bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2023,
Номер
unknown
Опубликована: Ноя. 11, 2023
Abstract
In
recent
years,
there
has
been
a
growing
interest
in
understanding
the
function
of
claustrum.
The
claustrum
is
thin,
long,
subcortical
structure
with
dense
connections
to
cortex.
Despite
these
extensive
connections,
manner
which
influences
broader
cortical
activity
remains
unclear.
We
used
mesoscale
calcium
fluorescence
imaging
examine
resting
state
(1-5Hz)
and
sensory-evoked
responses
lightly
anesthetized
mice,
before
after
bi-directional
chemogenetic
modulation
claustrum-prefrontal
neurons.
Claustrum
inhibition
resulted
increased
anterior-medial
areas,
whereas
excitation
decreased
regions.
also
led
an
local
coupling
between
areas
frontal
cortex,
correlation
anterior
medial
regions-of-interest
(ROIs)
lateral
posterior
ROIs,
sensory
evoked
response
visual
These
results
are
consistent
finding
that
large
feed-forward
inhibitory
effect
on
PFC,
concept
specific
claustrocortical
pathways
may
modulate
recruit
synchronize
topographically
distinct
modules.
Together
show
neural
modulates
excitability
prefrontal
networks,
suggesting
potential
target
for
prefrontal-dependent
behaviors
such
as
learning,
attention,
stress
regulation.
Cell Reports,
Год журнала:
2023,
Номер
42(2), С. 112118 - 112118
Опубликована: Фев. 1, 2023
The
claustrum
(CLA)
is
a
conspicuous
subcortical
structure
interconnected
with
cortical
and
regions.
Its
regional
anatomy
cell-type-specific
connections
in
the
mouse
remain
not
fully
determined.
Using
multimodal
reference
datasets,
we
confirmed
delineation
of
CLA
as
single
group
neurons
embedded
agranular
insular
cortex.
We
quantitatively
investigated
brain-wide
inputs
outputs
using
bulk
anterograde
retrograde
viral
tracing
data
neuron
data.
found
that
prefrontal
module
has
more
cell
types
projecting
to
than
other
modules,
layer
5
IT
predominating.
nine
morphological
principal
topographically
innervate
functionally
linked
targets,
preferentially
midline
areas,
secondary
motor
area,
entorhinal
area.
Together,
this
study
provides
detailed
wiring
diagram
CLA,
laying
foundation
for
studying
its
functions
at
cellular
level.
PLoS ONE,
Год журнала:
2024,
Номер
19(4), С. e0298349 - e0298349
Опубликована: Апрель 18, 2024
The
claustrum
is
an
irregular
and
fine
sheet
of
grey
matter
in
the
basolateral
telencephalon
present
almost
all
mammals.
has
been
object
several
studies
using
animal
models
and,
more
recently,
human
beings
neuroimaging.
One
most
extended
cognitive
processes
attributed
to
salience
process,
which
also
related
insular
cortex.
In
same
way,
with
subjects
functional
magnetic
resonance
imaging
have
reported
coactivation
claustrum/insular
cortex
integration
sensory
signals.
This
left
or
right
asymmetry
task
literature
neurological
disorders
such
as
Alzheimer's
disease
schizophrenia,
relating
severity
delusions
reduction
claustral
volume.
We
a
connectivity
study
claustrum.
Resting-state
anatomical
MRI
data
from
100
healthy
were
analyzed;
taken
Human
Connectome
Project
(HCP,
NIH
Blueprint:
Project),
2x2x2
mm3
voxel
resolution.
hypothesize
that
1)
node
involved
different
brain
networks,
2)
pattern
cortex's
pattern,
3)
claustrum's
connectivity.
Our
findings
include
at
least
three
networks
found
between
claustrum,
frontoparietal
network,
default
mode
network
distinctive
attribute.
dorsal
attention
supports
hypothesis
asymmetry.
These
provide
evidence,
suggesting
coupled
serving
together
instantiate
new
states
by
flexibly
modulating
interacting
other
control
processing
networks.
Nature Communications,
Год журнала:
2024,
Номер
15(1)
Опубликована: Июнь 26, 2024
Abstract
The
claustrum
has
been
linked
to
attention
and
sleep.
