Sde Proteins Coordinate Ubiquitin Utilization and Phosphoribosylation to Establish and Maintain theLegionellaReplication Vacuole DOI Creative Commons

Kristin M. Kotewicz,

Mengyun Zhang, Seongok Kim

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Сен. 7, 2023

Abstract The Legionella pneumophila Sde family of translocated proteins promotes host tubular endoplasmic reticulum (ER) rearrangements that are tightly linked to phosphoribosyl-ubiquitin (pR-Ub) modification Reticulon 4 (Rtn4). have two additional activities unclear relevance the infection process: K63 linkage-specific deubiquitination and phosphoribosyl polyubiquitin (pR-Ub). We show here activity (DUB) stimulates ER while pR-Ub protects replication vacuole from cytosolic surveillance by autophagy. Loss DUB is lowered Rtn4, consistent with fueling production pR-Ub-Rtn4. In parallel, polyUb, in a region protein known as isoleucine patch, prevents binding autophagy adapter p62. An inability mutants modify polyUb results immediate p62 association, critical precursor autophagic attack. ability WT block association decays quickly after bacterial infection, predicted presence previously characterized L. effectors inactivate remove polyUb. sum, these accessory act stimulate protect innate immune sensing temporal fashion.

Язык: Английский

Sde Proteins Coordinate Ubiquitin Utilization and Phosphoribosylation to Establish and Maintain theLegionellaReplication Vacuole DOI Creative Commons

Kristin M. Kotewicz,

Mengyun Zhang, Seongok Kim

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Сен. 7, 2023

Abstract The Legionella pneumophila Sde family of translocated proteins promotes host tubular endoplasmic reticulum (ER) rearrangements that are tightly linked to phosphoribosyl-ubiquitin (pR-Ub) modification Reticulon 4 (Rtn4). have two additional activities unclear relevance the infection process: K63 linkage-specific deubiquitination and phosphoribosyl polyubiquitin (pR-Ub). We show here activity (DUB) stimulates ER while pR-Ub protects replication vacuole from cytosolic surveillance by autophagy. Loss DUB is lowered Rtn4, consistent with fueling production pR-Ub-Rtn4. In parallel, polyUb, in a region protein known as isoleucine patch, prevents binding autophagy adapter p62. An inability mutants modify polyUb results immediate p62 association, critical precursor autophagic attack. ability WT block association decays quickly after bacterial infection, predicted presence previously characterized L. effectors inactivate remove polyUb. sum, these accessory act stimulate protect innate immune sensing temporal fashion.

Язык: Английский

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