Autophagosome development and chloroplast segmentation occur synchronously for piecemeal degradation of chloroplasts DOI Open Access
Masanori Izumi, Sakuya Nakamura, Kohei Otomo

и другие.

Опубликована: Сен. 11, 2024

Plants distribute many nutrients to chloroplasts during leaf development and maturation. When leaves senesce or experience sugar starvation, the autophagy machinery degrades chloroplast proteins facilitate efficient nutrient reuse. Here, we report on intracellular dynamics of an pathway responsible for piecemeal degradation components. Through live-cell monitoring morphology, observed formation budding structures in sugar-starved leaves. These buds were then released incorporated into vacuolar lumen as autophagic cargo termed a Rubisco-containing body. The did not accumulate mutants core machinery, suggesting that autophagosome creation is required forming buds. Simultaneous tracking morphology revealed isolation membranes autophagosomes interact closely with part surface before Chloroplasts protrude at site associated membranes, which divide synchronously This autophagy-related division does require DYNAMIN-RELATED PROTEIN 5B, constitutes ring proliferation growing An unidentified may thus fragment coordination chloroplast-associated membrane.

Язык: Английский

Chloroplast Vesiculation and Induced Chloroplast Vesiculation and Senescence‐Associated Gene 12 Expression During Tomato Flower Pedicel Abscission DOI Creative Commons
Magda Tušek Žnidarič, Maja Zagorščak, Živa Ramšak

и другие.

Plant Direct, Год журнала: 2025, Номер 9(1)

Опубликована: Янв. 1, 2025

ABSTRACT Abscission is a tightly regulated process in which plants shed unnecessary, infected, damaged, or aging organs, as well ripe fruits, through predetermined abscission zones response to developmental, hormonal, and environmental signals. Despite its importance, the underlying mechanisms remain incompletely understood. This study highlights deleterious effects of on chloroplast ultrastructure cells tomato flower pedicel zone, revealing spatiotemporal differential gene expression key transcriptional networks involved vesiculation during abscission. Significant changes structure vesicle formation were observed 8 14 h after induction, coinciding with vesiculation‐related genes, particularly upregulation Senescence‐Associated Gene 12 ( SAG12 ) Chloroplast Vesiculation CV ). suggests possible transport degrading material for recycling by autophagy‐independent senescence‐associated vacuoles (SAVs) CV‐containing vesicles (CCVs). Ethylene signaling appears be regulation these processes, treatment competitive inhibitor ethylene action, 1‐methylcyclopropene, delayed vesiculation, reduced , increased Curvature Thylakoid 1A CURT1A In addition, was associated photosynthesis‐related those light reactions, underscoring functional impact structural changes. work provides new insights into molecular ultrastructural offers potential targets agricultural biotechnological applications.

Язык: Английский

Процитировано

2
A synthetic chlorophagy receptor promotes plant fitness by mediating chloroplast microautophagy DOI Creative Commons
Rui Liu, Xun Weng,

Xinjing Li

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Фев. 25, 2025

Abstract Chloroplasts are major photosynthetic and protein-containing organelles in green plants algae. Unwanted chloroplast proteins entire chloroplasts cleared through various degradation pathways including autophagy. Nevertheless, canonical chlorophagy receptors remain unidentified, whether to what extent can be enhanced benefect the unknown. Here we designed validated a synthetic autophagy receptor using biochemical, genetical, imaging approaches. This receptor, LIR-SNT-BFP, was constructed by fusing fragment containing LC3-interacting region (LIR) of selective NBR1 N-terminal amphipathic α-helix outer envelope protein SFR2. The fusion LIR-SNT-BFP coated attracted ATG8a planta . Upon induction, elicited vacuole-mediated microautophagy independent ATG8 conjugation machinery ATG5 or ATG7. Meanwhile, it induced division; however PDV2. Notably, moderate improves rosette growth, but excessive levels detrimental. Furthermore, partially protects against herbicide-induced leaf chlorosis. study demonstrates controlled receptor.

Язык: Английский

Процитировано

1
Vacuolar Degradation of Plant Organelles DOI
Marisa S. Otegui, Charlotte Steelheart, Wenlong Ma

и другие.

The Plant Cell, Год журнала: 2024, Номер 36(9), С. 3036 - 3056

Опубликована: Апрель 24, 2024

Plants continuously remodel and degrade their organelles due to damage from metabolic activities environmental stressors, as well an integral part of cell differentiation programs. Whereas certain use local hydrolytic enzymes for limited remodeling, most the pathways that control partial or complete dismantling rely on vacuolar degradation. Specifically, selective autophagic play a crucial role in recognizing sorting plant organelle cargo clearance, especially under cellular stress conditions induced by factors like heat, drought, damaging light. In these short reviews, we discuss mechanisms degradation chloroplasts, mitochondria, endoplasmic reticulum, Golgi, peroxisomes, with emphasis autophagy, recently discovered autophagy receptors organelles, crosstalk other catabolic pathways.

Язык: Английский

Процитировано

8
Ubiquitin-mediated degradation of SlPsbS regulates low night temperature tolerance in tomatoes DOI Creative Commons

Jiazhi Lu,

Junchi Yu,

Pengkun Liu

и другие.

Cell Reports, Год журнала: 2024, Номер 43(10), С. 114757 - 114757

Опубликована: Сен. 19, 2024

Язык: Английский

Процитировано

6
A synthetic chlorophagy receptor promotes plant fitness by mediating chloroplast microautophagy DOI Creative Commons
Rui Liu, Xun Weng,

Xinjing Li

и другие.

