Unraveling Asthma Through Single-Cell RNA Sequencing in Understanding Disease Mechanisms
Journal of Asthma,
Год журнала:
2025,
Номер
unknown, С. 1 - 15
Опубликована: Фев. 27, 2025
To
elucidate
the
fundamental
principles
of
single-cell
RNA
sequencing
(scRNA-seq)
and
summarize
its
application
in
asthma
research,
aiming
to
enhance
understanding
pathophysiology
guide
future
research
directions.Datasource:Recent
advances
emerging
scRNA-seq
role
pathogenesis
asthma.Study
Selections:This
review
incorporates
studies
that
analyzed
heterogeneity
cell
types
their
functional
states
using
scRNA-seq,
with
particular
emphasis
on
immune
cells
airway
remodeling.
The
selection
specific
markers
was
based
relevance
pathogenesis,
we
discuss
rationale
for
favoring
certain
technologies
these
investigations.
ScRNA-seq
technology
has
provided
insights
into
key
mechanisms
underlying
inflammation
remodeling
asthma.
It
uncovered
diversity
subtypes
roles
revealing
critical
pathways
contribute
disease
progression.
These
findings
offer
a
theoretical
foundation
development
targeted
therapeutic
strategies,
paving
way
personalized
medicine
improved
patient
outcomes.
reveals
complex
asthma,
offering
potential
therapies.
However,
challenges
remain,
such
as
need
further
refinement
data
integration
methods
addressing
limited
clinical
applicability
current
findings.
Future
should
focus
overcoming
limitations,
improving
type
annotation,
expanding
include
longitudinal
better
understand
dynamics
therapy
responses.
Язык: Английский
Epithelial FETUB-mediated the inhibition of NEP activity aggravates asthma
International Immunopharmacology,
Год журнала:
2025,
Номер
152, С. 114397 - 114397
Опубликована: Март 9, 2025
Язык: Английский
Comprehensive sequencing of the lung neuroimmune landscape in response to asthmatic induction
Frontiers in Immunology,
Год журнала:
2025,
Номер
16
Опубликована: Март 13, 2025
Introduction
Evidence
demonstrates
that
sensory
neurons
respond
to
pathogenic/allergic
infiltration
and
mediate
immune
responses,
forming
an
integral
part
of
host
defense
becomes
hypersensitized
during
allergy.
Our
objective
was
investigate
how
asthmatic
induction
alters
the
pulmonary
neuroimmune
transcriptome.
We
hypothesized
would
upregulate
genes
in
vagal
ganglia
(nodose/jugular
ganglia),
which
be
associated
with
immunity,
these
clustered,
primarily
nodose
neurons.
Furthermore,
lungs
increase
transcripts
nerve
activation,
centered
neural
neuroendocrine-like
cells.
Methods
Standard
RNA
sequencing,
single
nucleus-RNA
spatial
sequencing
ganglia.
RNA-sequencing
naïve
mice
have
undergone
Alternaria
alternata
.
Results
Bulk
RNA-seq
revealed
related
allergen
sensing
were
increased
nodose/jugular
compared
control
These
clusters
as
shown
by
single-nucleus
a
distinct
caudal-to-rostral
arrangement
presented
delineated
transcriptomics.
The
closely
match
previous
identification
neuron
clusters.
Correspondingly,
lung
transcriptome
altered
such
excitation
upregulated.
distribution
transcriptomics
illustrate
expressed
cells/club
cells,
Conclusions
results
show
is
response
cell
cluster
spatially
manner.
Язык: Английский
Role of specialized sensory neuron subtypes in modulating peripheral immune responses
Immunity,
Год журнала:
2025,
Номер
unknown
Опубликована: Май 1, 2025
Язык: Английский
TUMOR-INFILTRATING NOCICEPTOR NEURONS PROMOTE IMMUNOSUPPRESSION
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Авг. 26, 2024
Nociceptor
neurons
impact
tumor
immunity.
Removing
nociceptor
reduced
myeloid-derived
suppressor
cell
(MDSCs)
infiltration
in
mouse
models
of
head
and
neck
carcinoma
melanoma.
Carcinoma-released
small
extracellular
vesicles
(sEVs)
attract
nociceptive
nerves
to
tumors.
sEV-deficient
tumors
fail
develop
mice
lacking
neurons.
Exposure
dorsal
root
ganglia
(DRG)
cancer
sEVs
elevated
expression
Substance
P,
IL-6
injury-related
neuronal
markers
while
treatment
with
cytotoxic
CD8
T-cells
induced
an
immunosuppressive
state
(increased
exhaustion
ligands
cytokines).
Cancer
patient
enhanced
DRG
responses
capsaicin,
indicating
increased
sensitivity.
Conditioned
media
from
co-cultures
promoted
MDSC
primary
bone
marrow
cells
conditioned
together
checkpoint
on
T-cells.
Our
findings
indicate
that
facilitate
CD8+
T
enhance
infiltration.
Targeting
nociceptor-released
emerges
as
a
novel
strategy
disrupt
harmful
neuro-immune
interactions
anti-tumor
Язык: Английский