An optogenetic toolkit for robust activation of FGF, BMP, & Nodal signaling in zebrafish
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 19, 2025
ABSTRACT
Cell
signaling
regulates
a
wide
range
of
biological
processes
including
development,
homeostasis,
and
disease.
Accessible
technologies
to
precisely
manipulate
have
important
applications
in
basic
translational
research.
Here,
we
introduce
an
optogenetic
toolkit
comprised
1)
zebrafish-optimized
FGF
activator,
2)
single-transcript
Nodal
3)
previously
established
BMP
activator.
We
thoroughly
characterize
this
suite
tools
zebrafish
embryos
show
that
they
provide
tunable,
light-dependent
spatiotemporal
control
vivo
.
In
response
blue
light
(∼455
nm),
receptor
kinase
domains
fused
light-dimerizing
LOV
enable
robust
activation
with
minimal
ectopic
activity
the
dark
or
at
wavelengths
over
495
nm.
Optogenetic
by
each
tool
is
pathway-specific
results
increased
expression
known
target
genes.
Signaling
activated
rapid
on/off
kinetics,
strength
depends
on
irradiance.
Finally,
demonstrate
spatially
localized
our
optimized
Together,
establish
as
potent
experimental
platform
rapidly,
directly,
adjustably
activate
FGF,
BMP,
embryos.
Язык: Английский
Healing beyond tradition: Advanced regenerative therapies for joint-related sports injuries
The Innovation Medicine,
Год журнала:
2025,
Номер
unknown, С. 100132 - 100132
Опубликована: Янв. 1, 2025
<p>Sports
injuries
are
one
of
the
most
common
diseases,
and
repair
regeneration
joint
soft
tissues
(ligaments,
tendons,
cartilage,
etc.)
involved
in
them
challenging.
Traditional
treatment
strategies,
such
as
drugs,
physical
therapy,
surgery,
difficult
to
achieve
tissue
reconstruction
natural
physiological
functions.
Regenerative
medicine
offers
promising
solutions,
including
biotherapy,
engineering,
prosthesis
or
organ
transplantation.
These
approaches
aim
regenerate
damaged
tissues,
reduce
recovery
time,
improve
functional
outcomes.
Stem
cells
from
different
sources
their
constructed
organoids,
novel
biomaterials,
3D
printing
other
innovative
technologies
have
been
used.
Individual
variability,
uncertain
long-term
efficacy,
high
costs
remain
obstacles
for
clinical
application.
Further
research
is
needed
explore
molecular
mechanisms
underlying
regenerative
therapies
orchestrate
biological,
chemical
factors.</p>
Язык: Английский
Transforming growth factor beta signaling and craniofacial development: modeling human diseases in zebrafish
Frontiers in Cell and Developmental Biology,
Год журнала:
2024,
Номер
12
Опубликована: Фев. 7, 2024
Humans
and
other
jawed
vertebrates
rely
heavily
on
their
craniofacial
skeleton
for
eating,
breathing,
communicating.
As
such,
it
is
vital
that
the
elements
of
develop
properly
during
embryogenesis
to
ensure
a
high
quality
life
evolutionary
fitness.
Indeed,
abnormalities,
including
cleft
palate
craniosynostosis,
represent
some
most
common
congenital
abnormalities
in
newborns.
Like
many
organ
systems,
development
complex,
relying
specification
migration
neural
crest,
patterning
pharyngeal
arches,
morphogenesis
each
skeletal
element
into
its
final
form.
These
processes
must
be
carefully
coordinated
integrated.
One
way
this
achieved
through
spatial
temporal
deployment
cell
signaling
pathways.
Recent
studies
conducted
using
zebrafish
model
underscore
importance
Transforming
Growth
Factor
Beta
(TGF-β)
Bone
Morphogenetic
Protein
(BMP)
pathways
development.
Although
both
contain
similar
components,
pathway
results
unique
outcomes
cellular
level.
In
review,
we
will
cover
show
necessity
these
stage
development,
starting
with
induction
ending
elements.
We
also
human
diseases
malformations
caused
by
mutations
components
(e.g.,
palate,
etc.)
potential
utility
studying
etiology
diseases.
briefly
joint
biology
discuss
role
TGF-β/BMP
result
from
aberrancies
pathways,
osteoarthritis
multiple
synostoses
syndrome.
Overall,
review
demonstrate
critical
roles
disease.
Язык: Английский