IDR-driven TOLLIP condensates antagonize the innate antiviral immunity by promoting the deSUMOylation of MAVS

Cell Reports, Год журнала: 2025, Номер 44(3), С. 115348 - 115348

Опубликована: Фев. 27, 2025

Язык: Английский

---

Regulation of viral replication by host restriction factors

Frontiers in Immunology, Год журнала: 2025, Номер 16

Опубликована: Янв. 23, 2025

Viral infectious diseases, caused by numerous viruses including severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), influenza A virus (IAV), enterovirus (EV), human immunodeficiency (HIV), hepatitis B (HBV), and papillomavirus (HPV), pose a continuous threat to global health. As obligate parasites, rely on host cells replicate, have developed defense mechanisms counteract viral infection. Host restriction factors (HRFs) are critical components of the early antiviral response. These cellular proteins inhibit replication spread impeding essential steps in life cycle, such as entry, genome transcription replication, protein translation, particle assembly, release. This review summarizes current understanding how with primary focus their diverse against range viruses, SARS-CoV-2, virus, enteroviruses, papillomavirus. In addition, we highlight crucial role these shaping host-virus interactions discuss potential targets for drug development.

Язык: Английский

---

Ubiquitin-proteasome system: a potential participant and therapeutic target in antiphospholipid syndrome

Frontiers in Immunology, Год журнала: 2025, Номер 16

Опубликована: Фев. 18, 2025

APS (antiphospholipid syndrome) is an autoimmune disease characterized by thrombosis, pregnancy complications and persistent elevation of aPLs antibodies). Dysfunction innate immune cells, ECs (endothelial cells), platelets trophoblast cells are central to the development APS. The UPS (ubiquitin-proteasome system) a highly conserved post-translational modification in eukaryotes. Imbalance potentially disrupts protein homeostasis network provokes prothrombotic proinflammatory signaling during progression. In vivo, low-dose proteasome inhibitors believed effectively inhibit production factors clinical manifestations this review, we would like summarize likely contribution dysregulated pathogenesis Given significant progress made understanding molecular mechanisms how alterations lead diseases, targeting may represent novel therapeutic strategy.

Язык: Английский

---

Liquid-liquid phase separation of DDX3X: mechanisms, pathological implications, and therapeutic potential

International Journal of Biological Macromolecules, Год журнала: 2025, Номер 317, С. 144835 - 144835

Опубликована: Май 31, 2025

Язык: Английский

---

Decellularized adipose-derived matrix from Superficial layers of abdominal adipose tissue exhibits superior capacity of adipogenesis compared to deep layers

Materials Today Bio, Год журнала: 2024, Номер 28, С. 101235 - 101235

Опубликована: Сен. 12, 2024

Язык: Английский

---

RPS 2.0: an updated database of RNAs involved in liquid–liquid phase separation

Nucleic Acids Research, Год журнала: 2024, Номер 53(D1), С. D299 - D309

Опубликована: Окт. 26, 2024

Liquid-liquid phase separation (LLPS) is a crucial process for the formation of biomolecular condensates such as coacervate droplets, P-bodies and stress granules, which play critical roles in many physiological pathological processes. Increasing studies have shown that not only proteins but also RNAs role LLPS. To host LLPS-associated RNAs, we previously developed database named 'RPS' 2021. In this study, present an updated version RPS 2.0 (https://rps.renlab.cn/) to incorporate newly generated data new driven by post-transcriptional regulatory mechanisms. Currently, hosts 171 301 entries 24 different with four evidence types, including 'Reviewed', 'High-throughput (LLPS enrichment)', perturbation)' 'Predicted', five event 'Expression', 'APA', 'AS', 'A-to-I' 'Modification'. Additionally, extensive annotations are provided 2.0, RNA sequence structure features, RNA-protein/RNA-RNA interactions, modifications, well diseases related annotations. We expect will further promote research deepen our understanding biological functions mechanisms

Язык: Английский

---

Induction of translation-suppressive G3BP1+ stress granules and interferon-signaling cGAS condensates by transfected plasmid DNA

hLife, Год журнала: 2024, Номер 3(1), С. 21 - 37

Опубликована: Дек. 24, 2024

Plasmid DNA transfection is one of the fundamental tools biomedical research. Here, we found that plasmid mediated by liposomes activates multiple innate immune responses in several widely used cell lines. Their activations were visible detection stress granules (SG) and cGAS-DNA condensates (cGC) transfected cells a dose-dependent manner. The elevated levels phosphorylated eukaryotic translation initiation factor 2 subunit alpha (eIF2α), interferon regulatory 3 (IRF3), signal transducer activator transcription 1 (STAT1) induced DNA-transfected cells. formation SG but not cGC required active dsRNA DNA-induced or mutually exclusive because triggering two distinct pathways. Knockdown (KD) PKR before led to abolish without affecting formation. Conversely, cGAS KD could prevent In addition, be prevented, respectively, co-expression KSHV proteins ORF57 (PKR inhibitor) ORF52 (cGAS inhibitor). Inhibition KD, also increased expression transgenes, indicating activation represents major roadblock gene expression. Together, these data indicate triggers causes significant cellular perturbation should considered during experiment design interpretation.

Язык: Английский

---

USP8 suppresses porcine reproductive and respiratory syndrome virus replication by positively regulating MAVS mediated Ⅰ-IFN signaling

Veterinary Microbiology, Год журнала: 2024, Номер 298, С. 110286 - 110286

Опубликована: Окт. 30, 2024

Язык: Английский

---