Tumour Virus Research,
Год журнала:
2023,
Номер
15, С. 200260 - 200260
Опубликована: Май 9, 2023
The
tegument
is
the
structure
between
envelope
and
nucleocapsid
of
herpesvirus
particles.
Viral
(and
cellular)
proteins
accumulate
to
create
layers
tegument.
Some
Epstein-Barr
virus
(EBV)
are
conserved
widely
in
Herpesviridae,
but
others
shared
only
by
members
gamma-herpesvirus
subfamily.
As
interface
nucleocapsid,
functions
virion
morphogenesis
budding
during
progeny
production.
When
a
particle
enters
cell,
enzymes
such
as
kinase
deubiquitinase,
transcriptional
activators
released
from
promote
infection.
Moreover,
some
EBV
involved
oncogenesis.
Here,
we
summarize
roles
proteins,
comparison
those
other
herpesviruses.
Abstract
Epstein-Barr
virus
(EBV),
a
γ-herpesvirus,
is
the
first
identified
oncogenic
virus,
which
establishes
permanent
infection
in
humans.
EBV
causes
infectious
mononucleosis
and
also
tightly
linked
to
many
malignant
diseases.
Various
vaccine
formulations
underwent
testing
different
animals
or
However,
none
of
them
was
able
prevent
no
has
been
approved
date.
Current
efforts
focus
on
antigen
selection,
combination,
design
improve
efficacy
vaccines.
glycoproteins
such
as
gH/gL,
gp42,
gB
show
excellent
immunogenicity
preclinical
studies
compared
previously
favored
gp350
antigen.
Combinations
multiple
proteins
various
designs
become
more
attractive
approaches
considering
complex
life
cycle
complicated
mechanisms
EBV.
Besides,
rationally
designed
vaccines
virus-like
particles
(VLPs)
protein
scaffold-based
elicited
potent
immune
responses
than
soluble
antigens.
In
addition,
humanized
mice,
rabbits,
well
nonhuman
primates
that
can
be
infected
by
significantly
aid
development.
Innovative
approaches,
including
polymer-based
nanoparticles,
development
effective
adjuvants,
antibody-guided
design,
will
further
enhance
candidates.
this
review,
we
summarize
(i)
disease
burden
caused
necessity
developing
an
vaccine;
(ii)
previous
available
animal
models;
(iii)
future
trends
vaccines,
activation
cellular
responses,
novel
immunogen
heterologous
prime-boost
approach,
induction
mucosal
immunity,
application
nanoparticle
delivery
system,
modern
adjuvant
Molecular Cell,
Год журнала:
2023,
Номер
83(13), С. 2367 - 2386.e15
Опубликована: Июнь 12, 2023
Epstein-Barr
virus
(EBV)
causes
infectious
mononucleosis,
triggers
multiple
sclerosis,
and
is
associated
with
200,000
cancers/year.
EBV
colonizes
the
human
B
cell
compartment
periodically
reactivates,
inducing
expression
of
80
viral
proteins.
However,
much
remains
unknown
about
how
remodels
host
cells
dismantles
key
antiviral
responses.
We
therefore
created
a
map
EBV-host
EBV-EBV
interactions
in
undergoing
replication,
uncovering
conserved
herpesvirus
versus
EBV-specific
targets.
The
EBV-encoded
G-protein-coupled
receptor
BILF1
MAVS
UFM1
E3
ligase
UFL1.
Although
UFMylation
14-3-3
proteins
drives
RIG-I/MAVS
signaling,
BILF1-directed
instead
triggered
packaging
into
mitochondrial-derived
vesicles
lysosomal
proteolysis.
In
absence
BILF1,
replication
activated
NLRP3
inflammasome,
which
impaired
pyroptosis.
Our
results
provide
protein
interaction
network
resource,
reveal
UFM1-dependent
pathway
for
selective
degradation
mitochondrial
cargo,
highlight
as
novel
therapeutic
target.
Nucleic Acids Research,
Год журнала:
2024,
Номер
52(5), С. 2112 - 2129
Опубликована: Фев. 2, 2024
Abstract
High-level
folding
of
chromatin
is
a
key
determinant
the
shape
and
functional
state
chromosomes.
