CHANGES IN THE STRUCTURE OF THE COLON WALL TISSUES UNDERTHE INFLUENCE OF BIOLOGICALLY ACTIVE SUBSTANCES DOI Creative Commons
P. V. Kirichek, H. V. Lukyantseva

Bulletin of Taras Shevchenko National University of Kyiv Series Biology, Год журнала: 2024, Номер 99(4), С. 27 - 33

Опубликована: Янв. 1, 2024

Background. The regulation of the motor function colon is carried out due to complex combined influence a endogenous nervous and humoral factors, which serve as modulators contractile activity muscles intestinal wall. However, issue use safety exogenous correctors remains extremely relevant in demand. Thus, search for potential factors capable physiologically modulating intestines kind challenge modern scientists, study features these substances on tissues wall scientific practical problem biological science. aim work was establish morphological changes structures under biologically active substances. Methods. conducted 94 sexually mature outbred male rats. effect histological structure sigmoid following studied: quercetin, caffeine, floccalin, phoridone, mixture floccalin E510. Rat preparations were incubated solutions 30 minutes vitro, after examination performed. Intestinal fragments fixed 10% neutral formalin, passed through ascending concentrations ethyl alcohol, then embedded paraffin. Sections 5-7 μm thick made from obtained paraffin samples, stained with hematoxylin eosin. Morphological assessed using microscope. Results. absence pathological their mixture, also quercetin caffeine recorded. This suggests that do not have harmful side effects relation histostructures E510 leads appearance signs lymphocytic infiltration, modification lymphoid follicles, loosening swelling submucosal base impaired capillary barrier function. Conclusions. action all studied substances, exception E510, causes minor structural or functional wall, are reactive reversible nature lead development irreversible phenomena. In contrast, adverse morphofunctional requires further in-depth study.

Язык: Английский

Gut dysbiosis was inevitable, but tolerance was not: temporal responses of the murine microbiota that maintain its capacity for butyrate production correlate with sustained antinociception to chronic morphine DOI Creative Commons

Izabella Sall,

Randi Foxall,

Lindsey Felth

и другие.

Gut Microbes, Год журнала: 2025, Номер 17(1)

Опубликована: Янв. 12, 2025

The therapeutic benefits of opioids are compromised by the development analgesic tolerance, which necessitates higher dosing for pain management thereby increasing liability drug dependence and addiction. Rodent models indicate opposing roles gut microbiota in tolerance: morphine-induced dysbiosis exacerbates whereas probiotics ameliorate tolerance. Not all individuals develop could be influenced differences microbiota, yet no study design has capitalized upon this natural variation. We leveraged behavioral variation a murine model voluntary oral morphine self-administration to elucidate mechanisms influences Although mice shared similar morphine-driven changes that largely masked informative associations with variability our high-resolution temporal analyses revealed divergence progression best explained sustained antinociception. Mice did not tolerance maintained capacity production short-chain fatty acid (SCFA) butyrate known bolster intestinal barriers promote neuronal homeostasis. Both fecal microbial transplantation (FMT) from donor dietary supplementation significantly reduced independently suppression systemic inflammation. These findings inform immediate therapies extend efficacy opioids.

Язык: Английский

Процитировано

0
Self-Organizing Assembloids Reveal Enteric Nervous System Dynamics in Gut Homeostasis and Regeneration DOI Creative Commons

