Comprehensive analysis of the interaction microbiome and prostate cancer: an initial exploration from multi-cohort metagenome and GWAS studies

Journal of Translational Medicine, Год журнала: 2025, Номер 23(1)

Опубликована: Янв. 29, 2025

Prostate cancer is one of the most common cancers in United States with a high mortality rate. In recent years, traditional opinion about prostate microbiome was challenged. Although there still are some arguments, an escalating number researchers shifting their focus toward within tumor environment. We mined data extracted from metagenome, and it offers broader taxonomic coverage accurate functional profiling. used Kraken2, mapping tool, to mine gut microbiota patients. A two-sample Mendelian Randomization conducted reflect association between cancer. study, we found consistency special intratumor both non-metastatic tumors metastatic tumors. And dig patients different treatments. that microbiotas may be associated progression exist. The anti-androgen therapy can significantly change microbiome. With metastasis cancer, changes. influences Our discovery help further understand progression, metastasis, resistance perspective level.

Язык: Английский

---

Exploring the prognostic role of microbial and genetic markers in lung squamous cell carcinoma

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Фев. 6, 2025

Abstract Despite advances in diagnostic and therapeutic strategies, the prognosis of lung squamous cell carcinoma (LUSC) patients remains poor, potential microbiome-based prognostic biomarkers targets largely unexplored. LUSC patient data from The Cancer Genome Atlas (TCGA), including microbial genus level abundance RNA sequencing (RNA-Seq) data, were used as a training dataset. Two other independent datasets GSE19188 GSE157009 serve validation datasets. A risk score (RS) model was constructed by univariate Cox regression analysis combined with least absolute contraction selection operator (LASSO) regression. 18 genera found to be significantly associated RFS patients. signature built these genera, exhibited robust predictive accuracy both Furthermore, hub mRNA between high- low-risk groups selected XGBOOST intersect mRNAs screened analysis, finally identifying four prognosis. This study reveals complex interplay microbiome genetic biomarkers, identifies specific microbial-based LUSC. These findings provide basis for future studies aimed elucidate mechanisms underlying associations guiding treatment decisions improving outcomes.

Язык: Английский

---

Herbal Formula Yi‐Fei‐Jie‐Du‐Tang Regulates Epithelial‐Mesenchymal Transition and Vasculogenic Mimicry in Lung Cancer via HIF1A‐Mediated Ferroptosis

Advanced Biology, Год журнала: 2025, Номер unknown

Опубликована: Фев. 6, 2025

Traditional Chinese medicine (TCM) Yi-Fei-Jie-Du-Tang (YFJDT) has shown potential in lung cancer treatment. However, the mechanisms underlying effects of YFJDT on remain unclear. Bioinformatics analysis is conducted to identify targets YFJDT. The impact hypoxia-inducible factor 1 alpha (HIF1A), ferroptosis, and vasculogenic mimicry (VM) investigated using xenograft tumor models A549 cells. Additionally, cells are stimulated with CoCl2 mimic hypoxic microenvironment tumor. role HIF1A overexpression modulating ferroptosis assessed. epithelial-mesenchymal transition (EMT) VM vitro evaluated. Results: treatment led a concentration-dependent decrease levels tumors Overexpression counteractes inhibitory proliferation, EMT, transplanted tumors. Moreover, attenuates YFJDT-induced lipid peroxidation iron accumulation, indicating inhibition Hypoxia-induced alterations EMT markers reversed by but exacerbated overexpression. Molecular docking identified salicylic acid psoralen as components targeting HIF1A. exerts anti-tumor downregulating promoting ferroptosis.

Язык: Английский

---

Intratumoral microbiome: implications for immune modulation and innovative therapeutic strategies in cancer

Journal of Biomedical Science, Год журнала: 2025, Номер 32(1)

Опубликована: Фев. 19, 2025

Abstract Recent advancements have revealed the presence of a microbiome within tumor tissues, underscoring crucial role in ecosystem. This review delves into characteristics intratumoral microbiome, its dual modulating immune responses and potential to both suppress promote growth. We examine state-of-the-art techniques for detecting analyzing bacteria, with particular focus on their interactions system resulting implications cancer prognosis treatment. By elucidating intricate crosstalk between host system, we aim uncover novel therapeutic strategies that enhance efficacy treatments. Additionally, this addresses existing challenges future prospects burgeoning field, advocating integration research comprehensive therapy frameworks. Graphical

Язык: Английский

---

Using mathematical modelling and AI to improve delivery and efficacy of therapies in cancer

Nature reviews. Cancer, Год журнала: 2025, Номер unknown

Опубликована: Фев. 19, 2025

Язык: Английский

---

Cross-laboratory replication of pseudomyxoma peritonei tumor microbiome reveals reproducible microbial signatures

mSphere, Год журнала: 2025, Номер unknown

Опубликована: Фев. 20, 2025

ABSTRACT Recent work has demonstrated that cancer-specific microbial communities often colonize tumor tissues. However, untangling low-biomass signals from environmental contamination makes this research technically challenging. We utilize pseudomyxoma peritonei (PMP), a cancer characterized by the spread of mucus-secreting cells throughout peritoneal cavity, to develop robust workflow for identifying reproducible microbiomes. Typically originating rupture an appendiceal into metastasized tumors have been previously shown harbor core set microbes. did not control potential these low biomass samples. expand upon prior findings characterizing microbiome 70 additional PMP and six normal tissues along with appropriate laboratory controls. Additionally, DNA subset 25 was extracted sequenced at independent laboratory. found evidence signatures between replicates different include taxa may be introduced surgical contamination, as well patient-specific are also commonly implicated in colorectal cancer. In addition, preoperative chemotherapy treatment reduce diversity. Our demonstrate how sample replication can powerful approach investigate associated will improve research. IMPORTANCE over 30 types The origin their possible involvement carcinogenesis or outcomes remains unclear, yet important area A current major challenge low-biomass, tumor-associated microbiomes is introduction during collection, handling, extraction, PCR, sequencing. Here, we provide framework replicating samples help identify background contamination. Using approach, show (PMP) host communities, including organisms Incorporating future studies promising increase reproducibility field.