We
hypothesized
that
this
reflects
a
shared
function,
determining
responsiveness
stimuli,
which
spans
the
axis
of
engagement.
To
test
hypothesis,
we
recorded
population
dynamics
from
male
mice
during
both
sleep
an
attentional
task
(‘ENGAGE’).
Heightened
activity
in
neurons
projecting
anterior
cingulate
cortex
(ACCp)
corresponded
reduced
sensory
Similarly,
ENGAGE
task,
heightened
ACCp
correlated
with
disengagement
behavioral
lapses,
while
low
hyper-engagement
impulsive
errors.
Chemogenetic
elevation
awakenings
errors
task.
Furthermore,
employing
exploration
strategy
showed
stronger
correlation
between
performance
compared
exploitation
complexity.
Our
results
implicate
restricting
engagement
goal-directed
behavior.
Abstract
The
anatomical
organization
of
the
rodent
claustrum
remains
obscure
due
to
lack
clear
borders
that
distinguish
it
from
neighboring
forebrain
structures.
Defining
what
constitutes
is
imperative
for
elucidating
its
functions.
Methods
based
on
gene/protein
expression
or
transgenic
mice
have
been
used
spatially
outline
but
often
report
incomplete
labeling
and/or
specificity
during
certain
neurodevelopmental
timepoints.
To
reliably
identify
projection
cells
in
mice,
we
propose
a
simple
immunolabelling
method
juxtaposes
pattern
claustrum-enriched
and
cortical-enriched
markers.
We
determined
immunoreactive
markers
Nurr1
Nr2f2
are
devoid
cortical
marker
Tle4,
which
allowed
us
differentiate
adjoining
cells.
Using
retrograde
tracing,
verified
nearly
all
neurons
Tle4
expressed
Nurr1/Nr2f2
different
degrees.
At
neonatal
stages
between
7
21
days,
were
identified
by
their
Nurr1-postive/Tle4-negative
profile,
time-period
when
other
techniques
localize
adult
ineffective.
Finally,
exposure
environmental
novelty
enhanced
neuronal
activation
c-Fos
region.
Notably,
was
mainly
restricted
Nurr1-positive
absent
Tle4-positive
cells,
thus
corroborating
previous
work
reporting
novelty-induced
activation.
Taken
together,
this
will
aid
studying
postnatal
development
may
improve
histological
functional
studies
where
approaches
not
amenable.
Nature Communications,
Год журнала:
2024,
Номер
15(1)
Опубликована: Окт. 17, 2024
Slow
waves
are
a
distinguishing
feature
of
non-rapid-eye-movement
(NREM)
sleep,
an
evolutionarily
conserved
process
critical
for
brain
function.
Non-human
studies
suggest
that
the
claustrum,
small
subcortical
nucleus,
coordinates
slow
waves.
We
show
that,
in
contrast
to
neurons
from
other
regions,
claustrum
human
increase
their
spiking
activity
and
track
during
NREM
suggesting
plays
role
coordinating
sleep
architecture.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2023,
Номер
unknown
Опубликована: Март 24, 2023
Abstract
The
claustrum
(CLA)
is
a
brain
nucleus
located
between
the
insula
and
lateral
striatum,
implicated
in
wide
range
of
behaviors.
Underpinning
different
behavioral
phenotypes
connectivity
various
cortical
regions,
including
anterior
cingulate
cortex
(ACC).
CLA
projection
neurons
are
glutamatergic
neurons,
however,
impact
on
its
targets
has
been
shown
some
studies
to
be
inhibitory.
Such
inhibition
likely
arise
from
claustral
activation
interneurons,
intricate
synaptic
cell
types
not
known.
Here,
we
combine
vivo
ex
electrophysiology
optogenetics
reveal
functional
organization
CLA-ACC
pathway
according
identity
pre-
postsynaptic
populations.
Optogenetic
stimulation
awake
mice
resulted
multiphasic
excitatory
inhibitory
responses
ACC
cells,
which
depended
layer,
type,
stimulated
population.
Using
paired
recordings
ACC,
monosynaptic
were
recorded
pyramidal
cells
interneurons
following
photostimulation
terminals.
axons
formed
connections
all
layers,
but
probability
strength
type
projection,
target
layer
neuron.
This
may
explain
complex
other
thus
resolving
discrepancies
field
shedding
light
role
plays
function.
