A feedback-driven brain organoid platform enables automated maintenance and high-resolution neural activity monitoring
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Март 17, 2024
The
analysis
of
tissue
cultures,
particularly
brain
organoids,
requires
a
sophisticated
integration
and
coordination
multiple
technologies
for
monitoring
measuring.
We
have
developed
an
automated
research
platform
enabling
independent
devices
to
achieve
collaborative
objectives
feedback-driven
cell
culture
studies.
Our
approach
enables
continuous,
communicative,
non-invasive
interactions
within
Internet
Things
(IoT)
architecture
among
various
sensing
actuation
devices,
achieving
precisely
timed
control
in
vitro
biological
experiments.
framework
integrates
microfluidics,
electrophysiology,
imaging
maintain
cerebral
cortex
organoids
while
measuring
their
neuronal
activity.
are
cultured
custom,
3D-printed
chambers
affixed
commercial
microelectrode
arrays.
Periodic
feeding
is
achieved
using
programmable
microfluidic
pumps.
computer
vision
fluid
volume
estimator
used
as
feedback
rectify
deviations
perfusion
during
media
feeding/aspiration
cycles.
validated
the
system
with
set
7-day
studies
mouse
comparing
manual
protocols.
protocols
were
maintaining
robust
neural
activity
throughout
experiment.
enabled
hourly
electrophysiology
recordings
Median
unit
firing
rates
increased
every
sample
dynamic
patterns
organoid
revealed
by
high-frequency
recordings.
Surprisingly,
did
not
affect
rate.
Furthermore,
performing
exchange
recording
showed
no
acute
effects
on
rate,
use
this
reagent
screening
Язык: Английский
The flow of reward information through neuronal ensembles in the accumbens
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Фев. 16, 2024
Summary
Reward
information
flows
through
neuronal
ensembles
in
the
nucleus
accumbens
shell
(NAcSh),
influencing
decision-making.
We
investigated
this
phenomenon
by
training
rats
a
self-guided
probabilistic
choice
task
while
recording
single-unit
activity
NAcSh.
found
that
dynamically
adapted
their
choices
based
on
an
internal
representation
of
reward
likelihood.
Neuronal
NAcSh
act
as
dynamic
modules
to
process
different
aspects
reward-guided
behavior.
Ensembles
change
composition
and
functional
connections
throughout
reinforcement
learning.
The
forms
highly
connected
network
with
heavy-tailed
distribution
hubs,
facilitating
efficient
flow.
delivery
evokes
higher
mutual
between
unifies
activity,
omission
leads
less
synchronization.
Our
recordings
shed
light
how
propagates
changing
neurons
These
exhibit
flexible
membership,
dropping
out
even
shrinking
number
rat
learns
obtain
(energy)
rewards
ever-changing
environment.
Graphical
Abstract
Язык: Английский
The flow of reward information through neuronal ensembles in the accumbens
Cell Reports,
Год журнала:
2024,
Номер
43(10), С. 114838 - 114838
Опубликована: Окт. 1, 2024
Highlights•NAcSh
neuronal
ensembles
act
as
distinct
modules
to
process
reward-guided
behavior•NAcSh
network
exhibits
hubs
and
small-world
heavy-tailed
distribution
properties•Sucrose
evokes
higher
mutual
information
(MI)
in
the
NAcSh
than
omission•Learning
strengthens
MI
during
reward
delivery
weakens
it
omissionSummaryThe
nucleus
accumbens
shell
(NAcSh)
integrates
through
diverse
specialized
ensembles,
influencing
decision-making.
By
training
rats
a
probabilistic
choice
task
recording
activity,
we
found
that
adapt
their
choices
based
solely
on
presence
or
absence
of
sucrose
reward,
suggesting
they
build
an
internal
representation
likelihood.
We
further
demonstrate
dynamically
different
aspects
behavior,
with
changes
composition
functional
connections
observed
throughout
reinforcement
learning
process.
The
forms
highly
connected
characterized
by
hubs,
facilitating
efficient
flow.
Reward
enhances
information,
indicating
increased
communication
between
synchronization,
whereas
omission
decreases
it.
Our
findings
reveal
how
flows
dynamic
whose
flexible
membership
adapts
rat
learns
obtain
rewards
(energy)
ever-changing
environment.Graphical
abstract
Язык: Английский
Protosequences in human cortical organoids model intrinsic states in the developing cortex
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2023,
Номер
unknown
Опубликована: Дек. 30, 2023
Neuronal
firing
sequences
are
thought
to
be
the
basic
building
blocks
of
neural
coding
and
information
broadcasting
within
brain.
However,
when
emerge
during
neurodevelopment
remains
unknown.
We
demonstrate
that
structured
present
in
spontaneous
activity
human
murine
brain
organoids
ex
vivo
neonatal
slices
from
somatosensory
cortex.
observed
a
balance
between
temporally
rigid
flexible
patterns
emergent
phenomena
early
postnatal
cortex,
but
not
primary
dissociated
cortical
cultures.
Our
findings
suggest
temporal
do
arise
an
experience-dependent
manner,
rather
constrained
by
innate
preconfigured
architecture
established
neurogenesis.
These
highlight
potential
for
further
explore
how
exogenous
inputs
can
used
refine
neuronal
circuits
enable
new
studies
into
genetic
mechanisms
govern
assembly
functional
circuitry
development.
Язык: Английский