From single species to communities: microsatellite amplicon sequencing to monitor felids using Feliplex DOI Creative Commons
Divyashree Rana, Frédéric Boyer, Marta De Barba

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Окт. 23, 2024

Abstract The current biodiversity crisis demands a shift from single-species to multispecies approaches in conservation, particularly for rare and endangered species. However, this transition requires tools optimised research, which are currently limited. Recent advances high-throughput sequencing (HTS) technologies bioinformatics have enabled efficient robust acquisition of genetic data. Amplicon approaches, particular, demonstrated potential enhancing non-invasive studies species, but their application has been mostly limited single To enable we introduce cost-effective HTS-based amplicon approach genotyping multiple species simultaneously, designed population monitoring, including individual identification ascertaining patterns structure. We developed Feliplex, multiplex panel 85 co-amplifying tetranucleotide microsatellite markers cross-genotyping Felidae demonstrate the utility our approach. Feliplex was validated on known samples nine Indian felid across genera Panthera, Prionailurus , Felis . applied it invasive (blood tissue) (hair faeces) DNA extracts 173 wild individuals obtaining respectively 70% 56% multilocus success rates. accurately identified clusters tigers ( Panthera tigris n=19) revealed hitherto unknown structure fishing cats viverrinus n=40). Feliplex’s wide applicability allows reliable low-quality/quantity samples, while supporting conservation monitoring lesser-known like small cats. Our broad can be adapted develop similar panels closely related groups.

Язык: Английский

Empowering Regional Conservation: Genetic Diversity Assessments as a Tool for Eelgrass Management DOI Creative Commons
E. C. Faust, Kristie Rigby, Anders Olsson

и другие.

Molecular Ecology, Год журнала: 2025, Номер unknown

Опубликована: Янв. 12, 2025

To halt the loss of biodiversity, collaboration among scientists, managers and decision-makers is vital. Although biodiversity a global problem, management actions influencing diversity are often on local to regional scale. Our study an example conservation genomic assessment developed in between scientists managers. We used 2bRAD sequencing assess 18 eelgrass (Zostera marina) meadows northwestern Sweden, area that has experienced large losses since 1980s. Genetic was comparable other assessed Atlantic, but order magnitude lower than Pacific. All one meadow showed high rates sexual reproduction. Almost all were divergent grouped into five genetic clusters. Four clusters correspond geographic regions can be define units. Meadows areas with decline 1980s 2020s more inbred increase eelgrass. Overall, our results indicate striving protect number within each cluster important for maintaining connectivity Sweden likely beneficial wider ecosystem. estimate current indicators essential variables discuss their challenges marine facultative clonal species. showcase how regional-scale assessments serve as foundation protection restoration priority

Язык: Английский

Процитировано

0
Demographic history of the Malayan tapirs (Tapirus indicus) in Southeast Asia DOI Creative Commons
Qi Luan Lim, Yu Sato, Norsyamimi Rosli

и другие.

Ecological Research, Год журнала: 2025, Номер unknown

Опубликована: Янв. 27, 2025

Язык: Английский

Процитировано

0
Conservation Genetics of the Endangered Danube Clouded Yellow Butterfly Colias myrmidone (Esper, 1780) in the Last Central European Stronghold: Diversity, Wolbachia Infection and Balkan Connections DOI Creative Commons
Aleksandra Gwiazdowska, Robert Rutkowski, Marcin Sielezniew

и другие.

Insects, Год журнала: 2025, Номер 16(2), С. 220 - 220

Опубликована: Фев. 17, 2025

The Danube Clouded Yellow (Colias myrmidone) has experienced one of the most dramatic declines among European butterflies. To estimate genetic diversity in last population Poland that survived Knyszyn Forest (KF), we analyzed mitochondrial (COI) and nuclear (EF-1α) polymorphisms individuals sampled 2014 2022. results were compared with data obtained from a recently extirpated nearby (Czerwony Bór, CB). Because mtDNA insects can be modulated by endosymbionts, samples screened for Wolbachia. polymorphism EF-1α indicated was gradually decreasing. KF rapid demographic processes, manifested significant change allele frequency. small differentiation markers between CB suggests regional used to genetically uniform. Four COI haplotypes identified this study probably belong two different haplogroups. Wolbachia detected only specific haplotype, prevalence female-biased, suggesting induction reproductive manipulations. common haplotype found same as reported other parts Europe, not C. myrmidone but also caucasica. These allow us question distinctiveness each taxa.

Язык: Английский

Процитировано

0
Editorial: Challenges and prospects for conservation genetics at XXI century DOI Creative Commons
Patricia Susana Amavet, Gabriela Paula Fernández, Viviana G. Solís Neffa

и другие.

