Eye-specific synaptic clustering through activity-dependent stabilization and punishment mechanisms in the developing visual system DOI Open Access
Chenghang Zhang, Colenso M. Speer

Опубликована: Окт. 23, 2023

Co-active synaptic connections are often spatially clustered to enable local dendritic computations underlying learning, memory, and basic sensory processing. In the mammalian visual system, retinal ganglion cell (RGC) axons converge form inputs for signal integration in dorsal lateral geniculate nucleus (dLGN) of thalamus. Retinogeniculate synapse clustering is promoted by experience after eye-opening, but earliest events cluster formation potential regulation spontaneous wave activity prior unknown. Here, using volumetric super-resolution single-molecule localization microscopy together with eye-specific labeling developing retinogeniculate synapses mouse, we show that activity-dependent during first postnatal week. We identified a subset complex larger presynaptic vesicle pools multiple active zones simultaneously promote like-eye (synaptic stabilization) prohibit from opposite eye punishment). mutant mice disrupted activity, form, fail drive punishment seen controls. These results highlight role regulating stabilization signals contributing circuits essential perception behavior.

Язык: Английский

Microanalytical Mass Spectrometry with Super-Resolution Microscopy Reveals a Proteome Transition During Development of the Brain’s Circadian Pacemaker DOI
Sam B. Choi, Tarlan Vatan, Theresa Alexander

и другие.

Analytical Chemistry, Год журнала: 2023, Номер 95(41), С. 15208 - 15216

Опубликована: Окт. 4, 2023

During brain development, neuronal proteomes are regulated in part by changes spontaneous and sensory-driven activity immature neural circuits. A longstanding model for studying activity-dependent circuit refinement is the developing mouse visual system where formation of axonal projections from eyes to influenced retinal prior onset vision experience after eye-opening. The precise proteomic retinorecipient targets that occur during this developmental transition unknown. Here, we developed a microanalytical proteomics pipeline using capillary electrophoresis (CE) electrospray ionization (ESI) mass spectrometry (MS) discovery setting quantify chief circadian pacemaker, suprachiasmatic nucleus (SCN), before photoreceptor-dependent function. Nesting CE-ESI with trapped ion mobility time-of-flight (TOF) (TimsTOF PRO) doubled number identified quantified proteins compared TOF-only control on same analytical platform. From 10 ng peptide input, corresponding <∼0.5% total local tissue proteome, technical triplicate analyses 1894 1066 proteins, including many important canonical functions axon guidance, synapse function, glial cell maturation, extracellular matrix refinement. Label-free quantification revealed differential regulation 166 over enrichment guidance-associated eye-opening synapse-associated protein Super-resolution imaging select STochastic Optical Reconstruction Microscopy (STORM) corroborated MS results showed increased presynaptic abundance pre/post SCN reflects increase number, but not size or extrasynaptic expression. This work marks first development systematic application TimsTOF PRO CE-ESI-based microproteomics integration volumetric super-resolution STORM expand repertoire technologies supporting neuroscience.

Язык: Английский

Процитировано

7
Volumetric super-resolution imaging by serial ultrasectioning and stochastic optical reconstruction microscopy in mouse neural tissue DOI Creative Commons
Tarlan Vatan,

Jacqueline Minehart,

Chenghang Zhang

и другие.

STAR Protocols, Год журнала: 2021, Номер 2(4), С. 100971 - 100971

Опубликована: Ноя. 24, 2021

Here, we present a protocol for collecting large-volume, four-color, single-molecule localization imaging data from neural tissue. We have applied this technique to map the location and identities of chemical synapses across whole cells in mouse retinae. Our sample preparation approach improves 3D STORM image quality by reducing tissue scattering, photobleaching, optical distortions associated with deep imaging. This can be extended use on other types enabling life scientists perform volumetric super-resolution diverse biological models. For complete details execution protocol, please refer Sigal et al. (2015).

Язык: Английский

Процитировано

7
Activity-dependent synapse clustering underlies eye-specific competition in the developing retinogeniculate system DOI Open Access
Chenghang Zhang, Tarlan Vatan, Colenso M. Speer

и другие.