Ilke Sari,

Baki Uzun,

Melda O. Oguz

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Янв. 14, 2025

The enteric nervous system (ENS) is essential for intestinal health, exhibiting adaptability to environmental and physiological challenges. However, the mechanisms underlying ENS plasticity resilience remain poorly understood. Organoid technology has revolutionized in vitro modeling by accurately replicating epithelial structures enabling significant advancements understanding gastrointestinal biology. traditional organoids are limited their ability study ENS, as they lack multicellular composition functional architecture necessary model complex interactions between neurons, glia, mesenchymal, smooth muscle, cells. To address these limitations, we developed murine ENS-Rich Assembloids (ERAs) that self-organize replicate cellular diversity, including structure, of native colonic tissue. These assembloids recreate neuron-glia interactions, reflect regenerative processes, provide a novel platform studying dynamics under controlled conditions. Integrating findings from an vivo model, demonstrate inflammation induces coordinated reorganization S100b+ glial cells, TUJ1+ PDGFRA+ mesenchymal revealing conserved plasticity. We identify pleiotrophin (PTN) signaling via Protein Tyrosine Phosphatase Receptor Type Z1 (PTPRZ1) key pathway facilitating neural elongation enhancing interactions. Moreover, show activated neurons transfer lipids support mechanism during inflammation. position glia protective hubs fostering tissue regeneration. By building on foundational success organoid addressing its limitations ENS-rich establish transformative tool investigating responses disease, repair.

Язык: Английский

Процитировано

0
mpactR: an R adaptation of the metabolomics peak analysis computational tool (MPACT) for use in reproducible data analysis pipelines DOI Open Access
Allison R. Mason, Gregory Johnson,

Joseph Krampen

и другие.

Microbiology Resource Announcements, Год журнала: 2025, Номер unknown

Опубликована: Янв. 15, 2025

mpactR automates pre-processing of liquid chromatography-tandem mass spectrometry (LC-MS/MS) data from microbiological samples to correct mispicked peaks, resolve inter-sample variation in abundance across technical replicates, account for in-source ion fragmentation, and remove background noise yield high-quality features. The package is available through CRAN GitHub.

Язык: Английский

Процитировано

0
Fecal microbiota and metabolite composition associates with stool consistency in young children DOI Open Access
Anna‐Katariina Aatsinki, Heidi Isokääntä, Matilda Kråkström

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Июнь 9, 2024

ABSTRACT Stool consistency explains a large proportion of inter-individual variation in gut microbiota and represents useful measure transit time, which is pivotal for bacterial metabolism vice versa . However, infants small children, detailed understanding how the metabolome associate with stool lacking. We analyzed both 2.5 (n=360), 6 (n=229), 14 (n=274), 30-month-old children (n=169) from FinnBrain Birth Cohort Study using 16s rRNA sequencing mass spectrometry-based targeted techniques. used water content parent-reported as proxies consistency. found that community composition well relative abundance specific genera such Clostridium , Hungatella Lactobacillus were associated Of metabolites, lower concentrations conjugated bile acids acetic acid having constipation content. Branched short-chain fatty negatively The findings reflect shift saccharolytic to proteolytic gut. Parental crude reports agreed objective child consistency, similar features metabolome. Overall, our support concept total metabolic activity young children.

Язык: Английский

Процитировано

1
T cells regulate intestinal motility and shape enteric neuronal responses to intestinal microbiota DOI Creative Commons
Patricia Souza,

Catherine M. Keenan,

Laurie E. Wallace

и другие.

Gut Microbes, Год журнала: 2024, Номер 17(1)

Опубликована: Дек. 20, 2024

How the gut microbiota and immune system maintain intestinal homeostasis in concert with enteric nervous (ENS) remains incompletely understood. To address this gap, we assessed small transit, neuronal density, neurogenesis, microbiota, cell populations cytokines wildtype T-cell deficient germ-free mice colonized specific pathogen-free (SPF) conventionally raised SPF segmented filamentous bacteria (SFB)-monocolonized mice. increased transit a T cell-dependent manner. density myenteric submucosal plexuses of ileum colon, similar to mice, independently cells. SFB manner, but cells colon. stimulated neurogenesis (Sox2 expression neurons) colon effect was cell-independent. regulated nestin ENS. colonization Th17 cells, RORγT+ Treg IL-1β IL-17A levels By neutralizing IL-17A, observed that they control microbiota-mediated were not involved regulation motility. Together, these findings provide new insights into microbiota-neuroimmune dialog regulates physiology.