Cell Reports, Год журнала: 2025, Номер 44(6), С. 115759 - 115759

Опубликована: Май 30, 2025

Язык: Английский

Процитировано

0
Chloroplast Auxin Efflux Mediated by ABCB28 and ABCB29 Fine-Tunes Salt and Drought Stress Responses in Arabidopsis DOI Creative Commons

Prashanth Tamizhselvan,

Sharmila Madhavan,

Christian Constán‐Aguilar

и другие.

Plants, Год журнала: 2023, Номер 13(1), С. 7 - 7

Опубликована: Дек. 19, 2023

Photosynthesis is among the first processes negatively affected by environmental cues and its performance directly determines plant cell fitness ultimately crop yield. Primarily sites of photosynthesis, chloroplasts are unique also for biosynthesis precursors growth regulator auxin sensing stress, but their role in intracellular homeostasis, vital survival changing environments, remains poorly understood. Here, we identified two ATP-binding cassette (ABC) subfamily B transporters, ABCB28 ABCB29, which export across chloroplast envelope to cytosol a concerted action vivo. Moreover, provide evidence an pathway Arabidopsis thaliana chloroplasts. The overexpression ABCB29 influenced stomatal regulation resulted significantly improved water use efficiency rates during salt drought stresses. Our results suggest that production transport contribute stomata conserving upon stress. integrate photosynthesis signals as such hold great potential improve adaptation crops cues.

Язык: Английский

Процитировано

6
Identification and Expression Analysis of Soybean (Glycine max L.) Dynamin Genes Reveal Their Involvements in Plant Development and Stress Response DOI
Xiangbo Duan,

Yanang Xu,

Ke Zhang

и другие.

Tropical Plant Biology, Год журнала: 2024, Номер 17(3), С. 180 - 195

Опубликована: Май 4, 2024

Язык: Английский

Процитировано

2
Autophagosome development and chloroplast segmentation occur synchronously for piecemeal degradation of chloroplasts DOI Creative Commons
Masanori Izumi, Sakuya Nakamura, Kohei Otomo

и другие.

eLife, Год журнала: 2024, Номер 12

Опубликована: Ноя. 7, 2024

Plants distribute many nutrients to chloroplasts during leaf development and maturation. When leaves senesce or experience sugar starvation, the autophagy machinery degrades chloroplast proteins facilitate efficient nutrient reuse. Here, we report on intracellular dynamics of an pathway responsible for piecemeal degradation components. Through live-cell monitoring morphology, observed formation budding structures in sugar-starved leaves. These buds were then released incorporated into vacuolar lumen as autophagic cargo termed a Rubisco-containing body. The did not accumulate mutants core machinery, suggesting that autophagosome creation is required forming buds. Simultaneous tracking morphology revealed isolation membranes autophagosomes interact closely with part surface before Chloroplasts protrude at site associated membranes, which divide synchronously This autophagy-related division does require DYNAMIN-RELATED PROTEIN 5B, constitutes ring proliferation growing An unidentified may thus fragment coordination chloroplast-associated membrane.

Язык: Английский

Процитировано

1
Autophagosome development and chloroplast segmentation occur synchronously for piecemeal degradation of chloroplasts DOI Creative Commons
Masanori Izumi, Sakuya Nakamura, Kohei Otomo

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Окт. 14, 2023

Abstract Plants distribute many nutrients to chloroplasts during leaf development and maturation. When leaves senesce or experience sugar starvation, the autophagy machinery degrades chloroplast proteins facilitate efficient nutrient reuse. Here, we report on intracellular dynamics of an pathway responsible for piecemeal degradation components. Through live-cell monitoring morphology, observed formation budding structures in sugar-starved leaves. These buds were then released incorporated into vacuolar lumen as autophagic cargo termed a Rubisco-containing body. The did not accumulate mutants core machinery, suggesting that autophagosome creation is required forming buds. Simultaneous tracking morphology revealed isolation membranes autophagosomes interact closely with part surface before Chloroplasts protrude at site associated membranes, which divide synchronously This autophagy-related division does require DYNAMIN-RELATED PROTEIN 5B, constitutes ring proliferation growing An unidentified may thus fragment coordination chloroplast-associated membrane.

Язык: Английский

Процитировано

1
Autophagosome development and chloroplast segmentation occur synchronously for piecemeal degradation of chloroplasts DOI Creative Commons
Masanori Izumi, Sakuya Nakamura, Kohei Otomo

и другие.

eLife, Год журнала: 2023, Номер 12

Опубликована: Дек. 19, 2023

Plants distribute many nutrients to chloroplasts during leaf development and maturation. When leaves senesce or experience sugar starvation, the autophagy machinery degrades chloroplast proteins facilitate efficient nutrient reuse. Here, we report on intracellular dynamics of an pathway responsible for piecemeal degradation components. Through live-cell monitoring morphology, observed formation budding structures in sugar-starved leaves. These buds were then released incorporated into vacuolar lumen as autophagic cargo termed a Rubisco-containing body. The did not accumulate mutants core machinery, suggesting that autophagosome creation is required forming buds. Simultaneous tracking morphology revealed isolation membranes autophagosomes interact closely with part surface before Chloroplasts protrude at site associated membranes, which divide synchronously This autophagy-related division does require DYNAMIN-RELATED PROTEIN 5B, constitutes ring proliferation growing An unidentified may thus fragment coordination chloroplast-associated membrane.

Язык: Английский

Процитировано

1