Cell, Год журнала: 2023, Номер 186(14), С. 3079 - 3094.e17

Опубликована: Июнь 15, 2023

Ants communicate via large arrays of pheromones and possess expanded, highly complex olfactory systems, with antennal lobes in the brain comprising up to ∼500 glomeruli. This expansion implies that odors could activate hundreds glomeruli, which would pose challenges for higher-order processing. To study this problem, we generated transgenic ants expressing genetically encoded calcium indicator GCaMP sensory neurons. Using two-photon imaging, mapped complete glomerular responses four ant alarm pheromones. Alarm robustly activated ≤6 activity maps three inducing panic our species converged on a single glomerulus. These results demonstrate that, rather than using broadly tuned combinatorial encoding, employ precise, narrowly tuned, stereotyped representations The identification central hub glomerulus behavior suggests simple neural architecture is sufficient translate pheromone perception into behavioral outputs.

Язык: Английский

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Sox21b underlies the rapid diversification of a novel male genital structure between Drosophila species

Current Biology, Год журнала: 2024, Номер 34(5), С. 1114 - 1121.e7

Опубликована: Фев. 2, 2024

The emergence and diversification of morphological novelties is a major feature animal evolution.1York J.R. McCauley D.W. origin evolution vertebrate neural crest cells.Open Biol. 2020; 10190285Crossref PubMed Scopus (32) Google Scholar,2Tomoyasu Y. What crustaceans can tell us about the insect wings other morphologically novel structures.Curr. Opin. Genet. Dev. 2021; 69: 48-55Crossref (7) Scholar,3Moczek A.P. On origins novelty diversity in development evolution: case study on beetle horns.Cold Spring Harbor Symp. Quant. 2009; 74: 289-296Crossref (0) Scholar,4Kijimoto T. Pespeni M. Beckers O. Moczek Beetle horns horned beetles: emerging models developmental ecology.Wiley Interdiscip. Rev. 2013; 2: 405-418Crossref (33) Scholar,5Rebeiz Tsiantis Enhancer novelty.Curr. 2017; 45: 115-123Crossref (63) Scholar,6Shubin N. Tabin C. Carroll S. Deep homology evolutionary novelty.Nature. 457: 818-823Crossref (585) Scholar,7Bruce H.S. Patel N.H. Daphnia carapace structures evolved via cryptic persistence serial homologs.Curr. 2022; 32: 3792-3799.e3Abstract Full Text PDF Scholar,8Colizzi E.S. Hogeweg P. Vroomans R.M.A. Modelling novelty: review.Essays Biochem. 66: 727-735Crossref (2) Scholar,9Muller G.B. Wagner G.P. Novelty Evolution: Restructuring Concept.Annu. Ecol. Syst. 1991; 22: 229-256Crossref Scholar However, relatively little known genetic basis mechanisms underlying their diversification. epandrial posterior lobes male genitalia are particular Drosophila species.10Glassford W.J. Johnson W.C. Dall N.R. Smith S.J. Liu Boll W. Noll Rebeiz Co-option an Ancestral Hox-Regulated Network Underlies Recently Evolved Morphological Novelty.Dev. Cell. 2015; 34: 520-531Abstract (75) Scholar,11Jagadeeshan Singh R.S. A time-sequence functional analysis mating behavior genital coupling Drosophila: role female choice sex-drive genitalia.J. Evol. 2006; 19: 1058-1070Crossref Scholar,12Yassin A. Orgogozo V. Coevolution between Male Female Genitalia melanogaster Species Subgroup.PLoS One. 8e57158Crossref (72) Scholar,13Kopp True Evolution sexual characters Oriental species group.Evol. 2002; 4: 278-291Crossref (111) grasp ovipositor insert her abdominal tergites and, therefore, important for copulation recognition.10Glassford Scholar,14Frazee S.R. Harper A.R. Afkhami Wood M.L. McCrory J.C. Masly J.P. Interspecific introgression reveals morphology during reproductive isolation Drosophila.Evolution. 