Cell-class-specific orofacial motor maps in mouse neocortex

Current Biology, Год журнала: 2025, Номер unknown

Опубликована: Фев. 1, 2025

Cortical motor maps represent fundamental organizing principles for voluntary control,1 yet their underlying structure remains poorly understood, including regions of sensory2,3 and parietal cortex,4 as well the classical frontal cortex. To understand how anatomically distinct cortical areas are organized into functional units controlling movements, here, we refined by selectively stimulating genetically defined subpopulations excitatory neurons. Surprisingly, found spatially segregated modules in orofacial optogenetically different classes The overall map jaw opening revealed all neurons spanned anterior lateral cortex broadly. By contrast, jaw-opening specific cell were focalized either primary motor, secondary or somatosensory within neurons, demonstrating cell-class-specific modules. Simultaneous wide-field calcium imaging activity propagation from optically stimulated to area correlating with movement vigor. largely stable across lick learning important exceptions indicating expansion other module zones. Our data suggest that interacting sensorimotor cortices might contribute movement.

Язык: Английский

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Stimulus selection enhances value-modulated somatosensory processing in the superior colliculus

PLoS Biology, Год журнала: 2025, Номер 23(3), С. e3003057 - e3003057

Опубликована: Март 31, 2025

A fundamental trait of intelligent behavior is the ability to respond selectively stimuli with higher value. Where along neural hierarchy does somatosensory processing transition from a map stimulus location value? To address this question, we recorded single-unit activity populations neurons in cortex (S1) and midbrain superior colliculus (SC) mice conditioned positive-valued withhold responses an adjacent, negative-valued stimulus. The preference S1 population was equally weighted towards either stimulus, line somatotopic map. Surprisingly, discovered large SC that were disproportionately biased positive This disproportionate bias largely driven by enhanced spike suppression for negative Removing opportunity behaviorally select reduced spontaneous firing rates but not S1, suggesting selectivity augmented task readiness. Similarly, predicted reaction times, played persistent role perceptual decision-making. Taken together, these data indicate transformed into value-based encodes priority.

Язык: Английский

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Cell class-specific long-range axonal projections of neurons in mouse whisker-related somatosensory cortices

eLife, Год журнала: 2024, Номер 13

Опубликована: Май 17, 2024

Long-range axonal projections of diverse classes neocortical excitatory neurons likely contribute to brain-wide interactions processing sensory, cognitive and motor signals. Here, we performed light-sheet imaging fluorescently labeled axons from genetically defined located in posterior primary somatosensory barrel cortex supplemental cortex. We used convolutional networks segment axon-containing voxels quantified their distribution within the Allen Mouse Brain Atlas Common Coordinate Framework. Axonal density was analyzed for different glutamatergic using transgenic mouse lines selectively expressing Cre recombinase layer 2/3 intratelencephalic projection (Rasgrf2-dCre), 4 (Scnn1a-Cre), 5 (Tlx3-Cre), pyramidal tract (Sim1-Cre), (Rbp4-Cre), 6 corticothalamic (Ntsr1-Cre). found distinct neuronal many downstream brain areas, which were largely similar supplementary cortices. Functional connectivity maps obtained optogenetic activation sensory wide-field revealed topographically organized evoked activity frontal with more laterally signaling anteriorly regions cortex, consistent anatomical projections. The current methodology therefore appears quantify innervation patterns supporting signaling.

Язык: Английский

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Cell class-specific long-range axonal projections of neurons in mouse whisker-related somatosensory cortices

eLife, Год журнала: 2024, Номер 13

Опубликована: Окт. 11, 2024

Long-range axonal projections of diverse classes neocortical excitatory neurons likely contribute to brain-wide interactions processing sensory, cognitive and motor signals. Here, we performed light-sheet imaging fluorescently labeled axons from genetically defined located in posterior primary somatosensory barrel cortex supplemental cortex. We used convolutional networks segment axon-containing voxels quantified their distribution within the Allen Mouse Brain Atlas Common Coordinate Framework. Axonal density was analyzed for different glutamatergic using transgenic mouse lines selectively expressing Cre recombinase layer 2/3 intratelencephalic projection (Rasgrf2-dCre), 4 (Scnn1a-Cre), 5 (Tlx3-Cre), pyramidal tract (Sim1-Cre), (Rbp4-Cre), 6 corticothalamic (Ntsr1-Cre). found distinct neuronal many downstream brain areas, which were largely similar supplementary cortices. Functional connectivity maps obtained optogenetic activation sensory wide-field revealed topographically organized evoked activity frontal with more laterally signaling anteriorly regions cortex, consistent anatomical projections. The current methodology therefore appears quantify innervation patterns supporting signaling.

Язык: Английский

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Environmental context influences visual processing in thalamus

Current Biology, Год журнала: 2025, Номер unknown

Опубликована: Март 1, 2025

Язык: Английский

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Sparse innervation and local heterogeneity in the vibrissal corticostriatal projection

Опубликована: Май 7, 2025

The density and overlap of cortical axons in the dorsolateral striatum (DLS) have suggested that striatal neurons integrate widespread information from regions are functionally related. However, vivo, DLS neuronal responses to sensory stimuli shown unexpectedly high selectivity, raising questions about actual degree input convergence functional corticostriatal projection on individual cells. Here, we investigated this question by focusing projections different whisker columns, as they co-active during behavior. Using ex vivo patch-clamp recordings glutamate uncaging for focal stimulations barrel cortex, were able map location presynaptic (SPNs). We found each SPN was innervated cells located a small number columns scattered across field slice. Connectivity single SPNs with thus highly discontinuous horizontally, despite presence more potential connections. Moreover, connectivity patterns specific cell, neighboring sharing few common clusters cortex. Despite sparse distinct innervation SPNs, topographically organized at population level. Finally, similar D1 D2-type but observed differences synaptic strength connections layers, notably associative layer 2/3. Our results suggest somatosensory inputs striatum, enabled diffuse overlapping innervation, is accomplished through yet complementary SPNs.

