Massively parallel mutant selection identifies genetic determinants ofPseudomonas aeruginosacolonization ofDrosophila melanogaster DOI Creative Commons
Jessica Miles, Gabriel L. Lozano, Jeyaprakash Rajendhran

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Ноя. 21, 2023

Abstract Pseudomonas aeruginosa is recognized for its ability to colonize diverse habitats and cause disease in a variety of hosts, including plants, invertebrates, mammals. Understanding how this bacterium able occupy wide-ranging niches important deciphering ecology. We used transposon sequencing (Tn-Seq, also known as INSeq) identify genes P. that contribute fitness during colonization Drosophila melanogaster . Our results reveal suite critical factors, those polysaccharide production, DNA repair, metabolism, respiration. Comparison candidate with determinants discovered previous studies identified several required virulence are conserved across hosts tissues. This analysis provides evidence both the conservation function systems, well host-specific functions. These findings, which represent first use gut pathogen , demonstrate power Tn-Seq fly model system advance existing knowledge intestinal pathogenesis by D. melanogaster, revealing bacterial comprehensive portrait P lifestyles habitats. Importance powerful understanding host-pathogen interactions. Research has yielded notable insights into mechanisms host immunity defense, many emerged from mutants defective well-characterized factors. foundational – advances high-throughput support unbiased screens fly. To investigate interplay exploit tractability host, we high-throughput, genome-wide mutant find enable pathogen, reveals mediators establishment some mouse systems. findings utility massively parallel provide platform aligning toolkit genomics.

Язык: Английский

Massively parallel mutant selection identifies genetic determinants ofPseudomonas aeruginosacolonization ofDrosophila melanogaster DOI Creative Commons
Jessica Miles, Gabriel L. Lozano, Jeyaprakash Rajendhran

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Ноя. 21, 2023

Abstract Pseudomonas aeruginosa is recognized for its ability to colonize diverse habitats and cause disease in a variety of hosts, including plants, invertebrates, mammals. Understanding how this bacterium able occupy wide-ranging niches important deciphering ecology. We used transposon sequencing (Tn-Seq, also known as INSeq) identify genes P. that contribute fitness during colonization Drosophila melanogaster . Our results reveal suite critical factors, those polysaccharide production, DNA repair, metabolism, respiration. Comparison candidate with determinants discovered previous studies identified several required virulence are conserved across hosts tissues. This analysis provides evidence both the conservation function systems, well host-specific functions. These findings, which represent first use gut pathogen , demonstrate power Tn-Seq fly model system advance existing knowledge intestinal pathogenesis by D. melanogaster, revealing bacterial comprehensive portrait P lifestyles habitats. Importance powerful understanding host-pathogen interactions. Research has yielded notable insights into mechanisms host immunity defense, many emerged from mutants defective well-characterized factors. foundational – advances high-throughput support unbiased screens fly. To investigate interplay exploit tractability host, we high-throughput, genome-wide mutant find enable pathogen, reveals mediators establishment some mouse systems. findings utility massively parallel provide platform aligning toolkit genomics.

Язык: Английский

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