Structural Features and Synteny Analysis of the Sea Buckthorn Mitochondrial Genome
Research Square (Research Square),
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 23, 2025
Abstract
Background
Sea
buckthorn
(
Hippophae
rhamnoides
L.)
is
a
plant
of
significant
economic,
culinary,
medicinal,
ecological,
and
societal
value.
In
order
to
gain
deeper
understanding
the
sea
mitochondrial
genome,
sequencing
assembly
genome
was
performed,
its
substructures
were
explored
in
this
study.
Results
The
seabuckthorn
consists
two
circular
chromosomes,
with
lengths
297,507
bp
167,037
bp,
respectively.
It
encompasses
36
annotated
protein-coding
genes,
3
rRNA
25
tRNA
genes.
addition,
212
pairs
repeats
detected,
including
pair
that
mediated
homologous
recombination
chromosomes
form
conformations.
existence
these
conformations
confirmed
through
PCR
amplification
Sanger
sequencing.
A
total
162
SSR
loci
identified
genome.
There
are
30
fragments
chloroplast
genomes,
length
44,950
accounting
for
9.89%
genomes.
Conclusions
summary,
study
conducted
annotation
providing
comprehensive
plant.
This
knowledge
great
significance
effective
utilization
genetic
improvement
seabuckthorn,
especially
breeding
evolutionary
analysis
cytoplasmic
male
sterility.
Язык: Английский
Ethnogenetic analysis reveals the Bronze Age genetic affiliation of Yashkuns with West Eurasians
Scientific Reports,
Год журнала:
2024,
Номер
14(1)
Опубликована: Дек. 28, 2024
Focusing
on
the
Yashkun
population
of
Gilgit-Baltistan,
an
administrative
territory
in
northern
Pakistan,
our
study
investigated
mtDNA
haplotypes
as
indicators
ancient
gene
flow
and
genetic
diversity.
Genomic
DNA
was
extracted
evaluated
for
quality
using
agarose
gel
electrophoresis.
The
complete
control
region
(nt
16024–576)
amplified
via
PCR,
sequencing
performed
Big
Dye
Terminator
Kit
Applied
Biosystems
Genetic
Analyzer.
Sequence
alignment
analysis
were
conducted
Geneious
software
Cambridge
Reference
(CRS).
Haplotypes
classified
into
phylogenetic
haplogroups
with
HaploGrep
2
Mito
Tool
platform,
referencing
PhyloTree
version
17.
identified
considerable
diversity
within
population,
major
macro-haplogroups
H
(29.47%),
T
(13.68%),
M
(13.68%)
representing
West
Eurasian,
South
Asian,
East
&
Southeast
Asian
lineages.
highest
affiliation
Eurasian
(73.68%),
indicating
significant
from
West.
population's
distinctiveness
highlighted
by
calculation
forensic
characteristics,
such
power
discrimination
(PD
=
0.9975),
(GD
0.9865),
random
match
probability
(RMP
0.01349).
knowledge
makeup
further
improved
inference
links
made
possible
comparisons
other
indigenous
groups
Pakistan.
These
results
attest
to
reliability
data
applications
ancestry
tracking,
individual
identification,
additions
national
databases.
inclusion
science
is
strongly
supported
this
groundbreaking
community,
especially
areas
like
Pakistan
diverse
histories.
Язык: Английский
Mutational disparities in colorectal cancers of White Americans, Alabama African Americans, And Oklahoma American Indians
npj Precision Oncology,
Год журнала:
2024,
Номер
8(1)
Опубликована: Дек. 23, 2024
Abstract
The
high
incidence
and
mortality
rates
of
colorectal
cancer
(CRC)
in
Alabama
African
Americans
(AAs)
Oklahoma
American
Indians
(AIs)
are
recognized
as
disparities,
yet
the
underlying
causes
have
been
poorly
demonstrated.
By
evaluating
CRC
whole-exome
sequencing
mutational
profiles,
here
we
report
sets
mutated
genes
whose
frequencies
differed
significantly
(
p
<
0.05)
a
race-specific
manner.
Secondary
screening
with
database
identified
“survival-critical
(SCGs)”
(i.e.,
mutations/alterations
associated
significant
differences
patients’
survival
rates)
among
differentially
genes.
Notable
SCGs
race-pronounced
variants
were
different
from
DEGs
their
involved
pathways
included
nucleotide
catabolism
cell
cycle
checkpoints
for
AAs,
extracellular
matrix
organization
AIs.
inclusion
these
clinical
next-generation
panels
development
targeting
drugs
will
serve
refinements
precision
medicine
to
overcome
racial
disparities
health
outcomes
CRC.
Язык: Английский