Язык: Английский

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Sparse innervation and local heterogeneity in the vibrissal corticostriatal projection

Опубликована: Май 7, 2025

The density and overlap of cortical axons in the dorsolateral striatum (DLS) have suggested that striatal neurons integrate widespread information from regions are functionally related. However, vivo, DLS neuronal responses to sensory stimuli shown unexpectedly high selectivity, raising questions about actual degree input convergence functional corticostriatal projection on individual cells. Here, we investigated this question by focusing projections different whisker columns, as they co-active during behavior. Using ex vivo patch-clamp recordings glutamate uncaging for focal stimulations barrel cortex, were able map location presynaptic (SPNs). We found each SPN was innervated cells located a small number columns scattered across field slice. Connectivity single SPNs with thus highly discontinuous horizontally, despite presence more potential connections. Moreover, connectivity patterns specific cell, neighboring sharing few common clusters cortex. Despite sparse distinct innervation SPNs, topographically organized at population level. Finally, similar D1 D2-type but observed differences synaptic strength connections layers, notably associative layer 2/3. Our results suggest somatosensory inputs striatum, enabled diffuse overlapping innervation, is accomplished through yet complementary SPNs.

Язык: Английский

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Cell class-specific long-range axonal projections of neurons in mouse whisker-related somatosensory cortices

Опубликована: Май 17, 2024

The extensive long-range axonal projections of various classes neocortical excitatory neurons are thought to contribute importantly the highly integrative brain-wide interactions underlying processing sensory, cognitive and motor signals. Here, we investigated output genetically-defined projection with cell bodies located in whisker-related somatosensory cortices mouse through light-sheet imaging fluorescently-labeled axons segmented by specifically-trained convolutional networks quantified within Allen Mouse Brain Atlas Common Coordinate Framework. We injected Cre-dependent virus express GFP or tdTomato posterior primary barrel cortex supplemental cortex, which contain representations large mystacial whiskers. six following transgenic lines: Rasgrf2-dCre, Scnn1a-Cre, Tlx3-Cre, Sim1-Cre, Rbp4-Cre Ntsr1-Cre. found many diverse downstream brain areas showing distinct innervation patterns. To test whether revealed might underpin functional circuits, compared spatial organization connectivity maps obtained from optogenetic stimulation sensory wide-field activity propagation frontal cortices. Both methods indicated that more laterally topographically signaled anteriorly regions cortex. current methodology therefore appears quantify patterns supporting signaling, and, together further technological advances, this will help provide increasingly detailed information brain, essential for understanding complex neuronal circuitry even simple goal-directed behaviors.

Язык: Английский

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Cell class-specific long-range axonal projections of neurons in mouse whisker-related somatosensory cortices

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Март 22, 2024

Abstract The extensive long-range axonal projections of diverse classes neocortical excitatory neurons are thought to contribute importantly the highly integrative brain-wide interactions underlying processing sensory, cognitive and motor signals. Here, we investigated output various genetically-defined projection with cell bodies located in whisker-related somatosensory cortices mouse through light-sheet imaging fluorescently-labeled axons segmented by specifically-trained convolutional networks quantified within Allen Mouse Brain Atlas Common Coordinate Framework. We injected Cre-dependent virus express GFP or tdTomato posterior primary barrel cortex supplemental cortex, which contain representations large mystacial whiskers. six following transgenic lines preferentially Cre different glutamatergic classes: Rasgrf2-dCre for layer 2/3 intratelencephalic neurons, Scnn1a-Cre 4 Tlx3-Cre 5 Sim1-Cre pyramidal tract Rbp4-Cre Ntsr1-Cre 6 corticothalamic neurons. found many downstream brain areas largely similar from secondary cortices, but showing distinct innervation patterns, mice broadest targets, subsets were innervated other lines. To test whether revealed might underpin functional circuits, compared spatial organization connectivity maps obtained optogenetic stimulation sensory wide-field activity propagation frontal cortices. Both methods indicated that more laterally topographically signaled anteriorly regions cortex. current methodology therefore appears quantify patterns supporting signaling, and, together further technological advances, this will help provide increasingly detailed information brain, essential understanding complex neuronal circuitry even simple goal-directed behaviors.

Язык: Английский

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Stimulus selection drives value-modulated somatosensory processing in superior colliculus

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Июль 1, 2024

Abstract A fundamental trait of intelligent behavior is the ability to respond selectively stimuli with higher value. Where along somatosensory hierarchy does information transition from a map stimulus location value? To address this question, we recorded single-unit activity populations neurons in cortex (S1) and midbrain superior colliculus (SC) mice conditioned positive-valued whisker withhold responses using an adjacent, negative-valued stimulus. The preference S1 population was equally weighted towards either whisker, line somatotopic map. Surprisingly, discovered large SC that were disproportionately biased positive This disproportionate bias controlled by spike facilitation for suppression negative single neurons. Removing opportunity select reduced but not S1, suggesting sensory processing partially movement preparation. Similarly, spontaneous firing rates accurately predicted reaction times, play persistent role perceptual decision-making. Taken together, these data indicate transformed into value-based encodes priority.

Язык: Английский

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