Язык: Английский

Процитировано

1
Gut dysbiosis was inevitable, but tolerance was not: temporal responses of the murine microbiota that maintain its capacity for butyrate production correlate with sustained antinociception to chronic voluntary morphine DOI Creative Commons

Izabella Sall,

Randi Foxall,

Lindsey Felth

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Апрель 17, 2024

The therapeutic benefits of opioids are compromised by the development analgesic tolerance, which necessitates higher dosing for pain management thereby increasing liability dependence and addiction. Rodent models indicate opposing roles gut microbiota in tolerance: morphine-induced dysbiosis exacerbates whereas probiotics ameliorate tolerance. Not all individuals develop tolerance could be influenced differences microbiota, yet no study has capitalized upon this natural variation to identify specific features linked We leveraged a murine model voluntary oral morphine self-administration elucidate mechanisms influences Although mice shared similar predictive morphine-driven changes that largely masked informative associations with variability our high-resolution temporal analyses revealed divergence progression best explained Mice did not also maintained abundance taxa capable producing short-chain fatty acid (SCFA) butyrate, known bolster intestinal barriers, suppress inflammation, promote neuronal homeostasis. Furthermore, dietary butyrate supplementation significantly reduced These findings inform immediate therapies extend efficacy opioids.

Язык: Английский

Процитировано

0
T cells regulate intestinal motility and shape enteric neuronal responses to intestinal microbiota. DOI Open Access
Patricia Souza,

Catherine M. Keenan,

Laurie E. Wallace

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Май 24, 2024

Abstract The gut microbiota and immune system maintain intestinal homeostasis regulate physiology in concert with the enteric nervous (ENS). However, underlying mechanisms remain incompletely understood. Using wildtype T-cell deficient germ-free mice colonized segmented filamentous bacteria (SFB) or specific pathogen-free (SPF) microbiota, we studied regulation of ENS motility. Colonization markedly increased Th17 cells Treg expressing RORγ + T both ileum colon mice. were necessary for normalization motility after colonization by SPF SFB to restore neuronal density also required neurogenic responses myenteric neurons ileum, but not colon, regulating levels nestin expression. cytokines IL-1β IL-17A mediate response an involved Together, our findings provide new insights into microbiota-neuroimmune dialogue that regulates physiology.

Язык: Английский

Процитировано

0
CHANGES IN THE STRUCTURE OF THE COLON WALL TISSUES UNDERTHE INFLUENCE OF BIOLOGICALLY ACTIVE SUBSTANCES DOI Creative Commons
P. V. Kirichek, H. V. Lukyantseva

Bulletin of Taras Shevchenko National University of Kyiv Series Biology, Год журнала: 2024, Номер 99(4), С. 27 - 33

Опубликована: Янв. 1, 2024

Background. The regulation of the motor function colon is carried out due to complex combined influence a endogenous nervous and humoral factors, which serve as modulators contractile activity muscles intestinal wall. However, issue use safety exogenous correctors remains extremely relevant in demand. Thus, search for potential factors capable physiologically modulating intestines kind challenge modern scientists, study features these substances on tissues wall scientific practical problem biological science. aim work was establish morphological changes structures under biologically active substances. Methods. conducted 94 sexually mature outbred male rats. effect histological structure sigmoid following studied: quercetin, caffeine, floccalin, phoridone, mixture floccalin E510. Rat preparations were incubated solutions 30 minutes vitro, after examination performed. Intestinal fragments fixed 10% neutral formalin, passed through ascending concentrations ethyl alcohol, then embedded paraffin. Sections 5-7 μm thick made from obtained paraffin samples, stained with hematoxylin eosin. Morphological assessed using microscope. Results. absence pathological their mixture, also quercetin caffeine recorded. This suggests that do not have harmful side effects relation histostructures E510 leads appearance signs lymphocytic infiltration, modification lymphoid follicles, loosening swelling submucosal base impaired capillary barrier function. Conclusions. action all studied substances, exception E510, causes minor structural or functional wall, are reactive reversible nature lead development irreversible phenomena. In contrast, adverse morphofunctional requires further in-depth study.

Язык: Английский

Процитировано

0