Опубликована: Дек. 16, 2024

Co-active synaptic connections are often spatially clustered to facilitate local dendritic computations underlying learning, memory, and basic sensory processing. In the mammalian visual system, retinal ganglion cell (RGC) axons converge form inputs that enable signal integration in dorsal lateral geniculate nucleus (dLGN) of thalamus. While experience promotes retinogeniculate synapse clustering after eye-opening, earliest events cluster formation prior unknown. Here, using volumetric super-resolution single-molecule localization microscopy eye-specific labeling developing synapses mice, we show is activity-dependent during refinement first postnatal week. We identified a subset with multiple active zones surrounded by like-eye depleted from opposite eye. mutant mice disrupted spontaneous wave activity, still form, but do not exhibit seen controls. These results highlight role for activity regulating circuits essential perception behavior.

Язык: Английский

Процитировано

0
Eye-specific synaptic clustering through activity-dependent stabilization and punishment mechanisms in the developing visual system DOI Creative Commons
Chenghang Zhang, Tarlan Vatan, Colenso M. Speer

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Сен. 29, 2023

Abstract Co-active synaptic connections are often spatially clustered to facilitate local dendritic computations underlying learning, memory, and basic sensory processing. In the mammalian visual system, retinal ganglion cell (RGC) axons converge form inputs that enable signal integration in dorsal lateral geniculate nucleus (dLGN) of thalamus. While experience promotes retinogeniculate synapse clustering after eye-opening, earliest events cluster formation prior unknown. Here, using volumetric super-resolution single-molecule localization microscopy eye-specific labeling developing synapses mice, we show is activity-dependent during refinement first postnatal week. We identified a subset with multiple active zones surrounded by like-eye depleted from opposite eye. mutant mice disrupted spontaneous wave activity, still form, but do not exhibit seen controls. These results highlight role for activity regulating circuits essential perception behavior.

Язык: Английский

Процитировано

0
Eye-specific synaptic clustering through activity-dependent stabilization and punishment mechanisms in the developing visual system DOI Open Access
Chenghang Zhang, Colenso M. Speer

Опубликована: Окт. 23, 2023

Co-active synaptic connections are often spatially clustered to enable local dendritic computations underlying learning, memory, and basic sensory processing. In the mammalian visual system, retinal ganglion cell (RGC) axons converge form inputs for signal integration in dorsal lateral geniculate nucleus (dLGN) of thalamus. Retinogeniculate synapse clustering is promoted by experience after eye-opening, but earliest events cluster formation potential regulation spontaneous wave activity prior unknown. Here, using volumetric super-resolution single-molecule localization microscopy together with eye-specific labeling developing retinogeniculate synapses mouse, we show that activity-dependent during first postnatal week. We identified a subset complex larger presynaptic vesicle pools multiple active zones simultaneously promote like-eye (synaptic stabilization) prohibit from opposite eye punishment). mutant mice disrupted activity, form, fail drive punishment seen controls. These results highlight role regulating stabilization signals contributing circuits essential perception behavior.

Язык: Английский

Процитировано

0
Eye-specific synaptic clustering through activity-dependent stabilization and punishment mechanisms in the developing visual system DOI Open Access
Chenghang Zhang, Colenso M. Speer

Опубликована: Окт. 23, 2023

Co-active synaptic connections are often spatially clustered to enable local dendritic computations underlying learning, memory, and basic sensory processing. In the mammalian visual system, retinal ganglion cell (RGC) axons converge form inputs for signal integration in dorsal lateral geniculate nucleus (dLGN) of thalamus. Retinogeniculate synapse clustering is promoted by experience after eye-opening, but earliest events cluster formation potential regulation spontaneous wave activity prior unknown. Here, using volumetric super-resolution single-molecule localization microscopy together with eye-specific labeling developing retinogeniculate synapses mouse, we show that activity-dependent during first postnatal week. We identified a subset complex larger presynaptic vesicle pools multiple active zones simultaneously promote like-eye (synaptic stabilization) prohibit from opposite eye punishment). mutant mice disrupted activity, form, fail drive punishment seen controls. These results highlight role regulating stabilization signals contributing circuits essential perception behavior.

Язык: Английский

Процитировано

0