Crosstalk between RNA m6A modification and epigenetic factors in plant gene regulation

Plant Communications, Год журнала: 2024, Номер 5(10), С. 101037 - 101037

Опубликована: Июль 6, 2024

N6-methyladenosine (m6A) is the most abundant modification observed in eukaryotic mRNAs. Advances transcriptome-wide m6A mapping and sequencing technologies have enabled identification of several conserved motifs plants, including RRACH (R = A/G H A/C/U) UGUAW (W U or A) motifs. However, mechanisms underlying deposition marks at specific positions individual transcripts remain to be clarified. Evidence from plant animal studies suggests that writer eraser components are recruited genomic loci through interactions with particular transcription factors, 5-methylcytosine DNA methylation marks, histone marks. In addition, recent cells shown microRNAs play a role depositing sites base-pairing mechanism. also affects biogenesis function chromatin-associated regulatory RNAs long noncoding RNAs. Although we less an understanding link between epigenetic factors plants than animals, progress identifying proteins interact has provided insights into crosstalk which plays crucial transcript-specific regulation plants.

Язык: Английский

---

High glucose facilitates hepatocellular carcinoma cell proliferation and invasion via WTAP-mediated HK2 mRNA stability

Molecular and Cellular Biochemistry, Год журнала: 2025, Номер unknown

Опубликована: Март 3, 2025

Язык: Английский

---

Interactive Structural Analysis of KH3-4 Didomains of IGF2BPs with Preferred RNA Motif Having m6A Through Dynamics Simulation Studies

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(20), С. 11118 - 11118

Опубликована: Окт. 16, 2024

m

Язык: Английский

---

N6-methyladenosine-modified long non-coding RNA KIF9-AS1 promotes stemness and sorafenib resistance in hepatocellular carcinoma by upregulating SHOX2 expression

World Journal of Gastroenterology, Год журнала: 2024, Номер 30(48), С. 5174 - 5190

Опубликована: Ноя. 29, 2024

BACKGROUND Hepatocellular carcinoma (HCC) is a prevalent and aggressive tumor. Sorafenib the first-line treatment for patients with advanced HCC, but resistance to sorafenib has become significant challenge in this therapy. Cancer stem cells play crucial role HCC. Our previous study revealed that long non-coding RNA (lncRNA) KIF9-AS1 an oncogenic gene However, of drug cancer stemness HCC remains unclear. Herein, we aimed investigate function mechanism lncRNA AIM To describe elucidate underlying mechanism. METHODS Tumor tissue adjacent non-cancerous samples were collected from patients. Sphere formation was quantified via tumor sphere assay. Cell viability, proliferation, apoptosis evaluated Counting Kit-8, flow cytometry, colony assays, respectively. The interactions between its downstream targets confirmed immunoprecipitation coimmunoprecipitation. tumorigenic validated mouse model. RESULTS Compared normal controls, expression upregulated tissues. Knockdown inhibited attenuated cells. Mechanistically, N6-methyladenosine modification mediated by methyltransferase-like 3/insulin-like growth factor 2 mRNA-binding protein 1 stabilized increased . Additionally, stability short stature homeobox promoting ubiquitin-specific peptidase 1-induced deubiquitination. Furthermore, depletion alleviated xenograft model CONCLUSION N6-methyladenosine-modified promoted upregulating expression.

Язык: Английский

---

Sperm RNA code in spermatogenesis and male infertility

Reproductive BioMedicine Online, Год журнала: 2024, Номер 49(6), С. 104375 - 104375

Опубликована: Июль 10, 2024

Язык: Английский

---

The crosstalk between cellular survival pressures and N6-methyladenosine modification in hepatocellular carcinoma

Hepatobiliary & pancreatic diseases international, Год журнала: 2024, Номер unknown

Опубликована: Авг. 1, 2024

Язык: Английский

---