The assembly-defective phenotype of an FIV Gag polyprotein containing the SIV nucleocapsid zinc finger motifs is reversed by including the SIV SP2 domain in the chimeric protein

Virology, Год журнала: 2024, Номер 597, С. 110163 - 110163

Опубликована: Июнь 29, 2024

Язык: Английский

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Influence of HIV-1 genomic RNA on the formation of Gag biomolecular condensates

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Фев. 23, 2023

Biomolecular condensates (BMCs) play an important role in the replication of a growing number viruses, but many mechanistic details remain to be elucidated. Previously, we demonstrated that pan-retroviral nucleocapsid (NC) and HIV-1 pr55 Gag (Gag) proteins phase separate into condensates, protease (PR)-mediated maturation Gag-Pol precursor yield self-assembling BMCs having core architecture. Using biochemical imaging techniques, aimed further characterize separation by determining which its intrinsically disordered regions (IDRs) influence formation how viral genomic RNA (gRNA) could BMC abundance size. We found mutations matrix (MA) domain or NC zinc finger motifs altered condensate size salt-dependent manner. were also bimodally influenced gRNA, with condensate-promoting regime at lower protein concentrations gel dissolution higher concentrations. Interestingly, incubation CD4 + T cell nuclear lysates led larger as compared much smaller ones observed presence cytoplasmic lysates. These findings suggests composition properties Gag-containing may differential association host factors cytosolic compartments during virus assembly. This study significantly advances our understanding provides foundation for future therapeutic targeting virion

Язык: Английский

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Methodological approaches to studying phase separation and HIV-1 replication: Current and future perspectives

Methods, Год журнала: 2024, Номер 229, С. 147 - 155

Опубликована: Сен. 1, 2024

Язык: Английский

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Experimental Considerations for the Evaluation of Viral Biomolecular Condensates

Annual Review of Virology, Год журнала: 2024, Номер 11(1), С. 105 - 124

Опубликована: Сен. 26, 2024

Biomolecular condensates are nonmembrane-bound assemblies of biological polymers such as protein and nucleic acids. An increasingly accepted paradigm across the viral tree life is (a) that viruses form biomolecular (b) formation required for virus. Condensates can promote replication by promoting packaging, genome compaction, membrane bending, co-opting host translation. This review primarily concerned with exploring methodologies assessing virally encoded condensates. The goal this to provide an experimental framework virologists consider when designing experiments identify their components, reconstitute condensation cell free from minimal (c) ask questions about what conditions lead condensation, (d) map these back cycle, (e) design test inhibitors/modulators potential therapeutics. attempts integrate virology, biology, biochemistry approaches.

Язык: Английский

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The life cycle of feline immunodeficiency virus

Virology, Год журнала: 2024, Номер 601, С. 110304 - 110304

Опубликована: Ноя. 16, 2024

Язык: Английский

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