75: 989-1002Crossref (3) Scholar,15Frazee Multiple selection pressures drive rapid complex secondary structure.Ecol. 5: 4437-4450Crossref (29) Scholar,16LeVasseur-Viens H. Polak Moehring A.J. No evidence external affecting 1797-1807Crossref (20) Scholar,17Robertson H.M. Mating asymmetries phylogeny complex.Pac. Sci. 1988; 42: 72-80Google likely from co-option Hox-regulated gene network spiracles10Glassford have since diversified D. simulans clade, particular, over last 240,000 years, driven by selection.18McDermott Kliman R.M. Estimation Isolation Times Island Complex Multilocus DNA Sequence Data.PLOS 2008; 3e2442Crossref (47) Scholar,19Coyne J.A. Genetic Basis Differences Genital Morphology Among Three Sibling Drosophila.Evolution (NY). 1983; 37: 1101-1118Crossref Scholar,20House C.M. Lewis Z. Hodgson D.J. Wedell Sharma M.D. Hunt J. Hosken Sexual Natural Selection Both Influence Evolution.PLoS 8e63807Crossref (43) Scholar,21House simulans.Evolution. 501-514Crossref (1) this polygenic but, to best our knowledge, none causative genes been identified.22Liu Mercer J.M. Stam L.F. Gibson G.C. Zeng Z.-B. Laurie C.C. Analysis Shape Difference mauritiana.Genetics. 1996; 142: 1129-1145Crossref Scholar,23Zeng Z.B. Kao C.H. architecture shape difference two species.Genetics. 2000; 154: 299-310Crossref Scholar,24LeVasseur-Viens Individual Contributions Variation mauritiana.Int. 2014; 2014808247Crossref Scholar,25True Quantitative Divergence Secondary Traits Between mauritiana.Evolution. 1997; 51: 816-832Crossref Scholar,26Tanaka K.M. Hopfen Herbert M.R. Schlötterer Stern D.L. McGregor Nunes M.D.S. Architecture Functional Characterization Genes Underlying Rapid Diversification External 200: 357-369Crossref (26) Scholar,27Hagen J.F.D. Mendes Booth Figueras Jimenez Tanaka Franke F.A. Baudouin-Gonzalez L. Ridgway A.M. Arif et al.Unraveling Species.Mol. 38: 437-448Crossref Scholar,28Hackett J.L. Wang X. B.R. Macdonald Mapping QTL Contributing Posterior Lobe Strains melanogaster.PLoS 2016; 11e0162573Crossref Scholar,29Laurie An Introgression Trait Loci That Contribute 145: 339-348Crossref Scholar,30Masly Dalton J.E. Srivastava Chen Arbeitman M.N. Rapidly Evolving Drosophila.Genetics. 2011; 189: 357-374Crossref (35) Identifying these essential understanding impact recognition. Here, we show that Sox21b negatively regulates lobe size. This consistent with expanded expression mauritiana, which develops smaller than simulans. We tested generating reciprocal hemizygotes confirmed changes underlie species. Furthermore, found size differences caused species-specific allele significantly affect duration. Taken together, sexual-selection-driven structure its copulatory behavior.

Язык: Английский

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Mosquito Cell Atlas: A single-nucleus transcriptomic atlas of the adultAedes aegyptimosquito

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Фев. 25, 2025

The female mosquito's remarkable ability to hunt humans and transmit pathogens relies on her unique biology. Here, we present the Mosquito Cell Atlas (MCA), a comprehensive single-nucleus RNA sequencing dataset of more than 367,000 nuclei from 19 dissected tissues adult male Aedes aegypti , providing cellular-level resolution mosquito We identify novel cell types expand our understanding sensory neuron organization chemoreceptors all tissues. Our analysis uncovers male-specific cells sexually dimorphic gene expression in antenna brain. In mosquitoes, find that glial brain, rather neurons, undergo most extensive transcriptional changes following blood feeding. findings provide insights into cellular basis behavior sexual dimorphism. MCA aims serve as resource for vector biology community, enabling systematic investigation cell-type specific across

Язык: Английский

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