The Alk receptor tyrosine kinase regulates Sparkly, a novel activity regulating neuropeptide precursor in the Drosophila CNS

Опубликована: Май 20, 2024

Numerous roles for the Alk receptor tyrosine kinase have been described in Drosophila , including functions central nervous system (CNS), however molecular details are poorly understood. To gain mechanistic insight, we employed Targeted DamID (TaDa) transcriptional profiling to identify targets of signaling larval CNS. TaDa was CNS tissues, while genetically manipulating output. The resulting data were analysed together with scRNA-seq datasets performed under similar conditions, identifying a role regulation neuroendocrine gene expression. Further integration bulk/scRNA-seq and protein from brains which manipulated, identified previously uncharacterized neuropeptide precursor encoded by CG4577 as an target. named Sparkly (Spar), is expressed subset Alk-positive cells developing CNS, circadian clock neurons. In agreement our analysis, overexpression ligand Jeb resulted increased levels Spar We show that (Clock) neurons, flies lacking exhibit defects sleep activity control. summary, report novel regulating regulated

Язык: Английский

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Intrinsic and damage-induced JAK/STAT signaling regulate developmental timing by the Drosophila prothoracic gland

Disease Models & Mechanisms, Год журнала: 2021, Номер 15(1)

Опубликована: Ноя. 29, 2021

Development involves tightly paced, reproducible sequences of events, yet it must adjust to conditions external it, such as resource availability and organismal damage. A major mediator damage-induced immune responses in vertebrates insects is JAK/STAT signaling. At the same time, activation by Drosophila Upd cytokines pleiotropically involved normal development multiple organs. Whether inflammatory developmental roles intersect unknown. Here, we show that active during prothoracic gland (PG), which controls metamorphosis onset through ecdysone production. Reducing signaling decreased PG size advanced metamorphosis. Conversely, hyperactivation overexpression pathway components or SUMOylation loss caused hypertrophy delay. Tissue damage tumors, known secrete cytokines, also activated delayed metamorphosis, at least part inducing expression target Apontic. signaling, therefore, regulates co-opting its role PG. Our findings provide insights on how systemic effects cancer can interfere with hormonally controlled transitions.

Язык: Английский

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Mammalian puberty: a fly perspective

FEBS Journal, Год журнала: 2022, Номер 290(2), С. 359 - 369

Опубликована: Май 24, 2022

Mammalian puberty and Drosophila metamorphosis, despite their evolutionary distance, exhibit similar design principles conservation of molecular components. In this Viewpoint, we review recent advances in area the similarities between both processes terms signaling pathways neuroendocrine circuits involved. We argue that detection uptake peripheral fat by prothoracic endocrine cells induces endomembrane remodeling ribosomal maturation, leading to acquisition high biosynthetic secretory capacity. The absence fat-neuroendocrine interorgan communication leads giant, obese, non-pupating larvae. Importantly, human leptin is capable pupariation process Drosophila, its expression prevents obesity triggers maturation mutants do not pupate. This implies insect metamorphosis can be used address issues related biology puberty.

Язык: Английский

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Partial reuse of circadian clock genes along parallel clines of diapause in two moth species

Molecular Ecology, Год журнала: 2023, Номер 32(13), С. 3419 - 3439

Опубликована: Март 31, 2023

Abstract Understanding the molecular basis of repeated evolution improves our ability to predict across tree life. Only since last decade has high‐throughput sequencing enabled comparative genome scans thoroughly examine repeatability genetic changes driving phenotypic evolution. The Asian corn borer (ACB), Ostrinia furnacalis (Guenée), and European (ECB), nubilalis (Hübner), are two closely related moths displaying repeatable phenological adaptation a wide range climates on separate continents, largely manifesting as in timing diapause induction termination latitude. Candidate genes underlying variation North American ECB have been previously identified. Here, we sampled seven ACB populations 23 degrees latitude China elucidate evolutionary mechanisms parallel clinal responses species. Using pooled whole‐genome (Pool‐seq) data, population genomic analyses revealed hundreds single nucleotide polymorphisms (SNP) whose allele frequencies covaried with mean phenotypes along cline. Genes involved circadian rhythm were over‐represented among candidate strong signatures spatially varying selection. one clock associated showed evidence reuse (period [ per ]), but alleles not shared between species nor their outgroup, implicating independent mutational paths. Nonetheless, adaptive introgression was discovered at putative loci located elsewhere genome, suggesting that de novo mutations might both underlie

Язык: Английский

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The macrophage genetic cassette inr/dtor/pvf2 is a nutritional status checkpoint for developmental timing

Science Advances, Год журнала: 2023, Номер 9(38)

Опубликована: Сен. 20, 2023

A small number of signaling molecules, used reiteratively, control differentiation programs, but the mechanisms that adapt developmental timing to environmental cues are less understood. We report here a macrophage inr/dtor/pvf2 genetic cassette is checkpoint in Drosophila, which either licenses or delays biosynthesis steroid hormone endocrine gland and metamorphosis according larval nutritional status. Insulin receptor/dTor macrophages required sufficient for production PDGF/VEGF family growth factor Pvf2, turns on transcription sterol Halloween genes prothoracic via its receptor Pvr. In response starvation event manipulation, low Pvf2 signal until it becomes Pvr-independent, thereby prolonging before pupariation. The significance this host fitness illustrated by observation regulates size pupae adult flies.

Язык: Английский

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