During
cell
division,
structural
maintenance
chromosome
(SMC)
complexes
such
as
condensin
cohesin
ensure
large-scale
into
visible
In
contrast,
SMC5/6
complex
plays
more
local
context-specific
roles
in
organization
interphase
chromosomes
with
important
implications
for
health
disease.
Recent
advances
single-molecule
biophysics
cryo-electron
microscopy
revealed
insights
architecture
how
interactions
connecting
to
components
give
rise
its
unique
repertoire
functions.
this
review,
we
provide
an
integrative
view
features
that
differentiates
from
other
SMC
enzymes
these
enable
dramatic
reorganization
DNA
space
during
repair
reactions
genome
transactions.
Finally,
explore
mechanistic
basis
dynamic
targeting
damaged
crucial
role
human
health.
PLoS Pathogens,
Год журнала:
2024,
Номер
20(2), С. e1011954 - e1011954
Опубликована: Фев. 1, 2024
Epstein-Barr
virus
(EBV)
is
a
ubiquitous
human
lymphotropic
herpesvirus
that
causally
associated
with
several
malignancies.
In
addition
to
latent
factors,
lytic
replication
contributes
cancer
development.
this
study,
we
examined
whether
the
gene
BNRF1,
which
conserved
among
gamma-herpesviruses,
has
an
important
role
in
lymphomagenesis.
We
found
lymphoblastoid
cell
lines
(LCLs)
established
by
BNRF1-knockout
EBV
exhibited
remarkably
lower
pathogenicity
mice
xenograft
model
than
LCLs
produced
wild-type
(LCLs-WT).
RNA-seq
analyses
revealed
BNRF1
elicited
expression
of
interferon-inducible
protein
27
(IFI27),
promotes
proliferation.
IFI27
knockdown
LCLs-WT
resulted
excessive
production
reactive
oxygen
species,
leading
death
and
significantly
decreased
their
vivo
.
also
confirmed
was
upregulated
during
primary
infection
B-cells.
Our
findings
promoted
robust
proliferation
B-cells
were
transformed
via
upregulation
both
vitro
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Фев. 17, 2024
Structural
maintenance
of
chromosomes
(SMC)
protein
complexes
play
pivotal
roles
in
genome
organization
and
across
all
domains
life.
In
prokaryotes,
SMC
family
Wadjet
structurally
resemble
the
widespread
MukBEF
genome-organizing
but
serve
a
defensive
role
by
inhibiting
plasmid
transformation.
We
previously
showed
that
specifically
cleaves
circular
DNA;
however,
molecular
mechanism
underlying
DNA
substrate
recognition
remains
unclear.
Here,
we
use
vitro
single-molecule
imaging
to
directly
visualize
loop
extrusion
cleavage
Wadjet.
find
is
symmetric
extruder
simultaneously
reels
from
both
sides
growing
this
activity
requires
dimeric
JetABC
supercomplex
containing
two
dimers
JetC
motor
subunit.
On
surface-anchored
DNAs,
extrudes
full
length
44
kilobase
pair
plasmid,
stalls,
then
DNA.
Our
findings
reveal
specific
elimination
plasmids
Wadjet,
establish
as
an
evolutionarily
conserved
among
kingdoms
Nature Communications,
Год журнала:
2023,
Номер
14(1)
Опубликована: Сен. 20, 2023
Abstract
Nuclear
pore
complexes
(NPCs)
have
increasingly
recognized
interactions
with
the
genome,
as
exemplified
in
yeast,
where
they
bind
transcribed
or
damaged
chromatin.
By
combining
genome-wide
approaches
live
imaging
of
model
loci,
we
uncover
a
correlation
between
NPC
association
and
accumulation
R-loops,
which
are
genotoxic
structures
formed
through
hybridization
nascent
RNAs
their
DNA
templates.
Manipulating
hybrid
formation
demonstrates
that
R-loop
per
se,
rather
than
transcription
R-loop-dependent
damages,
is
primary
trigger
for
relocation
to
NPCs.
Mechanistically,
repositioning
involves
recognition
by
ssDNA-binding
protein
RPA,
SUMO-dependent
NPC-associated
factors.
Preventing
leads
lethality
hybrid-accumulating
conditions,
while
tethering
hybrid-prone
locus
attenuates
genetic
instability.
Remarkably,
this
pathway
molecular
factors
similar
those
required
stalled
replication
forks
NPCs,
supporting
existence
convergent
mechanisms
sensing
transcriptional
stresses.
