MAGIC matrices: freeform bioprinting materials to support complex and reproducible organoid morphogenesis
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Фев. 5, 2024
Organoids
are
powerful
models
of
tissue
physiology,
yet
their
applications
remain
limited
due
to
relatively
simple
morphology
and
high
organoid-to-organoid
structural
variability.
To
address
these
limitations
we
developed
a
soft,
composite
yield-stress
extracellular
matrix
that
supports
optimal
organoid
morphogenesis
following
freeform
3D
bioprinting
cell
slurries
at
tissue-like
densities.
The
material
is
designed
with
two
temperature
regimes:
4
°C
it
exhibits
reversible
behavior
support
long
printing
times
without
compromising
viability.
When
transferred
culture
37
°C,
the
cross-links
similar
viscoelasticity
plasticity
basement
membrane
extracts
such
as
Matrigel.
We
first
characterize
rheological
properties
MAGIC
matrices
optimize
morphogenesis,
including
low
stiffness
stress
relaxation.
Next,
combine
this
custom
piezoelectric
printhead
allows
more
reproducible
robust
self-organization
from
uniform
spatially
organized
"seeds."
apply
for
high-throughput
generation
intestinal,
mammary,
vascular,
salivary
gland,
brain
arrays
structurally
those
grown
in
pure
Matrigel,
but
exhibit
dramatically
improved
homogeneity
size,
shape,
maturation
time,
efficiency
morphogenesis.
flexibility
method
enabled
fabrication
fully
microphysiological
systems,
perfusable
tubes
experience
cyclic
strain
response
pressurization.
Furthermore,
reproducibility
structure
increased
statistical
power
drug
assay
by
up
8
orders-of-magnitude
given
number
comparisons.
Combined,
advances
lay
foundation
efficient
complex
morphologies
canalizing
both
space
time.
Язык: Английский
Rho/ROCK activity tunes cell compartment segregation and differentiation in nephron-forming niches
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2023,
Номер
unknown
Опубликована: Ноя. 10, 2023
Controlling
the
time
and
place
of
nephron
formation
Язык: Английский
Controlling Cell Interactions with DNA Directed Assembly
Advanced Healthcare Materials,
Год журнала:
2024,
Номер
unknown
Опубликована: Окт. 14, 2024
Abstract
The
creation
of
complex
cellular
environments
is
critical
to
mimicking
tissue
that
will
play
a
role
in
next‐generation
engineering,
stem
cell
programming,
and
therapeutic
screening.
To
address
this
growing
need,
techniques
capable
manipulating
cell–cell
cell‐material
interactions
are
required
span
single‐cell
3D
architectures.
DNA
programmed
assembly
placement
cells
present
powerful
technique
for
the
bottom‐up
synthesis
living
microtissues
probing
key
questions
cell‐material‐driven
behaviors
through
its
refined
control
over
architecture.
This
review
examines
current
state
art
programming
with
applications
spanning
model
building,
fundamental
biology,
manipulation
measurements
across
host
applications.
Язык: Английский
Engineering kidney developmental trajectory using culture boundary conditions
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Дек. 16, 2024
Abstract
Kidney
explant
cultures
are
traditionally
carried
out
at
air-liquid
interfaces,
which
disrupts
3D
tissue
structure
and
limits
interpretation
of
developmental
data.
To
overcome
this
limitation,
we
developed
a
culture
technique
using
hydrogel
embedding
to
capture
morphogenesis
in
real
time.
We
show
that
better
approximates
vivo
-like
niche
spacing
dynamic
tubule
tip
rearrangement,
as
well
presentation
branching
defects
under
perturbations
glial
cell-derived
neurotrophic
factor
(GDNF)-
RE
arranged
during
T
ransfection
(RET)
tyrosine
kinase
signaling.
find
the
concentration
matrix
influences
number
nephrons
per
ureteric
bud
(UB)
between
tips.
isolate
effect
specific
material
properties
on
development,
introduce
engineered
acrylated
hyaluronic
acid
hydrogels
allow
independent
tuning
stiffness
adhesion.
sufficient
adhesion
both
required
maintain
kidney
shape.
Matrix
has
“Goldilocks
effect”
nephron
UB
balance
centered
∼2
kPa,
while
higher
increases
ratio.
Our
captures
large-scale,
3D,
providing
platform
suited
contrasting
normal
congenital
disease
contexts.
Moreover,
understanding
impact
boundary
condition
mechanics
development
benefits
fundamental
renal
research
advances
engineering
next-generation
replacement
tissues.
Язык: Английский