CIDE proteins and their regulatory mechanisms in lipid droplet fusion and growth

FEBS Letters, Год журнала: 2024, Номер 598(10), С. 1154 - 1169

Опубликована: Фев. 14, 2024

The cell death‐inducing DFF45‐like effector (CIDE) proteins, including Cidea, Cideb, and Cidec/Fsp27, regulate various aspects of lipid homeostasis, storage, lipolysis, secretion. This review focuses on the physiological roles CIDE proteins based studies knockout mouse models human patients bearing mutations. primary cellular function is to localize droplets (LDs) control LD fusion growth across different types. We propose a four‐step process fusion, characterized by (a) recruitment surface movement, (b) enrichment condensate formation form plates at LD–LD contact sites, (c) transfer through lipid‐permeable passageways within plates, (d) completion fusion. Lastly, we outline CIDE‐interacting as regulatory factors, well their contribution in

Язык: Английский

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Lipid Droplets Big and Small: Basic Mechanisms That Make Them All

Annual Review of Cell and Developmental Biology, Год журнала: 2024, Номер 40(1), С. 143 - 168

Опубликована: Окт. 2, 2024

Lipid droplets (LDs) are dynamic storage organelles with central roles in lipid and energy metabolism. They consist of a core neutral lipids, such as triacylglycerol, which is surrounded by monolayer phospholipids specialized surface proteins. The composition determines many the LD properties, size, subcellular distribution, interaction partner organelles. Considering diverse energetic metabolic demands various cell types, it not surprising that LDs highly heterogeneous within between types. Despite their diversity, all share common biogenesis mechanism. However, adipocytes have evolved specific adaptations these basic mechanisms, enabling regulation metabolism at both cellular organismal levels. Here, we discuss recent advances understanding general mechanisms adipocyte-specific controlling fascinating

Язык: Английский

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The obesogenic effects of Bisphenol A and its analogues are differentially regulated via PPARγ transactivation in mouse 3T3-L1 cells

Toxicology in Vitro, Год журнала: 2025, Номер unknown, С. 106009 - 106009

Опубликована: Янв. 1, 2025

Язык: Английский

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Homocysteine Promotes the Pathogenesis of Atherosclerosis through the Circ‐PIAS1‐5/miR‐219a‐2‐3p/TEAD1 Axis

Advanced Science, Год журнала: 2025, Номер unknown

Опубликована: Март 16, 2025

Abstract Previous studies have established a possible link between hyperhomocysteinemia (HHcy) and dyslipidemia. Circular RNAs (circRNAs) play important regulatory roles in the development of atherosclerosis. However, biological functions potential molecular mechanisms circRNAs HHcy‐induced lipid accumulation leading to atherosclerosis are still unclear. In this study, it is determined that homocysteine (Hcy) downregulates expression circ‐PIAS1‐5 by global circRNA profiling inhibits Hcy‐mediated foam cells pathogenesis acting as sponge for miR‐219a‐2‐3p. Circ‐PIAS1‐5 identified diagnostic biomarker HHcy‐associated male “apolipoprotein E knockout (ApoE −/− )” mice. Mechanistically, activates adenosine 5‘‐monophosphate (AMP)‐activated protein kinase pathway regulating TEAD1 through miR‐219a‐2‐3p, Hcy mediates m 6 A modification nuclear export via YTHDC1 increase accelerate Taken together, these results highlight role suggest its application prognostic induced HHcy.

Язык: Английский

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Genetic and Metabolic Factors Influencing Skin Yellowness in Yellow-Feathered Broilers

Poultry Science, Год журнала: 2024, Номер 104(1), С. 104534 - 104534

Опубликована: Ноя. 8, 2024

The degree of yellowness the skin is an important factor affecting market popularity and sales price yellow-feathered broilers. Despite its commercial importance, specific pigments genetic mechanisms involved remain unclear. This study identified lutein as primary carotenoid in established serum concentration a molecular marker for predicting carcasses. Through RNA sequencing broilers with varying yellowness, we key genes like CYP26A1, CYP1B1, CYP2C18, CYP2W1, HSD17B2, AOX1, KMO, PLIN1, RET, which may regulate absorption deposition. Additionally, single nucleotide polymorphism CYP1A1 gene was significantly associated Ma-Huang chickens. Overall, this examined pigment types that influence broilers, emphasizing can serve providing insights into regulatory factors yellowness. These findings provide essential theoretical support breeding color traits

Язык: Английский

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Surface tension–driven sorting of human perilipins on lipid droplets

The Journal of Cell Biology, Год журнала: 2024, Номер 223(12)

Опубликована: Сен. 19, 2024

Perilipins (PLINs), the most abundant proteins on lipid droplets (LDs), display similar domain organization including amphipathic helices (AH). However, five human PLINs bind different LDs, suggesting modes of interaction. We established a minimal system whereby artificial LDs covered with defined polar lipids were transiently deformed to promote surface tension. Binding purified PLIN3 and PLIN4 AH was strongly facilitated by tension but poorly sensitive phospholipid composition presence diacylglycerol. Accordingly, LD coverage increased as decreased. In contrast, PLIN1 bound readily fully phospholipids; PLIN2 showed an intermediate behavior between PLIN3. adipocytes, PLIN3/4 found in soluble pool relocated upon stimulation fast triglyceride synthesis, whereas localized pre-existing consistent large difference avidity observed vitro. conclude that PLIN repertoire is adapted handling properties.

Язык: Английский

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Lipid droplets as cell fate determinants in skeletal muscle

Trends in Endocrinology and Metabolism, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 1, 2024

Язык: Английский

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The Stressogenic Impact of Bacterial Secretomes Is Modulated by the Size of the Milk Fat Globule Used as a Substrate

Foods, Год журнала: 2024, Номер 13(15), С. 2429 - 2429

Опубликована: Авг. 1, 2024

Milk fat globules (MFGs) are produced by mammary epithelial cells (MECs) and originate from intracellular lipid droplets with a wide size distribution. In the gland milk, bacteria can thrive on MFGs. Herein, we aimed to investigate whether response of MECs bacterial secretome is dependent MFG used as substrate for bacteria, differs between pathogenic commensal bacteria. We secretomes both

Язык: Английский

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In situ two-photon fluorescence spectroscopy to precisely reveal intracellular lipid droplet microenvironment via a carbonized polymer dot nanosensor

Sensors and Actuators B Chemical, Год журнала: 2024, Номер 424, С. 136900 - 136900

Опубликована: Ноя. 5, 2024

Язык: Английский

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Steric Repulsion Counteracts ER-to-Lipid Droplet Protein Movement

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Ноя. 15, 2024

Abstract Lipid droplets (LDs) are uniquely shaped organelles consisting of a neutral lipid core surrounded by phospholipid monolayer, continuous with the cytosolic leaflet endoplasmic reticulum (ER). The dynamics and function LDs closely tied to their proteome composition, which is subject dynamic remodeling. Key proteins essential for LD biology relocate from ER LDs, yet mechanisms governing movement accumulation in remain poorly understood. Here, we developed an innovative ex vivo tool quantify classify based on affinity LDs. We found broad spectrum ER-to-LD partitioning affinities. identified steric hindrance as key factor regulating protein transfer, where only slightly higher can effectively displace those lower surface. Consistent this model, observed that differentiation 3T3 pre-adipocytes into adipocytes involves extensive remodeling targeting Plin1—a high-affinity protein—becoming predominantly recruited excluding other proteins. These findings highlight lateral protein-protein exclusion fundamental mechanism shaping proteome, providing new insights biogenesis function.

Язык: Английский

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