Piezoelectric Polyvinylidene Fluoride-Trifluoroethylene/Reduced Graphene Oxide/Polycaprolactone Fiber Material: Modulating Neutrophil Extracellular Traps and Reshaping the Immune Microenvironment in Peripheral Nerves
Advanced Fiber Materials,
Год журнала:
2025,
Номер
unknown
Опубликована: Март 10, 2025
Язык: Английский
EP2 Modulates Satellite Glial Cell Activation in Temporomandibular Joint Osteoarthritis Chronic Pain via p-ERK1/2 Signaling
Journal of Dental Research,
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 20, 2025
The
etiology
of
chronic
pain
in
temporomandibular
joint
osteoarthritis
(TMJOA)
is
still
unclear,
making
its
treatment
challenging
clinical
practice.
Emerging
evidence
suggests
that
the
activation
satellite
glial
cells
(SGCs)
exerts
an
important
role
development
pain.
This
study
aims
to
investigate
whether
and
which
prostaglandin
E
receptor
(EP)
subtypes
expressed
on
peripheral
SGCs
how
corresponding
EP
modulate
SGC
during
TMJOA
Immunofluorescence
double
staining
was
applied
demonstrate
EP2
EP3
activated
trigeminal
ganglions
mice.
In
vitro
studies
cultivation
primary
showed
antagonist
PF-04418948
significantly
attenuated
a
dose-dependent
manner,
while
agonist
sulprostone
failed
affect
activation.
Kyoto
Encyclopedia
Genes
Genomes
analysis
RNA
sequencing
Western
blot
demonstrated
EP2-mediated
signaling
pathways
were
associated
with
phosphorylated
extracellular
signal–regulated
kinase
1
2
(p-ERK1/2)
mitogen-activated
protein
kinases
(MAPKs).
addition,
verify
involvement
vivo,
recombinant
adeno-associated
virus
vector
containing
fibrillary
acidic
protein–shRNA
(EP2)–enhanced
green
fluorescent
injected
into
mouse
ganglion
third
branch
nerve
knockdown
SGCs.
Taken
together,
modulates
through
MAPK/p-ERK1/2
monosodium
iodoacetate–induced
TMJOA.
reveals
new
mechanism
activation,
providing
insights
for
Язык: Английский
Protocol to isolate dorsal root ganglion neurons from embryonic rats by immunopanning and characterize them using RNAscope and immunofluorescence
STAR Protocols,
Год журнала:
2025,
Номер
6(2), С. 103836 - 103836
Опубликована: Май 21, 2025
Язык: Английский
Peripheral myelin: From development to maintenance
Journal of Neurochemistry,
Год журнала:
2024,
Номер
169(1)
Опубликована: Дек. 10, 2024
Abstract
Peripheral
myelin
is
synthesized
by
glial
cells
called
Schwann
(SCs).
SC
development
and
differentiation
must
be
tightly
regulated
to
avoid
any
pathological
consequence
affecting
peripheral
nerve
function.
Neuropathic
symptoms
can
arise
from
developmental
issues
in
SCs,
as
well
adult
life
through
processes
mature
SCs.
In
this
review
we
focus
on
the
immature
towards
myelinating
non‐myelinating
stages,
defining
molecular
mechanisms
outlining
radial
sorting,
a
multi‐stepped
event
essential
for
myelination.
We
also
describe
regulating
sheath
maintenance
homeostasis
during
aging.
Finally,
will
conclude
with
some
remaining
questions
field
of
biology.
image
Язык: Английский