Current Biology, Год журнала: 2024, Номер unknown
Опубликована: Дек. 1, 2024
Язык: Английский
bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown
Опубликована: Март 17, 2024
Abstract Animals are often bombarded with visual information and must prioritize specific features based on their current needs. The neuronal circuits that detect relay have been well-studied. Yet, much less is known about how an animal adjusts its attention as goals or environmental conditions change. During social behaviors, flies need to focus nearby flies. Here, we study the flow of altered when female Drosophila enter aggressive state. From connectome, identified three state-dependent circuit motifs poised selectively amplify response fly-sized objects: convergence excitatory inputs from neurons conveying select internal state; dendritic disinhibition feature detectors; a switch toggles between two detectors. Using cell-type-specific genetic tools, together behavioral neurophysiological analyses, show each these function during aggression. We reveal this same operate in males courtship pursuit, suggesting disparate behaviors may share mechanisms. Our work provides compelling example using connectome infer mechanisms underlie dynamic processing sensory signals.
Язык: Английский
Процитировано
14
bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown
Опубликована: Июнь 6, 2024
In most complex nervous systems there is a clear anatomical separation between the nerve cord, which contains of final motor outputs necessary for behaviour, and brain. insects, neck connective both physical information bottleneck connecting brain ventral cord (VNC, spinal analogue) comprises diverse populations descending (DN), ascending (AN) sensory neurons, are crucial sensorimotor signalling control. Integrating three separate EM datasets, we now provide complete connectomic description neurons female system
Язык: Английский
Процитировано
12
bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown
Опубликована: Сен. 18, 2023
Abstract Discovering principles underlying the control of animal behavior requires a tight dialogue between experiments and neuromechanical models. Until now, such models, including NeuroMechFly for adult fly, Drosophila melanogaster , have primarily been used to investigate motor control. Far less studied with realistic body models is how brain systems work together perform hierarchical sensorimotor Here we present v2, framework that expands modeling by enabling visual olfactory sensing, ascending feedback, complex terrains can be navigated using leg adhesion. We illustrate its capabilities first constructing biologically inspired locomotor controllers use feedback path integration head stabilization. Then, add sensing this controller train it reinforcement learning multimodal navigation task in closed loop. Finally, more biorealistic two ways: our model navigates odor plume taxis strategy, uses connectome-constrained system network follow another simulated fly. With framework, accelerate discovery explanatory nervous develop machine learning-based autonomous artificial agents robots.
Язык: Английский
Процитировано
11
bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown
Опубликована: Окт. 23, 2023
Controlling arms and legs requires feedback from proprioceptive sensory neurons that detect joint position movement. Proprioceptive must be tuned for different behavioral contexts, but the underlying circuit mechanisms remain poorly understood. Using calcium imaging in behaving
Язык: Английский
Процитировано
11
bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown
Опубликована: Апрель 16, 2024
In order to forage for food, many animals regulate not only specific limb movements but the statistics of locomotor behavior over time, example switching between long-range dispersal behaviors and more localized search depending on availability resources. How pre-motor circuits such is clear. Here we took advantage robust changes in evoked by attractive odors walking
Язык: Английский
Процитировано
4
Current Biology, Год журнала: 2025, Номер unknown
Опубликована: Янв. 1, 2025
Highlights•The descending neuron DNp03 in Drosophila responds to aversive looming stimuli•Its spiking activity is state dependent and correlated with flight saccades•DNp03 provides input other neurons involved controlling turns•Optogenetic activation of can initiate free-flight saccadesSummaryApproaching threats are perceived through visual looming, a rapid expansion an image on the retina. Visual triggers defensive responses such as freezing, flight, turning, or take-off wide variety organisms, from mice fish insects.1,2,3,4 In response flies perform evasive turns known saccades.5 Saccades also be initiated spontaneously change direction during flight.6,7,8,9 Two types (DNs), DNaX DNb01, were previously shown exhibit both spontaneous looming-elicited saccades