Aging Research, Год журнала: 2024, Номер 2(4), С. 9340034 - 9340034
Опубликована: Дек. 1, 2024
Язык: Английский
International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(1), С. 427 - 427
Опубликована: Янв. 6, 2025
Glutaminase controls the first step in glutaminolysis, impacting bioenergetics, biosynthesis and oxidative stress. Two isoenzymes exist humans, GLS GLS2. is considered prooncogenic overexpressed many tumours, while GLS2 may act as or a tumour suppressor. Glioblastoma cells usually lack they express high GLS. We investigated how expression modifies metabolism of glioblastoma cells, looking for changes that explain GLS2’s potential suppressive role. developed LN-229 stably expressing performed isotope tracing using U-13C-glutamine metabolomic quantification to analyze metabolic changes. Treatment with inhibitor CB-839 was also included concomitantly inhibit endogenous overexpression resulted extensive changes, altering TCA cycle by upregulating part but blocking synthesis 6-carbon intermediates from acetyl-CoA. Expression caused downregulation PDH activity through phosphorylation S293 PDHA1. altered nucleotide levels induced accumulation methylated metabolites S-adenosyl methionine. These suggest be key regulator linking glutamine glucose metabolism, nucleotides epigenetics. Future research should ascertain mechanisms involved generalizability these findings cancer physiological conditions.
Язык: Английский
Процитировано
1
Advanced Science, Год журнала: 2025, Номер unknown
Опубликована: Янв. 24, 2025
Abstract Recipients often suffer from hyperlactatemia during liver transplantation (LT), but whether exacerbates hepatic ischemia‐reperfusion injury (IRI) after donor implantation remains unclear. Here, the role of in IRI is explored. In this work, found to exacerbate ferroptosis IRI. Lactate‐primed lysine acetyltransferase 8 (KAT8) determined directly lactylate mitochondrial phosphoenolpyruvate carboxykinase 2 (PCK2) at Lys100 and augments PCK2 kinase activity. By using gene‐edited mice, evidence indicating that generated. Mechanistically, acts as a critical inducer by competitively inhibiting Parkin‐mediated polyubiquitination 3‐oxoacyl‐ACP synthase (OXSM), thereby leading metabolic remodeling fatty acid synthesis (mtFAS) potentiation oxidative phosphorylation tricarboxylic cycle. More importantly, targeting demonstrated markedly ameliorate hyperlactatemia‐mediated Collectively, findings support use therapeutics suppress patients with LT.
Язык: Английский
Процитировано
1
Cell Death Discovery, Год журнала: 2025, Номер 11(1)
Опубликована: Фев. 8, 2025
Abstract Histone lactylation plays a crucial role in cancer progression, but its impact on breast (BC) tumorigenesis is still unclear. We utilized chromatin immunoprecipitation sequencing with H3K18la antibodies, transcriptomics of clinical BC samples, and proteomics ATAC-seq analyses vivo tumors to identify the genes regulated by transcription factor PPARD. qPCR Western blot assays were used detect expressions molecules. discovered that levels higher tissues compared adjacent non-cancerous tissues. promoted expression PPARD, which turn influenced AKT, not ILK. analysis revealed glycolysis cells enhanced accessibility. Additionally, we confirmed HDAC2 HDAC3 act as “erasers” for H3 lysine lactylation. During analysis, AKT-phosphorylation aerobic respiration inhibitor group exhibited an apparent disparity activity. Our study demonstrated changes downstream PPARD support cell survival under anaerobic conditions. accelerated proliferation promoting phosphorylation AKT. This highlights therapeutic potential targeting H3K18la/PPARD/AKT axis cancer, providing new insights into epigenetic regulation metabolism (Trial registration: The was approved Research Ethics Committee Shandong Provincial Third Hospital (KYLL-2023057; https://www.medicalresearch.org.cn/ )).
Язык: Английский
Процитировано
1
Frontiers in Immunology, Год журнала: 2024, Номер 15
Опубликована: Июль 8, 2024
The non-natriuretic-dependent glutamate/cystine inverse transporter-system Xc- is composed of two protein subunits, SLC7A11 and SLC3A2, with serving as the primary functional component responsible for cystine uptake glutathione biosynthesis. implicated in tumor development through its regulation redox homeostasis, amino acid metabolism, modulation immune function, induction programmed cell death, among other processes relevant to tumorigenesis. In this paper, we summarize structure biological functions SLC7A11, discuss potential role therapy, which provides a new direction precision personalized treatment tumors.
