bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2023,
Номер
unknown
Опубликована: Май 10, 2023
ABSTRACT
Cells
live
and
interact
in
three-dimensional
(3D)
cellular
neighborhoods.
However,
histology
spatial
omics
methods
mostly
focus
on
2D
tissue
sections.
Here
we
present
a
3D
atlas
of
routine
clinical
sample,
an
aggressive
human
lung
carcinoma,
by
combining
situ
quantification
960
cancer-related
genes
across
∼340,000
cells
with
measurements
tissue-mechanical
components.
neighborhoods
subdivided
the
tumor
microenvironment
into
tumor,
stromal,
immune
multicellular
niches.
Interestingly,
pseudotime
analysis
suggested
that
pro-invasive
epithelial-to-mesenchymal
transition
(EMT),
detected
stroma-infiltrating
cells,
already
occurred
one
region
at
surface.
There,
myofibroblasts
macrophages
specifically
co-localized
pre-invasive
their
molecular
signature
identified
patients
shorter
survival.
Moreover,
cytotoxic
T-cells
did
not
infiltrate
this
niche
but
colocalized
inhibitory
dendritic
regulatory
T
cells.
Importantly,
systematic
scoring
cell-cell
interactions
highlighted
niche-specific
signaling
networks
accompanying
invasion
escape.
Compared
to
2D,
improved
characterization
niches
identifying
niches,
capturing
extension
T-cell
boosting
interactions,
including
druggable
checkpoints.
We
believe
communication
analyses
can
improve
design
studies
investigating
personalized,
combination
immuno-oncology
therapies.
Frontiers in Endocrinology,
Год журнала:
2025,
Номер
15
Опубликована: Янв. 8, 2025
This
study
aimed
to
investigate
the
alterations
in
diabetes
risk
associated
with
sarcopenia
and
insufficient
physical
activity,
as
well
demographic
shifts
within
diabetic
population.
Utilizing
pertinent
data
from
National
Health
Nutrition
Examination
Survey
(NHANES)
database
spanning
2011
2018,
criteria
for
were
established
by
Foundation
Institutes
of
Health.
These
calculated
using
lean
body
mass
conjunction
index
data.
Physical
activity
levels
assessed
PAQ
questionnaire
NHANES
database.
The
presence
was
determined
through
DIQ
laboratory
examination
analysis
performed
multivariable
logistic
regression.
prevalence
both
population
188%
greater
than
non-diabetic
Sarcopenia
positively
correlated
an
increased
onset,
demonstrating
a
1.45-fold
heightened
when
conditions
present
(OR=2.45,95%CI,1.35-4.44,P<0.05).
combined
effect
significantly
alone
(OR=1.84,95%CI,1.09-3.11,P<0.05)
or
(OR=1.55,95%CI,1.11-2.15,P<0.05).
A
synergistic
relationship
exists
between
resulting
markedly
elevated
are
concurrently.
Therefore,
comprehensive
management
strategies
should
prioritize
populations
exhibiting
activity.
iScience,
Год журнала:
2025,
Номер
28(3), С. 111934 - 111934
Опубликована: Фев. 4, 2025
Three-dimensional
(3D)
optical
microscopy
permits
in
situ
interrogation
of
the
tumor
microenvironment
(TME)
volumetric
tumors
for
research
while
light
sheet
and
confocal
fluorescence
are
often
used
to
achieve
macroscopic
microscopic
3D
images
tissues,
respectively.
Although
each
technique
offers
distinct
fields
view
(FOVs)
spatial
resolution,
combination
two
obtain
correlative
multiscale
from
same
tissues
has
not
yet
been
explored.
We
established
a
workflow
that
enables
tracking
imaging
region
interests
(ROIs)
within
through
sequential
microscopy.
This
approach
allowed
quantitative
analysis
immune
response
TME
at
multiple
scales
facilitated
direct
localization
metastatic
lesion
mouse
brain.
Our
method
an
with
potential
provide
new
insights
into
comprehensive
disease
mechanism
or
drug
response.
Cardiovascular
diseases
constitute
a
marked
threat
to
global
health,
and
the
emergence
of
spatial
omics
technologies
has
revolutionized
cardiovascular
research.
This
review
explores
application
omics,
including
transcriptomics,
proteomics,
metabolomics,
genomics,
epigenomics,
providing
more
insight
into
molecular
cellular
foundations
disease
highlighting
critical
contributions
science,
discusses
future
prospects,
technological
advancements,
integration
multi-omics,
clinical
applications.
These
developments
should
contribute
understanding
guide
progress
precision
medicine,
targeted
therapies,
personalized
treatments.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2023,
Номер
unknown
Опубликована: Май 10, 2023
ABSTRACT
Cells
live
and
interact
in
three-dimensional
(3D)
cellular
neighborhoods.
However,
histology
spatial
omics
methods
mostly
focus
on
2D
tissue
sections.
Here
we
present
a
3D
atlas
of
routine
clinical
sample,
an
aggressive
human
lung
carcinoma,
by
combining
situ
quantification
960
cancer-related
genes
across
∼340,000
cells
with
measurements
tissue-mechanical
components.
neighborhoods
subdivided
the
tumor
microenvironment
into
tumor,
stromal,
immune
multicellular
niches.
Interestingly,
pseudotime
analysis
suggested
that
pro-invasive
epithelial-to-mesenchymal
transition
(EMT),
detected
stroma-infiltrating
cells,
already
occurred
one
region
at
surface.
There,
myofibroblasts
macrophages
specifically
co-localized
pre-invasive
their
molecular
signature
identified
patients
shorter
survival.
Moreover,
cytotoxic
T-cells
did
not
infiltrate
this
niche
but
colocalized
inhibitory
dendritic
regulatory
T
cells.
Importantly,
systematic
scoring
cell-cell
interactions
highlighted
niche-specific
signaling
networks
accompanying
invasion
escape.
Compared
to
2D,
improved
characterization
niches
identifying
niches,
capturing
extension
T-cell
boosting
interactions,
including
druggable
checkpoints.
We
believe
communication
analyses
can
improve
design
studies
investigating
personalized,
combination
immuno-oncology
therapies.
