bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2025,
Номер
unknown
Опубликована: Март 2, 2025
SUMMARY
Receptor-like
kinases
(RLKs),
particularly
the
Transmembrane
Kinase
(TMK)
family,
play
essential
roles
in
signaling
and
development,
with
TMKs
being
key
components
of
auxin
perception
downstream
phosphorylation
events.
While
TMKs’
involvement
canalization,
a
process
for
vasculature
formation
regeneration,
has
been
established,
nonetheless,
additional
regulatory
partners
remain
poorly
understood.
In
this
study,
we
identify
characterize
seven
leucine-rich
repeat
RLKs
(TINT1–TINT7)
as
novel
interactors
TMK1,
revealing
their
diverse
evolutionary,
structural,
functional
characteristics.
Our
results
show
that
TINTs
interact
TMK1
highlight
regulating
various
developmental
processes.
Majority
contributes,
together
to
TINT5
linking
other
canalization
component
CAMEL.
Beyond
also
establish
role
TINT-TMK1
interactions
processes
such
stomatal
movement
hypocotyl’s
gravitropic
response.
These
findings
suggest
TINTs,
through
interaction
are
integral
networks,
contributing
both
broader
plant
development.
Journal of Experimental Botany,
Год журнала:
2024,
Номер
75(18), С. 5471 - 5476
Опубликована: Май 23, 2024
Abstract
The
TIR1/AFB–Aux/IAA–ARF
canonical
auxin
signaling
pathway
is
widely
accepted
to
(de)active
transcriptional
regulation,
thus
controlling
auxin-associated
developmental
processes.
However,
the
theme
of
a
rapid
response
has
emerged
since
2018
Auxins
and
Cytokinin
in
Plant
Development
conference.
To
date,
few
components
have
been
identified
mediate
both
slow
responses,
which
unveils
complexity
signaling.
iScience,
Год журнала:
2024,
Номер
27(7), С. 110363 - 110363
Опубликована: Июнь 24, 2024
Indole-3-propionic
acid
(IPA)
is
known
to
be
a
microbe-derived
compound
with
similar
structure
the
phytohormone
auxin
(indole-3-acetic
acid,
IAA).
Previous
studies
reported
that
IPA
exhibited
auxin-like
bioactivities
in
plants.
However,
underlying
molecular
mechanism
not
totally
understood.
Here,
we
revealed
modulated
lateral
root
(LR)
development
via
signaling
model
plant
Proceedings of the National Academy of Sciences,
Год журнала:
2024,
Номер
121(51)
Опубликована: Дек. 10, 2024
The
classical
acid
growth
theory
suggests
that
auxin
stimulates
cell
expansion
by
triggering
apoplast
acidification
via
plasma
membrane
(PM)-localized
H
+
-ATPase.
Here,
we
reconstructed
the
origin
and
evolutionary
history
of
auxin-mediated
growth.
Comparative
phylogenomic
analysis
showed
most
core
components
originated
in
Charophyta
then
underwent
subclass
functional
innovation
during
plant
terrestrialization.
In
Charophyceae
algae
Chara
braunii,
found
PM
-ATPase
has
formed
a
regulatory
module
with
TMK
PP2C.D,
which
can
be
activated
photosynthesis-dependent
phosphorylation
through
light
rather
than
auxin.
Despite
lack
canonical
receptor
TRANSPORT
INHIBITOR
RESPONSE
1/AUXIN
SIGNALING
F-BOX
(TIR1/AFB),
elicits
significant
internodal
elongation
transcriptional
reprogramming
C.
braunii
,
implying
existence
an
ancient
mechanism.
We
propose
evolution
represents
neofunctional
adaptation
to
terrestrial
environments,
carbon
concentrating
for
photosynthesis
was
utilized
acidify
expansion,
responsible
eventually
established
network
land
plants
connecting
TIR1/AFB
pathway.
Abstract
Plants,
as
sessile
organisms,
must
adapt
to
a
range
of
abiotic
stresses,
including
drought,
salinity,
heat,
and
cold,
which
are
increasingly
exacerbated
by
climate
change.
These
stresses
significantly
impact
crop
productivity,
posing
challenges
for
sustainable
agriculture
food
security.
Recent
advances
in
omics
studies
genetics
have
shed
light
on
molecular
mechanisms
underlying
plant
stress
responses,
the
role
calcium
(Ca
2
⁺)
signaling,
liquid–liquid
phase
separation
(LLPS),
cell
wall-associated
sensors
detecting
responding
environmental
changes.
However,
gaps
remain
understanding
how
rapid
signaling
is
integrated
with
slower,
adaptive
processes.
Emerging
evidence
also
highlights
crosstalk
between
immunity,
growth
regulation,
mediated
key
components
such
RAF-SnRK2
kinase
cascades,
DELLA
proteins,
etc.
Strategies
enhance
resistance
without
compromising
yield
include
introducing
beneficial
alleles,
spatiotemporal
optimization
decoupling
from
inhibition.
This
review
emphasizes
importance
interdisciplinary
approaches
innovative
technologies
bridge
fundamental
research
practical
agricultural
applications,
aiming
develop
resilient
crops
production
an
era
escalating
challenges.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2025,
Номер
unknown
Опубликована: Март 2, 2025
SUMMARY
Receptor-like
kinases
(RLKs),
particularly
the
Transmembrane
Kinase
(TMK)
family,
play
essential
roles
in
signaling
and
development,
with
TMKs
being
key
components
of
auxin
perception
downstream
phosphorylation
events.
While
TMKs’
involvement
canalization,
a
process
for
vasculature
formation
regeneration,
has
been
established,
nonetheless,
additional
regulatory
partners
remain
poorly
understood.
In
this
study,
we
identify
characterize
seven
leucine-rich
repeat
RLKs
(TINT1–TINT7)
as
novel
interactors
TMK1,
revealing
their
diverse
evolutionary,
structural,
functional
characteristics.
Our
results
show
that
TINTs
interact
TMK1
highlight
regulating
various
developmental
processes.
Majority
contributes,
together
to
TINT5
linking
other
canalization
component
CAMEL.
Beyond
also
establish
role
TINT-TMK1
interactions
processes
such
stomatal
movement
hypocotyl’s
gravitropic
response.
These
findings
suggest
TINTs,
through
interaction
are
integral
networks,
contributing
both
broader
plant
development.