Язык: Английский
Процитировано
6
Cytokine, Год журнала: 2025, Номер 187, С. 156857 - 156857
Опубликована: Янв. 11, 2025
Язык: Английский
Процитировано
0
ACS Applied Materials & Interfaces, Год журнала: 2025, Номер unknown
Опубликована: Март 4, 2025
Mitochondria, as essential cellular organelles, play a key role in numerous diseases, from neurodegenerative disorders to cancer and rare conditions. The extraction of mitochondria cells has many applications disease diagnosis, pathological research, emerging mitochondrial transplantation therapy (MTT). Recent advancements microfluidic-on-chip systems offer promising improvements by enabling high-throughput processing, precise control, flexibility while facilitating integration with other devices platforms. Despite growing interest microfluidic (MME), there is lack comprehensive reviews on the latest developments this field. This review aims summarize recent well advantages limitations MME, providing deeper insights into microfluidic-based approaches for extraction, purification, analysis.
Язык: Английский
Процитировано
0
Pharmaceuticals, Год журнала: 2024, Номер 17(7), С. 871 - 871
Опубликована: Июль 2, 2024
Hepatocellular carcinoma (HCC), currently ranking as the third most lethal malignancy, poses a grave threat to human health. Ferroptosis, form of programmed cell demise, has emerged promising therapeutic target in HCC treatment. In this study, we investigated impact ginsenoside RK1 on ferroptosis induction cells and elucidated underlying mechanisms.
Язык: Английский
Процитировано
3
Aging advances., Год журнала: 2024, Номер 1(2), С. 118 - 134
Опубликована: Дек. 1, 2024
Aging is a complex biological process characterized by gradual deterioration in physiological activities, contributing to an elevated risk of different age-associated malignancies, including cancer. The current review paper aims elucidate the association between hallmarks aging and parallel development Various cellular molecular mechanisms underlying such as senescence, genomic instability, telomere shortening play significant role accumulation genetic mutations disruption activities. It has also discussed immune system’s aging, indicating how age-linked dysfunction compromises body’s capacity recognize eradicate pre-tumorous cells, thus promoting cancer advancement. This examines affects tumor microenvironment, where age-related alterations stromal extracellular matrix factors lead conditions that promote growth proliferation. Furthermore, it discusses concept “accelerated aging” survivors, highlighting negative effects its therapy intensify process, resulting greater physical cognitive decline. explores prospect new therapies target aging-related processes ameliorate outcomes. Overall, underscores need for ongoing interdisciplinary research understand relationship cancer, ultimately aiming develop more effective prevention treatment approaches.
Язык: Английский
Процитировано
3
BMC Complementary Medicine and Therapies, Год журнала: 2024, Номер 24(1)
Опубликована: Июль 26, 2024
Abstract Objective To evaluate the anti-tumor effector of Liuwei Dihuang Decoction (LWDHD) in prostate cancer (PCa) and explore potential mechanism using experimental validation, network pharmacology, bioinformatics analysis, molecular docking. Methods CCK test, Clone formation assay wound-healing assays were used to determine effect LWDHD on growth metastasis. The active ingredients targets obtained from TCMSP database, relevant selected by GeneCards, OMIM DisGeNET databases for PCa. cross-targets drugs disease imported into STRING database construct protein interactions. was also visualized Cytoscape software core are screened Network Analyzer plug-in. Gene Ontology (GO) Kyoto Encyclopedia Genes Genomes (KEGG) pathway enrichment analyzed R software. TCGA analyze correlation genes. AutoDock vina predict docking binding ability key targets. Through WB q-PCR experiments, above gene detected verify Results scratch tests confirmed that could inhibit proliferation, invasion migration cells. experiments showed inhibited long-term proliferative capacity PC3 PCa had a total 99 common targets, establishing “drug-ingredient-common target” network. GO KEGG PI3K/AKT, MAPK, TP53 pathway, MYC, TNF other signaling pathways found. Bioinformatics analysis MYC highly expressed CCND1 MAPK1 low tissues. In addition, TP53, AKT1, positively correlated with MAPK1, among which AKT1 most closely MAPK1. Molecular results quercetin, kaempferol, β-sitosterol main treatment combined proteins well. expression PI3K AKT Conclusion therapy is multi-target multi-pathway complex process, may be related biological processes mediated pathways, such as cell proliferation inhibition metastasis, regulation pathways. PI3K/AKT central proliferation.
Язык: Английский
Процитировано
0
Microbial Cell Factories, Год журнала: 2024, Номер 23(1)
Опубликована: Авг. 9, 2024
To effectively introduce plasmids into Bacillus species and conduct genetic manipulations in chassis strains, it is essential to optimize transformation methods. These methods aim extend the period of competence enhance permeability cell membrane facilitate entry exogenous DNA. Although various strategies have been explored, few studies delved identifying metabolites pathways associated with enhanced competence. Additionally, derivative strains non-functional restriction-modification systems demonstrated superior efficiency transforming DNA, lacking more explorations regulation conducted by system process.
Язык: Английский
Процитировано
0