Zero-echo time imaging achieves whole brain activity mapping without ventral signal loss in mice

NeuroImage, Год журнала: 2025, Номер unknown, С. 121024 - 121024

Опубликована: Янв. 1, 2025

Functional MRI (fMRI) is an important tool for investigating functional networks. However, the widely used fMRI with T2*-weighted imaging in rodents has problem of signal lack lateral ventral area forebrain including amygdala, which essential not only emotion but also noxious pain. Here, we scouted zero-echo time (ZTE) sequence, robust to magnetic susceptibility and motion-derived artifacts, image activation whole brain amygdala following stimulation hind paw. ZTE exhibited higher spatial temporal signal-to-noise ratios than conventional sequences. Electrical sensory paw evoked increase primary somatosensory cortex. Formalin injection into early latent change signals throughout subregions amygdala. Furthermore, resting-state using demonstrated connectivity, that These results indicate feasibility first show acute activity different subnuclei complex after nociceptive stimulation.

Язык: Английский

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Structural and functional connectivity coupling as an imaging marker for bone metastasis pain in lung cance patients

Brain Research Bulletin, Год журнала: 2025, Номер unknown, С. 111210 - 111210

Опубликована: Янв. 1, 2025

Язык: Английский

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Neurotransmitter Imbalance and Amygdala Synaptic Plasticity in Lumbar Disc Herniation-induced Chronic Pain and Related Emotional Disturbances:A multi-omics analysis

Neuropharmacology, Год журнала: 2025, Номер unknown, С. 110405 - 110405

Опубликована: Март 1, 2025

Chronic pain due to lumbar disc herniation (LDH) significantly impairs quality of life and is often accompanied by emotional disturbances, such as anxiety depression. Despite the recognition these comorbidities, underlying neural mechanisms remain unclear. This study investigates role neurotransmitter imbalances key regulatory molecules in LDH-induced chronic related with a focus on synaptic plasticity amygdala. A rat model LDH was developed using male Sprague-Dawley rats. Behavioral assessments were conducted evaluate hypersensitivity, anxiety, depression-like behaviors. Cerebrospinal fluid (CSF) metabolomics amygdala transcriptomics employed analyze profiles gene expression. In vitro experiments explore PRKCG plasticity. tests showed significant hypersensitivity anxiety- behaviour Metabolomic analysis revealed altered levels glutamate γ-aminobutyric acid (GABA) CSF, indicating imbalances. Transcriptomic profiling identified changes genes plasticity, including PRKCG. knockdown led reduced CAMKII phosphorylation GRIA1 expression, supporting its modulating provides evidence that alterations within may contribute persistence associated disturbances LDH. represent novel therapeutic target for treating both disturbances.

Язык: Английский

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Apremilast reduces co-occurring alcohol drinking and mechanical allodynia and regulates central amygdala GABAergic transmission

JCI Insight, Год журнала: 2025, Номер 10(8)

Опубликована: Апрель 21, 2025

The FDA-approved phosphodiesterase type 4 (PDE4) inhibitor, apremilast, has been recently investigated as a pharmacotherapy for alcohol use disorder (AUD) with promising efficacy in rodent models and humans. However, apremilast's effects on mechanical allodynia associated AUD well distinct responses of this drug between males females are understudied. present study examined the behavioral electrophysiological apremilast Marchigian Sardinian alcohol-preferring (msP) rats their Wistar counterparts. We used 2-bottle choice (2-BC) drinking procedure tested sensitivity across our regimen. Spontaneous inhibitory GABA-mediated postsynaptic currents from central nucleus amygdala (CeA) following application were subset using ex vivo electrophysiology. Transcript levels Pde4a or -4b subtypes assessed modulation by alcohol. Apremilast reduced both strains rats. immediately after drinking, persisting into early late abstinence. increased GABAergic transmission CeA slices alcohol-exposed Wistars but not msP rats, suggesting neuroadaptations msPs excessive allodynia. Pde4 subtype transcript These results suggest that alleviates co-occurring pain sensitivity, they further confirm PDE4's role pain-associated AUD.

Язык: Английский

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The neuroplastic brain: current breakthroughs and emerging frontiers

Brain Research, Год журнала: 2025, Номер unknown, С. 149643 - 149643

Опубликована: Апрель 1, 2025

Язык: Английский

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Crosstalk Among Gut Microbiota, Fecal Metabolites, and Amygdala Neuropathology Genes After Ginger Polyphenol Administration in Female Rats with Neuropathic Pain: Evidence for Microbiota–Gut–Brain Connection

Nutrients, Год журнала: 2025, Номер 17(9), С. 1444 - 1444

Опубликована: Апрель 25, 2025

Objectives. The relationships among neuropathic pain, gut microbiota, microbiome-derived metabolites, and neuropathology have received increasing attention. This study examined the effects of two dosages gingerol-enriched ginger (GEG) on mechanical hypersensitivity, anxiety-like behavior, microbiome composition its markers in female rats spinal nerve ligation (SNL) model pain. Methods. Forty were assigned to 4 groups: sham-vehicle, SNL-vehicle, SNL+GEG at 200 mg/kg BW, 600 BW via oral gavage. All animals given an AIN-93G diet for 5 weeks. Mechanical hypersensitivity was assessed by von Frey test. Anxiety-like behavior open field Fecal microbiota metabolites determined using 16S rRNA gene sequencing GC-MS, respectively. Neuropathology expression profiling amygdala nCounter® pathway panel. Results. Both GEG-treated groups showed decreased SNL model. Gut diversity both GEG compared with untreated rats. In model, phyla such as Bacteroidota, Proteobacteria Verrucomicrobiota decreased. Compared group, exhibited increased abundance Bacteroidota (i.e., Rikenella, Alistipes, Muribaculaceae, Odoribacter), Firmicutes UBA1819, Ruminococcaceae, Oscillospiraceae, Roseburia), Victivallis). had higher levels nine hydrophilic positive [val-glu, urocanic acid, oxazolidinone, L-threonine, L-norleucine, indole, imino-tryptophan, 2,3-octadiene-5,7-diyn-1-ol, (2E)-3-(3-hydroxyphenyl) acrylaldehyde] negative [methylmalonic acid metaphosphoric acid], well lower five [xanthine, N-acetylmuramic doxaprost, adenine, 1-myristoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine]. Among 770 genes, 1 (PLA2G4A) upregulated 2 genes (CDK5R1 SHH) downregulated caused upregulation (APC, CCNH, EFNA5, GRN, HEXB, ITPR1, PCSK2, TAF9, WFS1) downregulation three (AVP, C4A, TSPO) amygdala. Conclusions. supplementation mitigated pain-associated behaviors part reversing molecular signature associated changes fecal which could play a role mediating

Язык: Английский

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Corticotropin-releasing hormone neurons in the central Amygdala: An integrative hub for pain, emotion, and addiction neurobiology

Brain Research, Год журнала: 2025, Номер unknown, С. 149753 - 149753

Опубликована: Июнь 1, 2025

Язык: Английский

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