The Journal of Steroid Biochemistry and Molecular Biology, Год журнала: 2024, Номер 245, С. 106634 - 106634
Опубликована: Ноя. 16, 2024
Язык: Английский
Nature Cell Biology, Год журнала: 2024, Номер 26(7), С. 1093 - 1109
Опубликована: Июнь 17, 2024
Язык: Английский
Процитировано
20
Frontiers in Microbiology, Год журнала: 2025, Номер 15
Опубликована: Янв. 7, 2025
Despite the advantages of endoscopic surgery in reducing trauma and enhancing recovery for breast cancer patients, its impact on gut microbiota, which is crucial health estrogen metabolism, remains unclear. Further investigation necessary to fully understand this implications. Between June December 2022, fecal samples were collected from 20 patients who underwent surgery. The microbiota composition was determined using 16S rRNA sequencing, while metabolites analyzed through liquid chromatography-tandem mass spectrometry (LC-MS/MS). Bioinformatics statistical analyses employed identify significant alterations microbial taxa abundance assess intergroup differences. These included t-tests pairwise comparisons, one-way ANOVA multiple group chi-square tests categorical data analysis. Endoscopic subtly changed diversity composition. Post-surgery, there a reduction Lachnospiraceae, Monoglobaceae Firmicutes Bacteroides ratios. Shifts also observed, impacted pathways such as primary bile biosynthesis Ascorbate aldarate with PE(PGD1/18:1(9Z)) identified key differential metabolite that increased post-surgery. Azasetron, tyramine glucuronide, DL-DOPA, phthalide, acetophenazine, aciclovir, creatinine bicarbonate, 4-oxo-L-proline being associated distinct bacterial taxa. Breast undergoing experience shift their metabolic profiles. Therefore, postoperative management, particular focus adjustment patient outcomes.
Язык: Английский
Процитировано
0
Biochemical Society Transactions, Год журнала: 2025, Номер 00(00)
Опубликована: Март 26, 2025
Tetraspanins are a family of 33 proteins in mammals believed to play crucial role the compartmentalization various associated within cells and membranes. Recent studies have elucidated structure several tetraspanin members, revealing that while four transmembrane domains typically adopt cone-shaped configuration crystals, other conformations also possible. This may explain why tetraspanins often enriched curved tubular cellular structures, such as microvilli, tunneling nanotubes, retraction fibers, or at site virus budding, contribute formation maintenance these structures. been detected on midbody remnants migrasomes, well extracellular vesicles (EVs), for which CD9, CD81, CD63 widely used markers. Although their impact certain membrane structures ability regulate function trafficking would suggest potential either EV regulating protein composition, both, efforts characterize roles complicated by conflicting results. In line with interaction cholesterol, two recent suggested presence organization oxysterols cholesterol EVs be regulated Tspan6 CD63, respectively, paving way further research influence lipid composition EVs.
Язык: Английский
Процитировано
0
Frontiers in Oncology, Год журнала: 2025, Номер 15
Опубликована: Апрель 9, 2025
In many cancers, the tumor microenvironment is enriched with cholesterol due to increased biosynthesis and uptake by cancer cells, resulting in accumulation of cholesterol, esters, oxysterols other metabolites various functions. These molecules serve as structural components, energy sources intracellular signaling mediators, while their toxic by-products are secreted suppress anti-tumor immune activity prevent lipid peroxidation that could induce cell apoptosis. Immune cells also contribute dynamics. Tumor-associated macrophages (TAMs) release support metabolism, myeloid-derived suppressor (MDSCs) consume essential such L-arginine, which impairs T-cell proliferation activation. Elevated dendritic migration antigen presentation and, lymphocytes, favors development a regulatory T (Treg) phenotype inhibits antitumor cytokines, further weakening response. findings suggest targeting metabolism promising strategy for treatment, improving efficacy checkpoint blockade (ICB) therapies. this manuscript, molecular mechanisms underlying effects on landscape reviewed potential cholesterol-lowering drugs enhance responses explored.
Язык: Английский
Процитировано
0
Extracellular Vesicles and Circulating Nucleic Acids, Год журнала: 2024, Номер 5(1), С. 95 - 113
Опубликована: Фев. 26, 2024
Язык: Английский
Процитировано
1
Fundamental Research, Год журнала: 2024, Номер 4(4), С. 761 - 769
Опубликована: Март 22, 2024
The genome-wide association study (GWAS) aims to detect associations between individual single nucleotide polymorphisms (SNPs) or SNP interactions and phenotypes decipher the genetic mechanism. Existing GWAS analysis tools have different focuses advantages, but suffer a series of tedious heterogeneous configurations for computation. It is inconvenient researchers simply choose apply these tools, statistically biologically analyze their results usages. To address issues, we develop user friendly web pipeline GWASTool detecting associations, which includes simulation data generation, associated loci detection, result visualization, comparison. provides unified plugin-able framework encapsulate heterogeneity algorithms, simplifies steps energizes tasks. implemented in Java freely available public use at http://www.sdu-idea.cn/GWASTool. website hosts comprehensive collection resources, including manual, description integrated examples standalone version download.
Язык: Английский
Процитировано
1
STAR Protocols, Год журнала: 2024, Номер 5(2), С. 102956 - 102956
Опубликована: Март 21, 2024
Preclinical tumor models have advanced our understanding of the microenvironment. However, temporal dynamics cellular recruitment and retention within these is poorly understood. Here, we present a protocol using transcutaneous labeling compartment subcutaneous orthotopic tumors. We describe process cell line implantation photoconversion tumors to differentiate newly recruited cells from those retained Photoconversion enables tracking both immune egress lymphatics. For complete details on use execution this protocol, please refer Li et al.
Язык: Английский
Процитировано
1
Cancer Medicine, Год журнала: 2024, Номер 13(14)
Опубликована: Июль 1, 2024
The tetraspanin (TSPAN) family comprises 33 membrane receptors involved in various physiological processes humans. Tetrasapanins are surface proteins expressed cells of organisms. They localised to the cell by four transmembrane domains (TM4SF). These bind several and signalling tetraspanin-enriched lipid microdomains (TERM or TEM). Tetraspanins play a critical role anchoring many proteins. also act as scaffold for
Язык: Английский
Процитировано
1
Advances in experimental medicine and biology, Год журнала: 2023, Номер unknown, С. 307 - 336
Опубликована: Дек. 1, 2023
Язык: Английский
Процитировано
1
Опубликована: Янв. 1, 2024
Cervical cancer, a major global health issue and the fourth most common cancer among women, is strongly linked to Human Papillomavirus (HPV) infection. Emerging evidence indicates that oxidative stress plays critical role in carcinogenesis of cervical tissue. This study investigates relationship between markers—specifically oxysterols, lipid oxidation, protein carbonylation—and progression neoplasia. Oxysterols, which are elevated various inflammatory diseases cancers, were measured cervicovaginal fluid samples using LC-MS/MS. The targeted oxysterols included 27-hydroxycholesterol (27-OHC), 7β-hydroxycholesterol (7β-OHC), 7-ketocholesterol (7-KC), 7α,27-dihydroxycholesterol (7α,27-diOHC). Among these, was significantly increased correlation with severity neoplastic stages. In parallel, carbonylation, an indicator cellular stress, assessed. Results revealed higher levels carbonylation compared non-neoplastic controls. These modifications further analysed through redox proteomics identify specific proteins affected. demonstrates oxidation secretions development cancer. markers can potentially serve as early indicators oncogenesis. Identifying these biomarkers may improve screening strategies, enabling identification individuals at risk for neoplasia guiding timely interventions.
Язык: Английский
Процитировано
0