Опубликована: Янв. 1, 2024
Язык: Английский
bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown
Опубликована: Авг. 28, 2024
SUMMARY The medial septum and diagonal band of Broca (MSDB) is known for its diverse populations cholinergic, GABAergic, glutamatergic neurons, each contributing to various cognitive processes. However, cell-specific manipulations within this region often result in incongruous behavioral outcomes reach conflicting conclusions, likely because a hitherto unknown molecular complexity cellular landscape. In study, we employed single-cell RNA sequencing thoroughly describe the heterogeneity MSDB neurons. We confirmed previously established neuronal classes, found gene expression gradients them revealed genetically defined subclusters. Moreover, characterized genetic profiles these subclusters mapped their spatial distribution. Our analysis presents comprehensive description provides marker genes target them, explains previous paradoxical results, opens unexplored avenues study impact neuromodulators basal forebrain.
Язык: Английский
Процитировано
0
bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown
Опубликована: Сен. 5, 2024
The mediodorsal thalamus (MD) and adjacent midline nuclei are important for cognition mental illness, but their cellular composition is not well defined. Using single-nucleus spatial transcriptomics, we identified a conserved excitatory neuron gradient, with distinct mapping of individual clusters. One end the gradient was expanded in human MD compared to mice, which may be related expansion granular prefrontal cortex hominids. Moreover, neurons preferentially onto parvocellular division were associated genetic risk schizophrenia bipolar disorder. Midbrain-derived inhibitory interneurons enriched implicated major depressive
Язык: Английский
Процитировано
0
Research Square (Research Square), Год журнала: 2024, Номер unknown
Опубликована: Окт. 25, 2024
Spatial transcriptomics has allowed researchers to analyze transcriptome data in its tissue sample's spatial context. Various methods have been developed for detecting spatially variable genes (SV genes), whose gene expression over the space shows strong autocorrelation. Such are often used define clusters cells or spots downstream. However, highly (HV) genes, quantitative expressions show significant variation from cell cell, conventionally clustering analyses. In this report, we investigate whether adding can improve type performance data. We tested of HV SV and union both sets (concatenation) on 50 real datasets across multiple platforms, using a variety non-spatial metrics. Our results that combining overall cell-type performance.
Язык: Английский
Процитировано
0
bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown
Опубликована: Ноя. 8, 2024
Summary Disorientation is an early symptom of dementia, suggesting impairments in neural circuits responsible for head direction signaling. The anterodorsal thalamic nucleus (ADn) exhibits and selective vulnerability to pathological misfolded forms tau (ptau), a major hallmark Alzheimer’s disease ageing. ADn contains high density (HD) cells; their disruption may contribute spatial disorientation. To test this, we virally expressed human the adult mice. HD-ptau mice were defined by ptau+ cells axon terminals postsynaptic target regions. Despite being able learn memory tasks, exhibited increased looping behavior during learning made greater number turns recall, consistent with Using vivo extracellular recordings, identified ptau-expressing found that from had reduced directionality altered burst firing. These data suggest ptau alters HD signaling, leading orientation.
Язык: Английский
Процитировано
0
Опубликована: Янв. 1, 2024
Язык: Английский
Процитировано
0