Loss of MEGF10 Decreases the Number of Perisynaptic Schwann Cells and Innervation of Neuromuscular Junctions in Aging Mice
Journal of the Peripheral Nervous System,
Год журнала:
2025,
Номер
30(1)
Опубликована: Март 1, 2025
ABSTRACT
Background
and
Aims
At
the
neuromuscular
junction
(NMJ),
synapse
between
motor
neurons
muscle
fibers,
reside
perisynaptic
Schwann
cells
(PSCs)
which
are
specialized
glia
that
regulate
maintenance
repair
of
this
synapse.
While
we
know
how
PSC
morphology
numbers
change
in
aging
various
disorders
adversely
affect
NMJ,
molecular
mechanisms
alter
functions
remain
unknown.
In
study,
investigated
whether
MEGF10
PSCs
modulates
NMJ
stability
developing,
healthy
young
adult,
middle‐aged,
axotomized
mice.
is
a
glial
phagocytic
receptor
enriched
compared
to
other
(SCs).
Methods
We
isolated
from
transgenic
reporter
mouse
line
assess
Megf10
expression
at
different
ages
following
nerve
injury
using
qPCR.
then
used
conditional
lacking
all
SCs,
including
(
SC‐KO
mice).
examined
NMJs
axonal
debris
clearance
mice
confocal
microscopy.
Results
found
peaks
during
development
decreases
denervation
NMJs.
were
morphologically
normal
developing
adult
This
was
not
case
middle‐aged
mice,
presented
with
fewer
PSCs,
decreased
coverage
endplate,
innervation
comparison
control
Following
injury‐induced
damage,
cleared
faster
mice;
yet,
rate
reinnervation
unchanged
Interpretation
The
data
study
suggest
maintain
number
aging.
also
suggests
important
roles
for
mediating
injury.
Язык: Английский
Dscamb regulates cone mosaic formation in zebrafish via filopodium-mediated homotypic recognition
Nature Communications,
Год журнала:
2025,
Номер
16(1)
Опубликована: Март 25, 2025
Cone
photoreceptors
assemble
to
form
a
regular
mosaic
pattern
in
vertebrate
retinas.
In
zebrafish,
four
distinct
spectral
cone
types
(red,
green,
blue,
and
ultraviolet),
lattice-like
pattern.
However,
the
mechanism
of
formation
has
been
unknown.
Here
we
show
that
Down
Syndrome
Cell
Adhesion
Molecule
b
(Dscamb)
regulates
especially
via
red-cone
spacing.
During
photoreceptor
differentiation,
newly
formed
cones
extend
filopodium-like
processes
laterally
apical
surfaces
neighboring
cones.
Interestingly,
red
filopodia,
but
promptly
retract
them
when
they
meet
their
own
type,
suggesting
filopodium-mediated,
homotypic
recognition
self-avoidance.
This
self-avoidance
is
compromised
zebrafish
dscamb
mutants,
leading
abnormal
clustering
subsequent
disruption
Thus,
filopodium-mediated
spacing
same
type
depends
on
Dscamb
essential
for
zebrafish.
retina.
The
authors
report
(DSCAM)
mediates
between
Язык: Английский
Retinal ganglion cell-derived semaphorin 6A segregates starburst amacrine cell dendritic scaffolds to organize the inner retina
Development,
Год журнала:
2024,
Номер
151(22)
Опубликована: Ноя. 4, 2024
ABSTRACT
To
form
functional
circuits,
neurons
must
settle
in
their
appropriate
cellular
locations,
and
then
project
elaborate
neurites
to
contact
target
synaptic
neuropils.
Laminar
organization
within
the
vertebrate
retinal
inner
plexiform
layer
(IPL)
facilitates
pre-
postsynaptic
neurite
targeting,
yet
precise
mechanisms
underlying
establishment
of
IPL
subdomains
are
not
well
understood.
Here,
we
explore
defining
compartmentalization
OFF
ON
generally,
direction-selective
specifically,
developing
mouse
IPL.
We
show
that
semaphorin
6A
(Sema6A),
a
repulsive
axon
guidance
cue,
is
required
for
delineation
versus
circuits
IPL:
Sema6a
null
IPL,
boundary
between
domains
blurred.
Furthermore,
Sema6A
expressed
by
ganglion
cells
(RGCs)
directs
laminar
segregation
starburst
amacrine
cell
dendritic
scaffolds,
which
themselves
serve
as
substrate
upon
other
elaborate.
These
results
demonstrate
RGCs,
first
type
neuron
born
retina,
play
an
active
role
specialization
Язык: Английский