Drosophila.10,11 As they do not receive direct system, it has remained unclear how visually elicited controlled by nervous system. receives looming-sensitive projection output wing motor neurons12,13 therefore promising candidate for saccades. Using whole-cell patch-clamp recordings head-fixed flying Drosophila, we showed that ipsilateral behavioral-state-dependent manner. We further explored relates variable behavioral output. Sustained activity, persisting after stimulus, was strongest predictor saccade execution. However, alone cannot fully explain variability responses. Combined optogenetic experiments free these results suggest important but exclusive role saccades, advancing our understanding information transformed into commands maneuvers insects.Graphical abstract
Язык: Английский
Процитировано
0
Scientific Reports, Год журнала: 2025, Номер 15(1)
Опубликована: Фев. 12, 2025
Abstract Our sense of taste is critical for regulating food consumption. The fruit fly Drosophila represents a highly tractable model to investigate mechanisms processing, but circuits beyond sensory neurons are largely unidentified. Here, we use whole-brain connectome the organization circuits. We trace pathways from four populations that detect different modalities and project subesophageal zone (SEZ), primary region brain. find second-order primarily located within SEZ segregated by modality, whereas third-order have more projections outside overlap between modalities. Taste out innervate regions implicated in feeding, olfactory learning. analyze interconnections pathways, characterize modality-dependent differences neuron properties, identify other types inputs onto computational simulations relate neuronal connectivity predicted activity. These studies provide insight into architecture
Язык: Английский
Процитировано
0
Cellular and Molecular Life Sciences, Год журнала: 2025, Номер 82(1)
Опубликована: Апрель 10, 2025
Abstract Rare disorders often represent a molecular deviation from hi-fidelity genomic integrity networks and are perceived as too difficult or unimportant for further mechanistic studies. Here, we synthesize evidence demonstrating how valuable knowledge of biochemical pathways related to rare can be biomedicine. To this end, describe several congenital lipid, protein, organic acid, glycan metabolism discuss phenotypes (such “ extreme responders ”) case reports the lenalidomide cases) have provided clues drug discovery repurposing. We also such Gaucher disease ultra-rare genetic syndromes provide insights into cancer mTOR-driven metabolism, respectively. Our discussion highlights continued value studies in understanding human pathophysiology even genomics era.
Язык: Английский
Процитировано
0
Proceedings of the National Academy of Sciences, Год журнала: 2025, Номер 122(16)
Опубликована: Апрель 17, 2025
In order to forage for food, many animals regulate not only specific limb movements but the statistics of locomotor behavior, switching between long-range dispersal and local search depending on resource availability. How premotor circuits is clear. Here, we analyze model their modulation by attractive food odor in walking Drosophila . Food evokes three motor regimes flies: baseline walking, upwind running during odor, behavior following loss. During search, find that flies adopt higher angular velocities slower ground speeds turn longer periods same direction. We further different mean speed these state changes influence length odor-evoked runs. next developed a simple neural control suggests contralateral inhibition plays key role regulating statistical features locomotion. As fly connectome predicts decussating inhibitory neurons lateral accessory lobe (LAL), gained genetic access subset tested effects behavior. identified one population whose activation induces all signature regulates velocity at offset. second population, including single LAL neuron pair, bidirectionally speed. Together, our work develops biologically plausible computational architecture captures locomotion across behavioral states identifies substrates computations.
Язык: Английский
Процитировано
0
Current Opinion in Neurobiology, Год журнала: 2023, Номер 84, С. 102822 - 102822
Опубликована: Дек. 13, 2023
The descending neurons connecting the fly's brain to its ventral nerve cord respond sensory stimuli and evoke motor programs of varying complexity. Anatomical characterization their synaptic connections suggests how these circuits organize movements, while optogenetic manipulation activity reveals what behaviors they can induce. Monitoring responses or during behavior performance indicates information may encode. Recent advances in all three approaches make an excellent place better understand sensorimotor integration transformation required for nervous systems govern sequences that constitute animal behavior.
Язык: Английский
Процитировано
10