Frontiers in Genetics, Год журнала: 2025, Номер 16

Опубликована: Фев. 18, 2025

A molecular tool that has been developed in recent years is metabarcoding, which enables the identification of species' genetic material from complex samples, such as environmental samples. Mannise et al. utilized metabarcoding to analyze diet Neotropical foxes by extracting DNA feces. This approach exemplifies one non-invasive sampling techniques increasingly favored studies avoid unnecessary harm living specimens. The authors' findings were consistent with previous based on morphological consumed items, demonstrating effective traditional species methods. Nonetheless, there are challenges address, particularly regarding accuracy analysis methods, occurrence false positives or negatives (Corse al., 2019). These issues can be prevented using appropriate universal primers, rigorous negative and PCR controls, careful results supported bibliographic references.On other hand, markers, nuclear markers (e.g., microsatellites) mitochondrial continue widely used for identification, demonstrated study Gonzalez authors propose presence marsh deer (Blastocerus dichotomus) Uruguay past, extending geographic range. have also proven researching diversity structure puma (Puma concolor) populations central southern Argentina (Mac Allister al.) allowing definition two distinct Management Units (MU) south distribution. combined use different allowed Oklander explore evolutionary history brown howler monkey (Alouatta guariba), existence a single species, three MU. data presented all these crucial support management institutions developing more conservation strategies species.In line within field quantitative genetics, Bruno present candidate genes associated coat color Podolica Italiana Grey Cattle breed. integrates medium-density SNP array genotype bioinformatic analyses cohort multi-cohort FST-outlier approach. work growing relevance large-scale studies, driven technological advances sequencing methods over past decade. enable generation vast genomic datasets, not only require training new generations bioinformatics researchers but allow diverse applications results. In this case, direct implications animal breeding strategies. Moreover, analytical contributes deeper understanding architecture, potential beyond genetics fields human medicine.Finally, an interesting contribution Napolitano highlights key research Latin America. Based surveys individuals involved conservation, identify main barriers lack funding limited access laboratories. However, previously mentioned, advancements technologies significantly reduced costs, while development international laboratory networks fosters greater collaboration resource sharing. factors provide promising outlook overcoming near future, facilitating broader tools promoting efforts across region. small selection articles role advancing efforts. Genetic contribute identifying threats biodiversity, distribution, detecting hybridization events, defining units, exemplified van der Valk Dalèn (2024), among others. Collecting essential American countries, where information remains limited. Expanding knowledge regions optimize sustainable valuable resources, ultimately supporting biodiversity preservation at both regional global scales.

Язык: Английский

Процитировано

0
Population structure of Dugong dugon across the Indo-Pacific revealed by historical mitogenomes DOI Creative Commons
Lydia Hildebrand Furness, Oliver Kersten, Aurélie Boilard

и другие.

Royal Society Open Science, Год журнала: 2024, Номер 11(8)

Опубликована: Авг. 1, 2024

Sirenia , an iconic marine taxon with a tropical and subtropical worldwide distribution, face uncertain future. All species are designated ‘Vulnerable’ to extinction by the IUCN. Nonetheless, comprehensive understanding of geographic structuring across global range is lacking, impeding our ability highlight particularly vulnerable populations for conservation priority. Here, we use ancient DNA investigate dugong ( Dugong dugon ) population structure, analysing 56 mitogenomes from specimens comprising known historical range. Our results reveal geographically structured distinct monophyletic clades characterized contrasting evolutionary histories. We observe deep-rooted divergent lineages in East (Indo-Pacific) obtain new evidence relatively recent dispersal dugongs into western Indian Ocean. significantly differentiated each other having approximately 10-fold lower levels genetic variation than eastern Indo-Pacific populations. Additionally, find significant temporal loss diversity Ocean since mid-twentieth century, as well decline size beginning 1000 years ago. add growing body that becoming ever more susceptible ongoing human action climate change.

Язык: Английский

Процитировано

1
From single species to communities: microsatellite amplicon sequencing to monitor felids using Feliplex DOI Creative Commons
Divyashree Rana, Frédéric Boyer, Marta De Barba

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Окт. 23, 2024

Abstract The current biodiversity crisis demands a shift from single-species to multispecies approaches in conservation, particularly for rare and endangered species. However, this transition requires tools optimised research, which are currently limited. Recent advances high-throughput sequencing (HTS) technologies bioinformatics have enabled efficient robust acquisition of genetic data. Amplicon approaches, particular, demonstrated potential enhancing non-invasive studies species, but their application has been mostly limited single To enable we introduce cost-effective HTS-based amplicon approach genotyping multiple species simultaneously, designed population monitoring, including individual identification ascertaining patterns structure. We developed Feliplex, multiplex panel 85 co-amplifying tetranucleotide microsatellite markers cross-genotyping Felidae demonstrate the utility our approach. Feliplex was validated on known samples nine Indian felid across genera Panthera, Prionailurus , Felis . applied it invasive (blood tissue) (hair faeces) DNA extracts 173 wild individuals obtaining respectively 70% 56% multilocus success rates. accurately identified clusters tigers ( Panthera tigris n=19) revealed hitherto unknown structure fishing cats viverrinus n=40). Feliplex’s wide applicability allows reliable low-quality/quantity samples, while supporting conservation monitoring lesser-known like small cats. Our broad can be adapted develop similar panels closely related groups.

Язык: Английский

Процитировано

0