Neuromodulator and neuropeptide sensors and probes for precise circuit interrogation in vivo
Science,
Год журнала:
2024,
Номер
385(6716)
Опубликована: Сен. 26, 2024
To
determine
how
neuronal
circuits
encode
and
drive
behavior,
it
is
often
necessary
to
measure
manipulate
different
aspects
of
neurochemical
signaling
in
awake
animals.
Optogenetics
calcium
sensors
have
paved
the
way
for
these
types
studies,
allowing
perturbation
readout
spiking
activity
within
genetically
defined
cell
types.
However,
methods
lack
ability
further
disentangle
roles
individual
neuromodulator
neuropeptides
on
behavior.
We
review
recent
advances
chemical
biology
tools
that
enable
precise
spatiotemporal
monitoring
control
over
neuroeffectors
their
receptors
vivo.
also
highlight
discoveries
enabled
by
such
tools,
revealing
molecules
signal
across
timescales
learning,
orchestrate
behavioral
changes,
modulate
circuit
activity.
Язык: Английский
Mu Opioid Agonistic Effect on Neuropeptide Gene Expression Levels Involved in Hypothalamic Feeding Regulation
Archives of Current Medical Research,
Год журнала:
2025,
Номер
6(1), С. 1 - 7
Опубликована: Янв. 30, 2025
Background:
The
regulation
of
food
intake
in
the
hypothalamus
is
one
most
complicated
through
integration
various
neuroendocrine
mechanisms.
In
this
region,
orexigenic
and
anorexigenic
peptides
play
a
role
by
responding
to
different
stimuli.
Additionally,
central
opioidergic
systems
are
involved
feeding
behavior.
Several
neuropeptides
expressed
also
contribute
intake.
aim
study
was
investigate
effects
mu
agonist/antagonist
molecules
on
both
gene
expression
levels
hypothalamus.
Methods:
our
study,
48
male
Wistar
Albino
rats
were
divided
into
4
groups
as
control,
morphine,
naloxone
morphine+naloxone.
control
group
received
subcutaneous
SF
solution
for
5
days;
morphine
at
dose
10
mg/kg/day
administered
days
3
mg/kg
1.5
hours
after
last
injection:
morphine+naloxone
injection.
Hypothalamus
tissues
isolated
from
brains
end
experimental
period.
Anorexigenic
peptide
analysed
RT-PCR
method.
Differences
between
statistically
analyzed
using
one-way
factorial
ANOVA
Tukey
post-hoc
test.
Results:
Morphine
administration
results
decrease
OX2R
LepR
genes,
but
did
not
change
ORXA,
OX1R,
AgRP,
NPY,
POMC
expression.
Naloxone
increased
AgRP
NPY
while
decreasing
OX2R,
APLNR
levels.
Conclusions:
Our
findings
suggest
that
may
affect
related
nutrition
metabolism
Further
studies
needed
clarify
possible
mechanistic
opiodergic
activity
dependence
manner.
Язык: Английский
Cannabinoids Drive Feeding Through Agrp Neurons
Опубликована: Янв. 1, 2025
Язык: Английский
Endogenous opioid receptors and the feast or famine of maladaptive feeding
Nature Communications,
Год журнала:
2025,
Номер
16(1)
Опубликована: Март 6, 2025
Maladaptive
feeding
comprises
unhealthy
eating
patterns
that
jeopardize
survival,
including
over-
and
underconsumption.
These
behaviors
are
often
coordinated
by
endogenous
opioid
receptors
(EORs).
Here,
we
explore
the
involvement
of
EORs
in
obesity
anorexia
nervosa
(AN),
two
disorders
associated
with
dysregulated
behavior
relevant
animal
models.
While
seemingly
opposing
metabo-psychiatric
states,
our
goal
is
to
highlight
common
circuit
synaptic
mechanisms
underlying
AN
a
focus
on
EOR
functionality.
We
examine
neural
substrates
maladaptive
comorbid
conditions
pain,
suggesting
role
for
EOR-driven
plasticity
pathogenesis
both
AN.
Язык: Английский