Язык: Английский

---

De-coding the complex role of microbial metabolites in cancer

Cell Reports, Год журнала: 2025, Номер 44(3), С. 115358 - 115358

Опубликована: Март 1, 2025

SummaryThe human microbiome, an intricate ecosystem of trillions microbes residing across various body sites, significantly influences cancer, a leading cause morbidity and mortality worldwide. Recent studies have illuminated the microbiome's pivotal role in cancer development, either through direct cellular interactions or by secreting bioactive compounds such as metabolites. Microbial metabolites contribute to initiation mechanisms DNA damage, epithelial barrier dysfunction, chronic inflammation. Furthermore, microbial exert dual roles on progression response therapy modulating metabolism, gene expression, signaling pathways. Understanding these complex is vital for devising new therapeutic strategies. This review highlights promising targets prevention treatment, emphasizing their impact responses underscoring need further research into metastasis resistance.

Язык: Английский

---

Parvimonas micra promotes oral squamous cell carcinoma metastasis through TmpC-CKAP4 axis

Nature Communications, Год журнала: 2025, Номер 16(1)

Опубликована: Март 7, 2025

Parvimonas micra (P. micra), an opportunistic oral pathogen associated with multiple cancers, has limited research on its role in squamous cell carcinoma (OSCC). This study shows that P. is enriched OSCC tissues and positively correlated tumor metastasis stages. infection promotes by inducing hypoxia/HIF-1α, glycolysis, autophagy. Mechanistically, surface protein TmpC binds to CKAP4, a receptor overexpressed OSCC, facilitating bacterial attachment invasion. interaction activates HIF-1α autophagy via CKAP4-RanBP2 CKAP4-NBR1 pathways, driving metastasis. Targeting CKAP4 masitinib or antibodies impairs abolishes micra-promoted vitro vivo. Together, our findings identify as highlight TmpC-CKAP4 could be potential therapeutic target for OSCC. micra) frequently present lesions infections. Here, authors report regulation HIF-1α-induced glycolysis following binding receptor.

Язык: Английский

---

Microbial dysbiosis with tryptophan metabolites alteration in lower respiratory tract is associated with clinical responses to anti-PD-1 immunotherapy in advanced non-small cell lung cancer

Cancer Immunology Immunotherapy, Год журнала: 2025, Номер 74(4)

Опубликована: Март 8, 2025

Lower respiratory tract microbiome constitutes a unique immune microenvironment for advanced non-small cell lung cancer as one of dominant localized microbial components. However, there exists little knowledge on the associations between this regional and clinical responses to anti-PD-1 immunotherapy from perspectives. Here, we equivalently collected bronchoalveolar lavage fluids 56 NSCLC participants treated with none (untreated, n = 28) or (treated, 28), which was further divided into responder (n 17) non-responder 11) subgroups according responses, aiming compare their discrepancy by performing metagenomic sequencing targeted metabolic alterations tryptophan sequencing. Correspondingly, diversities transformed significantly after receiving immunotherapeutic agents, where Gammaproteobacteria Campylobacter enriched, but Escherichia, Streptococcus, Chlamydia, Staphylococcus reduced at genus level, differences failed be achieved among various (responder non-responder; LDA > 2, P < 0.05*). And relative abundance Streptomyces escalated in response subgroup compositional analysis (as ≥ 3%, 0.05*), no significance untreated groups. In addition, abundances bacterial metabolites its derivatives were also higher subgroup, distinctively being associated divergent genera (VIP 1, Our study revealed predictive performance lower antitumoral suggested that may alter composition interact regulate therapeutic efficacy NSCLC, potential biomarkers prognosis interventional strategies.

Язык: Английский

---

Dynamic Changes of Dental Plaque and Saliva Microbiota in OSCC Progression

Research Square (Research Square), Год журнала: 2025, Номер unknown

Опубликована: Март 11, 2025

Objectives To elucidate the microbial and genomic changes in saliva dental plaque during Oral Squamous Cell Carcinoma (OSCC) progression, to identify potential mechanisms virulence factors involved OSCC pathogenesis. Materials Methods Using metagenomic sequencing, 64 samples from patients at different stages of differentiation were examined. Results The results showed notable differences composition profiles across ecological regions degrees. Notably, relative abundance specific microbes, such as Porphyromonas gingivalis, Fusobacterium nucleatum, Haemophilus parainfluenzae, increased poorly differentiated OSCC. Microbial alpha diversity correlates with tumor T staging. Dental microbiota shows higher specialization, especially tumors. Both types become more stable advanced Genomic analysis reveals stages. Subsequently, functional pathway tracing pathogens reveal oral cancer may promote tumorigenesis by secreting like GAPDH, GspG, AllS, drive initiation progression through interactions. Conclusions is associated altered composition, diversity, plaque. Poorly show factors, implicating them tumorigenesis. Clinical Relevance Understanding could help develop new diagnostic biomarkers therapies targeting microbiota, potentially improving early detection, treatment efficacy, prognosis for patients. Maintaining microbiome homeostasis also prevent cancer.

Язык: Английский

---